Summary Background Cutaneous hyperpigmentation occurs in multiple conditions. In addition, many Asian women desire a lighter skin colour. Thus, there is need for the development of lightening agents. Niacinamide possible candidate. Objectives To investigate effects niacinamide on melanogenesis vitro and facial colour vivo Japanese women. Methods Melanin production was measured purified mushroom tyrosinase assay, cultured melanocytes, keratinocyte/melanocyte coculture model, pigmented...

Abstract: Skin pigmentation results in part from the transfer of melanized melanosomes synthesized by melanocytes to neighboring keratinocytes. Plasma membrane lectins and their glycoconjugates expressed these epidermal cells are critical molecules involved this process. In addition, derivative vitamin B 3 , niacinamide, can inhibit melanosome induce skin lightening. We investigated effects on viability keratinocytes reversibility melanosome‐transfer inhibition induced agents using an vitro...

Abstract Facial skin redness can be an indicator of inflammation, however the physiological connection between facial and inflammatory status, as well its role in age‐related changes, remains poorly understood. This study aims to investigate association pattern biological changes occurring skin. Four studies were conducted recruiting healthy Northern Asian females. Disordered spatial patterns signals assessed using image analysis, i.e., a * gradient algorithm, which quantifies disordered...

Background/purpose: Cutaneous hyperpigmentation occurs in multiple conditions. There is a strong need for the improvement of especially among Asian women. However, effect existing skin‐lightening agents not sufficient. One reason attributes to limited capability active be delivered transepidermally. Ultrasound one promising approach enhance transepidermal transport. In this work, we investigate use high‐frequency ultrasound together with coupling gel containing (ascorbyl glucoside and...

Summary Objectives N ‐undecyl‐10‐enoyl‐ l ‐phenylalanine (Sepiwhite ® , ‐undecylenoyl phenylalanine), a reported alpha‐melanocyte‐stimulating hormone (MSH) receptor antagonist, has been observed to reduce melanin production in cultured melanocytes. In other testing, niacinamide found inhibit melanosome transfer cells and the appearance of hyperpigmented spots clinical studies. Since these two agents function by different mechanisms, we conducted studies determine if their combination is more...

Abstract Background The accumulation of advanced glycation end products (AGEs) can impact cellular homoeostasis and protein structure, thus is implicated in numerous skin conditions including yellow, dull appearance. AGE formation irreversible; thus, understanding the recycling process AGEs critical for addressing appearance conditions. Objective To determine whether (i) occurs group among young population (age 20–29) (ii) vitro autophagy stimulation results reduction keratinocytes. Methods...

Sallow and/or dull skin appearance is greatly attributable to the yellow components of tone. Bilirubin a chromophore known be made in liver spleen and transported throughout body via blood stream. Recent publications suggest bilirubin may synthesized other cells/organs, including skin. We found human keratinocytes express transcripts involved biosynthesis. In parallel, we also could indeed synthesize monolayer 3D skin-equivalent model. The amount was substantial enough contribute yellowness....

Hyperpigmented spots are common issues in all ethnicities with a hallmark characteristic of increased melanocyte dendricity.To determine (1) potential receptors and/or cytokines that involved dendricity multiple facial spot types; (2) treatment effects skin-lightening compounds on identified cytokine release from keratinocytes and melanocytes.Facial (melasma, solar lentigo, acne-induced post-inflammatory hyperpigmentation) adjacent non-spot skin biopsies were collected Chinese women (age...

2‐Hexyldecanol has long been used in skin‐care products, but not previously reported as an active ingredient for skin benefits. To evaluate 2‐hexyldecanol vitro and ex vivo systems and, if found to be active, progress it topical clinical testing determine effects on pigmentation skin. was tested melanocyte cell culture (B16 mouse melanoma cells normal human melanocytes) its effect proteolytic activity melanin production, the absence presence of proteasome‐specific inhibitor, MG132. It...

Skin yellowness is a hallmark of dull or unhealthy skin, particularly among Asians. Previous research has indicated link between skin glycation and yellowness. However, the specific glycated chemicals contributing to yellowish appearance have not been identified yet. Using HPLC-PDA-HRMS coupled with native artificially human epidermal explant we intensely yellow colored chromophores “(1R, 8aR) (1S, 8aR)-4-(2-furyl)-7-[(2-furyl)-methylidene]-2-hydroxy-2H,7H,8AH-pyrano-[2,3-B]-pyran-3-one”...

Saccharomycopsis ferment filtrate (SFF), mainly used in skin care products, has been reported to inhibit inflammatory nitric oxide production and prevent epidermal damage. However, the effects of SFF on keratinocytes have not yet explored. We investigated barrier function using human primary keratinocytes. Cell viability was determined by MTT assay. The mRNA protein expression levels tight junction proteins (claudin-1, -3, -4, occludin, ZO-1) were analyzed RT-PCR Western blotting,...

Abstract Degradation of melanosomes in light skin ( LS , i.e. phototype I / II ) appears to occur more rapidly than dark DS IV V ). Hydrolytic enzymes known reside and be expressed a differential pattern within the interfollicular epidermis are implicated playing role epidermal differentiation potentially melanosome degradation. The aim this present study was evaluate expression hydrolytic that may correlate with physiological phenotypic differences seen between . Expression six confirmed by...

Galactomyces ferment filtrate (GFF, Pitera™) is a cosmetic ingredient known to have multiple skin care benefits, such as reducing redness and pore size via the topical application of its moisturizer form. Although GFF act partly an antioxidative agonist for aryl hydrocarbon receptor (AHR), significance in keratinocyte biology not fully understood. In this study, we conducted transcriptomic analysis GFF-treated human keratinocytes. Three different lots consistently modulated 99 (22...

Collagen type XVIII regulates cellular activities of adjacent cells at the dermal-epidermal junction (DEJ). To investigate its possible changes during aging, we compared mRNA levels and protein localization in skin samples from female participants aged 20-70 years old. In addition, evaluated beneficial effects unripe peach extracts a 3D model.Sun-exposed or sun-protected were by DNA array immunohistochemistry for basement membrane components. evaluate protective fresh extract, UV-B...

Hyperpigmented spots are common issues in all ethnicities, involving multiple intrinsic and extrinsic factors such as UVB exposure, hormone balance, inflammatory status ageing.To determine (i) melanocyte dendricity facial spot types, (ii) impact of High Mobility Group Box 1 (HMGB1), the combination sucrose dilaurate laurate (SDL) on melanogenesis dendricity, (iii) SDL effect a human use test.Facial adjacent non-spot skin biopsies were collected from Chinese women (age 20-70). Histological...