A5058548311- Signaling Pathways in Disease
- Ion channel regulation and function
- Cancer Cells and Metastasis
- Trace Elements in Health
- Effects of Radiation Exposure
- Cancer, Hypoxia, and Metabolism
- Heat shock proteins research
- Cardiac electrophysiology and arrhythmias
- Ion Transport and Channel Regulation
- Cancer-related gene regulation
- Neuroblastoma Research and Treatments
- Cancer Research and Treatments
- Lung Cancer Research Studies
- Erythrocyte Function and Pathophysiology
- Lung Cancer Treatments and Mutations
- Radiation Therapy and Dosimetry
- thermodynamics and calorimetric analyses
- Immunotherapy and Immune Responses
- Calcium signaling and nucleotide metabolism
- HER2/EGFR in Cancer Research
- Cellular transport and secretion
University of Pittsburgh
2015-2023
University of Pittsburgh Medical Center
2017-2023
UPMC Hillman Cancer Center
2012-2016
Abstract Ionizing radiation (IR) is used for patients diagnosed with unresectable non small cell lung cancer (NSCLC), however radiotherapy remains largely palliative due to radioresistance. Cancer stem cells (CSCs), as well epithelial-mesenchymal transition (EMT), may contribute drug and resistance mechanisms in solid tumors. Here we investigated the molecular phenotype of A549 H460 NSCLC that survived treatment IR (5Gy) are growing floating tumor spheres maintained a monolayer after...
Abstract Purpose: TMEM16A is a calcium-activated chloride channel that amplified in variety of cancers, including 30% head and neck squamous cell carcinomas (HNSCCs), raising the possibility an anti-apoptotic role malignant cells. This study investigated this using multimodal, translational investigation. Experimental Design: Combination (i) vitro HNSCC culture experiments assessing viability, apoptotic activation, protein expression (ii) vivo studies similar outcomes, (iii) molecular...
Squamous cell carcinoma of the head and neck (SCCHN) is a devastating disease that continues to have low cure rates despite recent advances in therapies. Cisplatin most used chemotherapy agent, treatment failure largely driven by resistance this drug. Amplification chromosomal band 11q13 occurs ∼30% SCCHN tumors. This region harbors ANO1 gene encodes TMEM16A ion channel, which responsible for calcium-activated chloride transport epithelial tissues. overexpression associated with cisplatin...
// Roberto Gomez-Casal 1, 2 , Michael W. Epperly 3 Hong Wang 4 David A. Proia 5 Joel S. Greenberger Vera Levina 2, 6 1 University of Pittsburgh Cancer Institute, Pittsburgh, PA, USA Department Medicine, Radiation Oncology, Biostatistics, Synta Pharmaceuticals Corp., Lexington, MA, Current address: Hillman Center, Correspondence to: Levina, e-mail: levinav@upmc.edu Keywords: lung adenocarcinoma, fractionated ionizing radiation, radioresistance mechanisms, DNA repair, heat shock protein-90...
The molecular chaperone HSP90 is involved in stabilization and function of multiple client proteins, many which represent important oncogenic drivers NSCLC. Utilization inhibitors as radiosensitizing agents a promising approach. antitumor activity ganetespib, inhibitor, was evaluated human lung adenocarcinoma (AC) cells for its ability to potentiate the effects IR treatment both vitro vivo. cytotoxic ganetespib included; G2/M cell cycle arrest, inhibition DNA repair, apoptosis induction,...
Cancer stem cells (CSC) typically over-express aldehyde dehydrogenase (ALDH). Thus, ALDHbright tumor represent targets for developing novel cancer prevention/treatment interventions. Loss of p53 function is a common genetic event during development wherein small molecular weight compounds (SMWC) that restore and reverse growth have been identified. Here, we focused on two widely studied SMWC, CP-31398 PRIMA-1, to target CSC in human breast, endometrial pancreas carcinoma cell lines...
Abstract Small cell lung cancer (SCLC) is the most aggressive and metastatic form of with a 5 year survival rate less than 7%. Typically, patients are diagnosed SCLC when tumor has already widely metastasized. Combination chemotherapy, generally platinum-based plus etoposide mainstay first-line treatments for SCLC. Despite being highly sensitive to therapy treatments, experience relapse resistant disease within 2 years die from systemic metastasis. Since there no approved therapies treatment...
<p>Supplemental packet contains supplemental figures for "TMEM16A/ANO1 overexpression inhibits apoptosis via down-regulation of Bim expression." It also uncropped blots corresponding to in the main and figures. Uncropped are labeled & grouped by figure panel which cropped version used.</p>
<div>Abstract<p><b>Purpose:</b> TMEM16A is a calcium-activated chloride channel that amplified in variety of cancers, including 30% head and neck squamous cell carcinomas (HNSCCs), raising the possibility an anti-apoptotic role malignant cells. This study investigated this using multimodal, translational investigation.</p><p><b>Experimental Design:</b> Combination (i) <i>in vitro</i> HNSCC culture experiments assessing viability,...
<div>Abstract<p><b>Purpose:</b> TMEM16A is a calcium-activated chloride channel that amplified in variety of cancers, including 30% head and neck squamous cell carcinomas (HNSCCs), raising the possibility an anti-apoptotic role malignant cells. This study investigated this using multimodal, translational investigation.</p><p><b>Experimental Design:</b> Combination (i) <i>in vitro</i> HNSCC culture experiments assessing viability,...
<p>Supplemental packet contains supplemental figures for "TMEM16A/ANO1 overexpression inhibits apoptosis via down-regulation of Bim expression." It also uncropped blots corresponding to in the main and figures. Uncropped are labeled & grouped by figure panel which cropped version used.</p>
ABSTRACT The calcium-activated chloride channel TMEM16A is overexpressed in several tumors. This condition associated with a poor survival prognosis but highlights TMEM16A’s potential as biomarker and target for anti-cancer therapies. Numerous somatic mutations of have been reported; however, their molecular mechanism oncogenesis are unknown. Here, we investigate the function oncogenicity nine-point found human cancerous tissues (R451P, R455Q, M546I, R557W, F604L, D902N, K913E, D914H,...
Abstract Non-small cell lung cancer (NSCLC), the most common form of cancer, is treated with tumor resection and chemotherapy, ionizing radiation (IR) treatment chemotherapy used for patients unresectable tumors. Despite improvements in medical management, 5-year survival rates NSCLC remain low. Evidence suggests that resistance to radio- associated stem cells (CSCs), which are undifferentiated capacity self-renew differentiate, restoring population following treatment. However, mechanisms...