A5032981031- PI3K/AKT/mTOR signaling in cancer
- Analytical Chemistry and Chromatography
- Mass Spectrometry Techniques and Applications
- Chronic Myeloid Leukemia Treatments
- Lymphoma Diagnosis and Treatment
- Vascular Tumors and Angiosarcomas
- RNA Research and Splicing
- Protein Tyrosine Phosphatases
- Cancer-related Molecular Pathways
- Guidance and Control Systems
- Chronic Lymphocytic Leukemia Research
- Metabolomics and Mass Spectrometry Studies
- Sarcoma Diagnosis and Treatment
- Advanced Chemical Sensor Technologies
- Bioactive Compounds and Antitumor Agents
- Cytokine Signaling Pathways and Interactions
- Liver Disease Diagnosis and Treatment
- Innovative Microfluidic and Catalytic Techniques Innovation
- Nonlinear Dynamics and Pattern Formation
- Hepatocellular Carcinoma Treatment and Prognosis
- Vehicle Dynamics and Control Systems
- Military Defense Systems Analysis
- Water Quality Monitoring Technologies
- Electron Spin Resonance Studies
- Dynamics and Control of Mechanical Systems
Hangzhou Medical College
2025
Harbin Institute of Technology
2023-2024
Purdue University West Lafayette
2017-2023
Changjiang Institute of Survey, Planning, Design and Research
2021
Anhui University
2014
Abstract Aim This study investigated the role of RAP1B in hepatic lipid metabolism and its implications obesity associated metabolic disorders, focusing on molecular mechanisms through which influences accumulation, inflammation oxidative stress liver tissues hepatocyte cell lines. Materials Methods Liver‐specific RAP1B‐knockout (LKO) overexpression (OE) mice were generated fed a high‐fat diet for 18 weeks to evaluate systemic changes. Comprehensive phenotyping included measurements body...
An integrated system utilizing mass spectrometry for reaction screening, continuous-flow optimization, and continuous crystallization of diphenhydramine has been developed.
Biothreat detection has continued to gain attention. Samples suspected fall into any of the CDC's biothreat categories require identification by processes that specialized expertise and facilities. Recent developments in analytical instrumentation machine learning algorithms offer rapid accurate classification Gram-positive Gram-negative bacterial species. This is achieved analyzing negative ions generated from cell extracts with a modified linear quadrupole ion-trap mass spectrometer fitted...
Advances in ambient ionization techniques have facilitated the direct analysis of complex mixtures without sample preparation. Significant attention has been given to innovating methods so that multiple options are now available, allowing for ready selection best particular analyte classes. These commonly implemented on benchtop systems, but their potential application with miniature mass spectrometers situ measurements is even more powerful. applications require be paid tailoring...
We demonstrate proof-of-concept for point-of-care assessment of long-term alcohol consumption by measuring phosphatidylethanol in blood/dried blood spots with nano-electrospray ionization and MS/MS using a miniature mass spectrometer.
Abstract The phosphatases of regenerating liver (PRL) are oncogenic when overexpressed. We previously found that PRL2 deletion increases PTEN, decreases Akt activity, and suppresses tumor development in a partial Pten-deficient mouse model. current study aims to further establish the mechanism PTEN regulation by expand therapeutic potential for augmentation mediated inhibition cancers initiated without alteration. TP53 gene is most mutated suppressor human cancers, heterozygous or complete...
Abstract Complex mixtures are typically analyzed by chromatographic separation followed mass spectrometry. Two‐dimensional tandem spectrometry (2D MS/MS) may eliminate the need for and therefore significantly reduce analysis time while expanding information space recording intensities of all fragment ions generated from trapped precursor ions. Additional chemical specificity can be provided using derivatization. In this study phenolic compounds identified after triazole click derivatization...
<p>Prl2 deletion inhibits tumor cell proliferations</p>
<p><i>Prl2</i> deletion enhances survival and hinders tumor growth rate in tamoxifen-inducible <i>Tp53<sup>−</sup><sup>/</sup><sup>−</sup></i> mouse but does not affect initiation. <b>A,</b> PCR genotyping to identify different genotypes the study. <b>B,</b> Kaplan–Meier plot for thymic lymphoma-free <i>Tp53<sup>−</sup><sup>/</sup><sup>−</sup> n</i> = 43...
<p>Experiemental design for tamoxifen inducible Tp53 deletion w/o Prl2 deletion</p>
<p><i>Prl2</i> deletion upregulates PTEN to inhibit Akt signaling and hinder tumor cell proliferation in inducible <i>Tp53<sup>−</sup><sup>/</sup><sup>−</sup></i> mice. <b>A,</b> Representative Western blot analysis from <i>Tp53<sup>−</sup><sup>/</sup><sup>−</sup></i>;...
<p><i>Prl2</i> deletion enhances survival in constitutive <i>Tp53</i> deficiency mouse models. <b>A,</b> Mating strategy for the generation of <i>Tp53</i>-deficient mice with or without <i>Prl2</i> deletion. <b>B,</b> PCR genotyping to identify different genotypes study. Kaplan–Meier plot overall <i>Tp53-H</i> (<b>C</b>) and <i>Tp53-KO</i> (<b>D</b>) backgrounds...
<p><i>Prl2</i> deletion upregulates PTEN to inhibit Akt signaling and hinder tumor cell proliferation in inducible <i>Tp53<sup>−</sup><sup>/</sup><sup>−</sup></i> mice. <b>A,</b> Representative Western blot analysis from <i>Tp53<sup>−</sup><sup>/</sup><sup>−</sup></i>;...
<p>Prl2 deletion does not affect Erk activation in inducible Tp53-/- mice derived thymic lymphomas</p>
<p><i>Prl2</i> deletion inhibits tumor cell proliferation but not apoptosis in tamoxifen-inducible <i>Tp53<sup>−</sup><sup>/</sup><sup>−</sup></i> mice. <b>A,</b> Representative Western blot analysis for marker PCNA and markers c-PARP c-caspase 3 from thymic lymphoma samples obtained 12 weeks after tamoxifen treatment. <b>B,</b> Dot plots quantification of relative to actin total protein control...
<p>Prl2 deletion upregulates PTEN to inhibit Akt signaling and hinder tumor cell proliferation in Tp53 heterozygous mice</p>
<p>PRL2 expression inversely correlates with PTEN and survival in human patients sarcoma. <b>A,</b> Scatter plot of PRL2 mRNA data showing the increased TCGA SARC TP53 mutations. Patient samples were divided into WT group (<i>n</i> = 159) mutated 82) based on mutation status, level two groups plotted. <b>B,</b> RPPA protein decreased 122) 67) <b>C,</b> reverse correlation between levels SARC. low 35) high 33) level, then...
<p>Loss of PRL1 or PRL3 does not improve tumor-free survival Tp53-null mice</p>