A5034407476- Entomopathogenic Microorganisms in Pest Control
- Insect Resistance and Genetics
- Nematode management and characterization studies
- Insect symbiosis and bacterial influences
- Fungal Biology and Applications
- Animal Genetics and Reproduction
- Mycorrhizal Fungi and Plant Interactions
- CRISPR and Genetic Engineering
- Viral Infectious Diseases and Gene Expression in Insects
- Studies on Chitinases and Chitosanases
- Ubiquitin and proteasome pathways
- Fungal and yeast genetics research
- Insect and Arachnid Ecology and Behavior
- RNA and protein synthesis mechanisms
- Cancer-related gene regulation
- Bacterial Genetics and Biotechnology
- Aquaculture disease management and microbiota
- Enzyme Structure and Function
- Cancer-related Molecular Pathways
- Molecular Biology Techniques and Applications
- MicroRNA in disease regulation
- Bacteriophages and microbial interactions
- Wnt/β-catenin signaling in development and cancer
- Cancer-related molecular mechanisms research
- Parasite Biology and Host Interactions
Chongqing University
2016-2025
Chongqing Science and Technology Commission
2012-2025
Yangzhou University
2017-2024
Taizhou People's Hospital
2020-2023
Nantong University
2020-2023
Nanjing Medical University
2023
UConn Health
2012-2019
Zhejiang Cancer Hospital
2019
Fudan University Shanghai Cancer Center
2019
University of Aberdeen
2017
Metarhizium spp. are being used as environmentally friendly alternatives to chemical insecticides, model systems for studying insect-fungus interactions, and a resource of genes biotechnology. We present comparative analysis the genome sequences broad-spectrum insect pathogen anisopliae acridid-specific M. acridum. Whole-genome analyses indicate that structures these two species highly syntenic suggest genus evolved from plant endophytes or pathogens. Both acridum have strikingly larger...
ABSTRACT Entomopathogenic fungi can produce a series of chitinases, some which act synergistically with proteases to degrade insect cuticle. However, chitinase involvement in fungus pathogenesis has not been fully characterized. In this paper, an endochitinase, Bbchit1, was purified homogeneity from liquid cultures Beauveria bassiana grown medium containing colloidal chitin. Bbchit1 had molecular mass about 33 kDa and pI 5.4. Based on the N-terminal amino acid sequence, gene, , its upstream...
ABSTRACT Entomopathogenic fungi are currently being used for the control of several insect pests as alternatives or supplements to chemical insecticides. Improvements in virulence and speed kill can be achieved by understanding mechanisms fungal pathogenesis genetically modifying targeted genes, thus improving commercial efficacy these biocontrol agents. fungi, such Beauveria bassiana , penetrate cuticle utilizing a plethora hydrolytic enzymes, including chitinases, which important factors....
Fungal insect pathogens have evolved diverse mechanisms to evade host immune recognition and defense responses. However, identification of fungal factors involved in evasion during cuticular penetration subsequent hemocoel colonization remains limited. Here, we report that the entomopathogenic fungus Beauveria bassiana expresses an endo-β-1,3-glucanase (BbEng1) functions helping cells recognition/ BbEng1 was specifically expressed infection, response cuticle hemolymph, presence osmotic or...
Chitin is an important component of the fungal cell wall with a family chitin synthases mediating its synthesis. Here, we report on genetic characterization full suite seven (MaChsI-VII) identified in insect pathogenic fungus, Metarhizium acridum. Aberrant distribution was most evident targeted gene knockouts MaChsV and MaChsVII. Mutants MaChsI, MaChsIII, MaChsIV showed delayed conidial germination, whereas ΔMaChsII ΔMaChsV mutants germinated more rapidly when compared to wild-type parent....
Fungal biocontrol agents have great potential in integrated pest management. However, poor efficacy and sensitivity to various adverse factors hampered their wide application. In eukaryotic cells, Hog1 kinase plays a critical role stress responses. this study, MaHog1 (GenBank accession no. EFY85878), encoding member of the Hog1/Sty1/p38 mitogen-activated protein family Metarhizium (Me.) acridum, was identified. Targeted gene disruption used analyse virulence tolerance factors. Mutants with...
The Gti1/Pac2 protein family, which is highly conserved across fungi, pivotal in processes such as fungal development, spore formation, export, toxin production, and virulence. Despite its importance, the precise functions of Pac2 within entomopathogenic fungi have yet to be fully understood. In our study, MaPac2 gene from M. acridum was identified, were explored. Studying domain showed that comprises 422 amino acids with a characteristic family (Pfam09729). Additionally, predicted an...
Nitrogen is an essential nutrient that frequently determines the growth rate of fungi. Nitrate transporter proteins (Nrts) play a crucial role in cellular absorption nitrate from environment. Entomopathogenic fungi (EPF) have shown their potential biological control pests. Thus, comprehending mechanisms govern pathogenicity and stress tolerance EPF helpful improving effectiveness practical application these fungal biocontrol agents. In this study, we utilized homologous recombination to...
Significance The cyclin D1 proto-oncoprotein is a crucial regulator of cell cycle progression, and excessive expression and/or activity hallmark many human cancers. SCF FBXO31 ubiquitin ligase complex responsible for targeting ubiquitination degradation, thereby regulating its turnover after DNA damage. In this work, we report the structural, biophysical, functional characterization interaction between Skp1–FBXO31. We find that F-box protein targets phosphorylation-independent by recognizing...
The SleB protein is one of two redundant cortex-lytic enzymes (CLEs) that initiate the degradation cortex peptidoglycan (PG), a process essential for germination spores Bacillus species, including anthracis. has been characterized as soluble lytic transglycosylase specifically recognizes spore PG and catalyzes cleavage glycosidic bonds between N-acetylmuramic acid (NAM) N-acetylglucosamine residues with concomitant formation 1,6-anhydro bond in NAM residue. We found like full-length cereus...
Beauveria bassiana is an important entomopathogenic fungus widely used as a biological agent to control insect pests. A gene (B. JEN1 [BbJEN1]) homologous encoding carboxylate transporter in Saccharomyces cerevisiae was identified B. transfer DNA (T-DNA) insertional mutant. Disruption of the decreased contents hyphae, while increasing conidial yield. However, overexpression this resulted significant increases carboxylates and BbJEN1 strongly induced by cuticles highly expressed hyphae...
Phosphomannosylation is a modification of cell wall proteins that occurs in some species yeast-like organisms, including the human pathogen Candida albicans. These modified mannans confer negative charge to wall, which important for interactions with phagocytic cells immune systems and cationic antimicrobial peptides. In Saccharomyces cerevisiae, synthesis phosphomannan relies on two enzymes, phosphomannosyltransferase Ktr6 its positive regulator Mnn4. However, C. albicans, at least three...
Abstract BACKGROUND The fungal genera Metarhizium contain many important multiple species that are used as biocontrol agents and model organisms for exploring insect–fungal interactions. spp. exhibit different traits of pathogenicity, suggesting the pathogenesis can be quite distinctive. However, underlying differences in their remain poorly understood. RESULTS Pathogenicity analysis showed anisopliae (strain CQMa421) displayed higher virulence against oriental migratory locusts, Locusta...