A5048837960- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Immune Cell Function and Interaction
- Vasculitis and related conditions
- T-cell and B-cell Immunology
- Immune Response and Inflammation
- Immunotherapy and Immune Responses
- Atherosclerosis and Cardiovascular Diseases
- Cytomegalovirus and herpesvirus research
- Cytokine Signaling Pathways and Interactions
- DNA and Nucleic Acid Chemistry
- Cell Adhesion Molecules Research
- Single-cell and spatial transcriptomics
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- IL-33, ST2, and ILC Pathways
- Protein Tyrosine Phosphatases
- Renal Diseases and Glomerulopathies
- Nanoplatforms for cancer theranostics
- Plasmonic and Surface Plasmon Research
- Galectins and Cancer Biology
- Extracellular vesicles in disease
- Bacteriophages and microbial interactions
- NF-κB Signaling Pathways
- Viral Infections and Immunology Research
- CAR-T cell therapy research
Center for Rheumatology
2024
Stanford University
2019-2023
Mayo Clinic
2022-2023
Mayo Clinic in Arizona
2022
Kyoto Pharmaceutical University
2015-2021
Kyoto University
2015-2021
Kumamoto University
2019-2020
DNA dendrimers consisting of several branched units connected to each other using ligase were quite effective for the delivery immunostimulatory CpG immune cells. Therapeutic application such dendrimers, however, is hampered by use ligase. Here, we report that self-assembling with high potency can be prepared without ligases. Annealing elongated adhesive ends resulted in formation dendrimers. Atomic force microscopy revealed preparations dendritic structures as designed. The cellular uptake...
Autoimmune vasculitis of the medium and large elastic arteries can cause blindness, stroke, aortic arch syndrome, aneurysm. The disease is often refractory to immunosuppressive therapy progresses over decades as smoldering aortitis. How granulomatous infiltrates in vessel wall are maintained how tissue-infiltrating T cells macrophages replenished unknown. Single-cell whole-tissue transcriptomic studies immune cell populations vasculitic identified a CD4 + population with stem cell–like...
Loss of function inhibitory immune checkpoints, unleashing pathogenic responses, is a potential risk factor for autoimmune disease. Here, we report that patients with the vasculitis giant cell arteritis (GCA) have defective CD155-CD96 checkpoint. Macrophages from GCA retain checkpoint ligand CD155 in endoplasmic reticulum (ER) and fail to bring it surface. CD155low antigen-presenting cells induce expansion CD4+CD96+ T cells, which become tissue invasive, accumulate blood vessel wall, release...
Objective: Granulomatosis with polyangiitis (GPA) is a multi-organ vasculitic syndrome typically associated neutrophil extracellular trap (NET) formation and aggressive tissue inflammation disruption. Head neck (H&N) GPA manifestations include septal perforations, saddle-nose deformities, bony erosions of the orbital sinus walls, middle ear damage epiglottitis, indicative bone, cartilage connective destruction. Whether H&N-centric lesions engage disease pathways distinctive from ischemic in...
The human adult immune system maintains normal T cell counts and compensates for loss throughout life, mainly through peripheral homeostatic proliferation after the ability of thymus to generate new cells has rapidly declined at adolescence. This process is driven by STAT5-activating cytokines, most importantly IL-7, very effective in maintaining a large naive CD4+ compartment into older age. Here, we describe that undergo adaptations optimize IL-7 responses upregulating guanine-nucleotide...
Using DNA nanostructures with almost identical molecular weight and structural flexibility, this work clearly showed that compactly packaged high density are suitable for the delivery to immune cells.
Nanosized DNA assemblies are useful for delivering immunostimulatory cytosine–phosphate–guanine (CpG) to immune cells, but little is known about the optimal structure such delivery. In this study, we designed three different nanostructures using four 55-mer oligodeoxynucleotides (ODNs), that is, tetrapod-like structured (tetrapodna), tetrahedral (tetrahedron), and tetragonal (tetragon), compared their potencies. Electrophoresis showed tetrapodna was obtained with high yield purity, whereas...
Abstract We used human Toll-like receptor 9 (hTLR9)-expressing HEK-Blue hTLR9 cells, which release secreted embryonic alkaline phosphatase (SEAP) upon response to CpG DNA, evaluate the immunological properties of nucleic acid drug candidates. Our preliminary studies showed that phosphodiester DNA hardly induced any SEAP secretion in cells. In current study, therefore, we developed cells transduced with macrophage scavenger receptor-1 (hMSR1), a cell-surface receptor, and determined whether...
Rectangular DNA with many staples, a more rigid structure than the others, efficiently interacted immune cells.