We have developed a new technology for producing three-dimensional (3D) biological structures composed of living cells and hydrogel in vitro, via the direct accurate printing with an inkjet system. Various were constructed our custom-made printer, which we termed 3D bioprinter. In present study, used alginate that was obtained through reaction sodium solution calcium chloride solution. For construction gel structure, ejected from nozzle (SEA-Jet™, Seiko Epson Corp., Suwa, Japan) mixed...

The next step beyond conventional scaffold-based tissue engineering is cell-based direct biofabrication techniques. In industrial processes, various three-dimensional (3D) prototype models have been fabricated using several different rapid prototyping methods, such as stereo-lithography, 3D printing and laser sintering, well others, in which a variety of chemical materials are utilized. However, with biofabrication, only biocompatible can be used, the manufacturing process must performed...

Tissue engineering has been developed with the ultimate aim of manufacturing human organs, but success limited to only thin tissues and no significant structures. In order construct more complicated tissues, we have a three-dimensional (3D) fabrication technology in which 3D structures are directly built up by layer-by-layer printing living cells several tissue components. We custom-made inkjet printer specially designed for this purpose. Recently, was improved, on-demand mode installed...

Hydroxyapatite (HAP) is a main mineral constituent of bone and tooth has an outstanding biocompatibility. HAP possible sorbent for heavy metals in wastewater due to its high adsorption capacity low water solubility. We developed removal system 90Sr from aqueous solution by column procedure. More than 90 % was adsorbed removed the containing solution. Divalent cations, Ca2+, had little effect on up concentration 1 mmol L-1. This clearly indicates that technique advantageous with respect...

The utility of hypromellose acetate succinate (HPMCAS), a cellulosic enteric coating agent, as carrier in solid dispersion nifedipine (NP) was evaluated comparison with other polymers, including (HPMC), phthalate (HPMCP), methacrylic acid ethyl acrylate copolymer (MAEA), and povidone (PVP). An X-ray diffraction study showed that the minimum amount HPMCAS required to make drug completely amorphous same less than dispersions using non-cellulosic polymers. Hypromellose highest dissolution level...

Ink jet technology has advantages such as high-resolution and a multicolor printing capability good potential for computer-based three-dimensional (3D) fabrication. The authors have developed an ink 3D bioprinter to manufacture biologically viable structures using living cells. They effective method the fabrication of hydrogel by with liquid aqueous gelating medium, which is essential in fabricating In present study, they evaluated feasibility approach digital biofabrication, analyzing...

The enhanced release of reactive oxygen species from activated neutrophils plays important role in the pathogenesis inflammatory bowel disease. We previously reported that radon inhalation activates antioxidative functions various organs mice. In this study, we examined protective effects on dextran sulfate sodium- (DSS) induced colitis mice which were subjected to DSS for 7 days. Mice continuously treated with air only (sham) or at a concentration 2000 Bq/m³ day before administration end...

We examined dose–dependent or dose rate–dependent changes of superoxide dismutase (SOD) activity using a new large-scale facility for exposing small animals to radon. Mice were exposed radon at concentration 250, 500, 1000, 2000, 4000 Bq/m3 0.5, 1, 2, 4, 8 days. When mice 2000 day•Bq/m3, activation SOD activities in plasma, liver, pancreas, heart, thymus, and kidney showed dose–rate effects. Our results also suggested that continuous exposure increased activity, but transiently returned...

We have previously reported that radon inhalation activates anti-oxidative functions and inhibits carbon tetrachloride (CCl4)-induced hepatopathy. It has also been antioxidant vitamins can inhibit CCl4-induced In the current study, we examined comparative efficacy of treatment with radon, ascorbic acid α-tocopherol on Mice were subjected to intraperitoneal injection CCl4 after inhaling approximately 1000 or 2000 Bq/m3 for 24 h, immediately (100, 300, 500 mg/kg bodyweight) bodyweight)....

3D micro-fabrication technique is one of the essential technologies in manufacturing complicated biological tissues by science and technology. We have developed "inkjet biofabrication approach" as a technology to build up designed with micro- macro-structures handling different multi-types living cells. In our using inkjet printing gel precursor reactant, hydrogel structures can be fabricated. As biocompatible hydrogel, we use alginate which protects cells from drying keep liquid media. With...

Since the 2011 nuclear accident in Fukushima, effects of low-dose irradiation, especially internal exposure, are at forefront everyone's attention. However, radiation induced various stimulating such as activation antioxidative and immune functions. In this study, we attempted to evaluate quantitative activities kidney by radon inhalation on carbon tetrachloride (CCl4)-induced renal damage. Mice were subjected intraperitoneal (i.p.) injection CCl4 after inhaling approximately 1000 or 2000...

Abstract Background Radon therapy is clinically useful for the treatment of pain‐related diseases. However, there have been no studies regarding effects radon inhalation on neuropathic pain. In this study, we aimed to determine whether actually induced a remission pain and improved quality life. Methods First, investigated antinociceptive in chronic constriction injury ( CCI ) model We evaluated behaviour mice before after surgery, using von F rey test. Pretreated received surgery...

We previously reported that radon inhalation activates antioxidative functions in the liver and inhibits carbon tetrachloride-induced hepatopathy mice. In addition, it has been reactive oxygen species contribute to alcohol-induced hepatopathy. this study, we examined inhibitory effects of on acute C57BL/6J mice were subjected intraperitoneal injection 50% alcohol (5 g/kg bodyweight) after inhaling approximately 4000 Bq/m 3 for 24 h. Alcohol administration significantly increased activities...

We examined the protective effect of radon inhalation on streptozotocin (STZ)-induced type-1 diabetes in mice. Mice inhaled at concentrations 1000, 2500, and 5500 Bq/m3 for 24 hours before STZ administration. administration induced characteristics such as hyperglycemia hypoinsulinemia; however, doses 1000 significantly suppressed elevation blood glucose diabetic Serum insulin was higher mice pre-treated with a dose than treated sham. In addition, superoxide dismutase activities total...

The intestinal tract is a typical radiosensitive tissue, and radiation rectal injury severe side effect that limits the prescribed dose in radiotherapy of abdominal pelvic region. Understanding post-irradiation kinetics Lgr5-positive stem cells crucial comprehending this adverse process. In study, we utilized Lgr5-EGFP knock-in mice expressing EGFP LGR5 antibody fluorescence staining wild-type mice. At state injury, qPCR analysis showed significant decrease expression level Lgr5 epithelial...