A5070313195- Photodynamic Therapy Research Studies
- bioluminescence and chemiluminescence research
- Nanoplatforms for cancer theranostics
- Laser Applications in Dentistry and Medicine
- Bacterial biofilms and quorum sensing
- Ocular Infections and Treatments
- Photoreceptor and optogenetics research
- Antifungal resistance and susceptibility
- Reproductive tract infections research
- Biosensors and Analytical Detection
- Wound Healing and Treatments
- Medical and Biological Ozone Research
- Essential Oils and Antimicrobial Activity
- Antibiotic Resistance in Bacteria
- Dental Research and COVID-19
- Dermatologic Treatments and Research
- Bacterial Infections and Vaccines
- Antibiotic Use and Resistance
- Vibrio bacteria research studies
- Urinary Tract Infections Management
- Smart Agriculture and AI
- Microbial Inactivation Methods
- Orthopedic Infections and Treatments
- Connexins and lens biology
- Infection Control and Ventilation
Massachusetts General Hospital
2015-2024
Harvard University
2015-2024
Northeast Forestry University
2021-2022
Harvard–MIT Division of Health Sciences and Technology
2011
Rice University
2003
Background. Biofilms affect >80% bacterial infections in human and are usually difficult to eradicate because of their inherent drug resistance. Methods. We investigated the effectiveness antimicrobial blue light (aBL) (wavelength, 415 nm) for inactivating Acinetobacter baumannii or Pseudomonas aeruginosa biofilms 96-well microplates infected mouse burn wounds. Results. In vitro, microplates, exposure 24-hour-old 72-hour-old A. 432 J/cm2 aBL resulted inactivation 3.59 log10 3.18...
Development of non-antibiotic alternatives to treat infections caused by multidrug-resistant (MDR) microbes represents one the top priorities in healthcare and community settings, especially care combat trauma-associated wound infections. Here, we investigate efficacy oregano oil against pathogenic bacteria including MDR isolates from casualties vitro a mouse burn model. Oregano showed significant anti-bacterial activity 11 clinical four Acinetobacter baumannii , three Pseudomonas aeruginosa...
Bacterial skin and soft tissue infections (SSTI) affect millions of individuals annually in the United States. Treatment SSTI has been significantly complicated by increasing emergence community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) strains. The objective this study was to demonstrate efficacy blue light (415 ± 10 nm) therapy for eliminating CA-MRSA abrasions mice.The susceptibilities a strain (USA300LAC) human keratinocytes (HaCaT) inactivation were compared vitro...
Fungal infections are a common cause of morbidity, mortality and cost in critical care populations. The increasing emergence antimicrobial resistance necessitates the development new therapeutic approaches for fungal infections. In present study, we investigated effectiveness an innovative approach, blue light (aBL), inactivation Candida albicans vitro infected mouse burns. A bioluminescent strain C. was used. susceptibilities to aBL (415 nm) were compared between human keratinocytes....
Development of alternatives to antibiotics is one the top priorities in battle against multidrug-resistant (MDR) bacterial infections. Here, we report that two naturally occurring nonantibiotic modalities, blue light and phytochemical carvacrol, synergistically kill an array bacteria including their planktonic forms, mature biofilms, persisters, irrespective antibiotic susceptibility. Combination but not single treatment completely or substantially cured acute established biofilm-associated...
Antimicrobial blue light (aBL) offers efficacy and safety in treating infections. However, the bacterial targets for aBL are still poorly understood may be dependent on species. Here, we investigated biological of killing by (λ = 410 nm) three pathogens: Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa. Initially, evaluated kinetics bacteria exposed to used this information calculate lethal doses (LD) responsible 90 99.9% bacteria. We also quantified endogenous porphyrins...
Abstract Confronted with the severe situation that pace of resistance acquisition is faster than clinical introduction new antibiotics, health organizations are calling for effective approaches to combat methicillin‐resistant Staphylococcus aureus (MRSA) infections. Here, an approach treat MRSA through photolysis staphyloxanthin, antioxidant residing in microdomain S. membrane, reported. This photochemistry process uncovered transient absorption imaging and quantitated by spectroscopy, Raman...
Polymicrobial biofilms, in which mixed microbial species are present, play a significant role persistent infections. Furthermore, polymicrobial biofilms promote antibiotic resistance by allowing interspecies transfer of genes. In the present study, we investigated effectiveness antimicrobial blue light (aBL; 405 nm), an innovative non-antibiotic approach, for inactivation biofilms. Dual-species with Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus (MRSA) as well P....
Abstract Resolution is an active process that protects the host damage from inflammation responses induced by infections. Simultaneously resolving and eliminating pathogens may be effective to treat infectious diseases, but it required deliver therapeutics sites. Here, we proposed a strategy incorporate RvD1 antibiotic (ceftazidime) in human neutrophil-membrane derived nanovesicles can specifically target inflamed vasculature for treatment of lung infection caused P. aeruginosa . Using...
Antimicrobial blue light (aBL) has attracted increasing interest for its antimicrobial properties. However, the underlying bactericidal mechanism not yet been verified. One hypothesis is that aBL causes excitation of intracellular chromophores; leading to generation reactive oxygen species (ROS) and resultant oxidization various biomolecules. Thus, monitoring levels redox-sensitive biomolecules such as coproporphyrins, well singlet ROS may help uncover physiological changes induced by aid in...
The limited penetration depth of light in skin tissues is a practical bottleneck dermatologic applications light-induced therapies, including anti-microbial blue therapy and photodynamic cancer therapy.Here, we demonstrate novel device, termed optical microneedle array (OMNA), for percutaneous delivery.A prototype device with 11 by needles at spacing 1 mm length 1.6 was fabricated press-molding poly-(lactic acid) (PLA) polymers.The also incorporates matched microlens that focuses the through...
Biofilms cause more than 80% of infections in humans, including 90% all chronic wound and are extremely resistant to antimicrobials the immune system. The situation is exacerbated by fast spreading antimicrobial resistance, which has become one biggest threats current public health. There consequently a critical need for development alternative therapeutics. Antimicrobial blue light (aBL) light-based approach that exhibits intrinsic effect without involvement exogenous photosensitizers. In...
Despite decades of efforts, state-of-the-art synthetic burn dressings to treat partial-thickness burns are still far from ideal. Current adhere the wound and necessitate debridement. This work describes first "supramolecular hybrid hydrogel (SHH)" dressing that is biocompatible, self-healable, on-demand dissoluble for easy trauma-free removal, prepared by a simple, fast, scalable method. These SHHs leverage interactions custom-designed cationic copolymer via host-guest chemistry with...
Antimicrobial strategies for musculoskeletal infections are typically first developed with in vitro models. The In Vitro Section of the 2023 Orthopedic Research Society Musculoskeletal Infection international consensus meeting (ICM) probed our state knowledge systems respect to bacteria and biofilm phenotype, standards, activity, ability predict vivo efficacy. A subset ICM delegates performed systematic reviews on 15 questions made recommendations assessment level evidence that were then...
Antimicrobial resistance is a threat to public health that requires our immediate attention. With increasing numbers of microbes are becoming resistant routinely used antimicrobials, it vital we look other, non-traditional therapies for the treatment infections. blue light (aBL) an innovative approach has demonstrated efficacy inactivation array microbial pathogens. In present study, investigated potential development aBL in Gram-negative pathogenic bacteria by carrying out multiple...
Melasma on the face is difficult to treat and often refractory multiple treatment modalities.To investigate safety efficacy of fractional photothermolysis (FP) for melasma determine recurrence rates with this method.Eight female patients (Fitzpatrick skin type II-IV) clinically diagnosed were treated using FP (1,550 nm Fraxel SR laser). Two seven treatments performed at 3- 8-week intervals. Treatment levels ranged from 3 10, corresponding 9% 29% surface area coverage (8-10 passes per...
Antimicrobial resistance in Neisseria gonorrhoeae is a major issue of public health, and there critical need for the development new antigonococcal strategies. In this study, we investigated effectiveness antimicrobial blue light (aBL; wavelength, 405 nm), an innovative nonpharmacological approach, inactivation N. gonorrhoeae. Our findings indicated that aBL preferentially inactivated gonorrhoeae, including antibiotic-resistant strains, over human vaginal epithelial cells vitro. Furthermore,...
Background and Objectives The aim of this study was to investigate the efficacy, safety, mechanism action antimicrobial blue light (aBL) for inactivation Neisseria gonorrhoeae , etiological agent gonorrhea. Study Design/Materials Methods susceptibilities N. (ATCC 700825) in planktonic suspensions aBL at 405‐ 470‐nm wavelengths were compared. roles oxygen anti‐gonococcal activity studied by examining effects hypoxic condition (blowing N 2 ) on efficiency 405‐nm aBL. presence, identification,...