A5034206959- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Sperm and Testicular Function
- Mesenchymal stem cell research
- Chromosomal and Genetic Variations
- Tissue Engineering and Regenerative Medicine
- Speech Recognition and Synthesis
- Adsorption and biosorption for pollutant removal
- Speech and Audio Processing
- Viral Infectious Diseases and Gene Expression in Insects
- Congenital heart defects research
- Music and Audio Processing
- Bone Tissue Engineering Materials
- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- Phytochemistry and Bioactivity Studies
- Mass Spectrometry Techniques and Applications
- Extraction and Separation Processes
- Spatial Cognition and Navigation
- Bioactive Compounds and Antitumor Agents
- Interactive and Immersive Displays
- TiO2 Photocatalysis and Solar Cells
- Network Packet Processing and Optimization
- Wireless Communication Networks Research
National Taiwan University
2005-2024
National Taiwan University of Science and Technology
2019-2024
Tokushima University
2023-2024
Fu Jen Catholic University
2024
Institute of Medical Sciences
2024
Tokushima College of Technology
2023
Hunan University
2019
TaiGen Biotechnology (Taiwan)
2018
Institut Cellule Souche et Cerveau
2018
Inserm
2018
Engineering graphics courses are typically a requirement for engineering students around the world. Besides understanding and depicting graphic representation of objects, goal these is to provide with an relationship between three-dimensional (3D) objects their projections. However, in classroom, where time limited, it very difficult explain 3D geometry using only drawings on paper or at blackboard. The research presented herein aims develop two teaching aids; tangible model augmented...
In text, word2vec transforms each word into a fixed-size vector used as the basic component in applications of natural language processing. Given large collection unannotated audio, audio can also be trained an unsupervised way using sequence-to-sequence autoencoder (SA). These representations are shown to effectively describe sequential phonetic structures segments. this paper, we further extend research following two directions. First, disentangle information and speaker from SA...
ABSTRACT Chicken embryos are a powerful and widely used animal model in developmental biology studies. Since the development of CRISPR technology, gene-edited chickens have been generated by transferring primordial germ cells (PGCs) into recipients after genetic modifications. However, low inheritance caused competition between host transferred is common complication greatly reduces production efficiency. Here, we chicken, which can be ablated drug-dependent manner, as for PGC transfer. We...
This study aimed to isolate, culture, and characterize duck primordial germ cells (PGCs) compare these with chicken PGCs. We first cultured Muscovy (Cairina moschata) circulating PGCs gonadal (gPGCs) in the modified serum-containing medium used amplify gPGCs were found proliferate better serum-free chemically defined than medium. Thereafter, similarly isolated from 2 other breeds, Pekin (Anas platyrhynchos) hybrid mule (C. moschata × A. platyrhynchos), amplified for a limited period of time...
Scalable production of avian cell lines exhibits a valuable potential on therapeutic application by producing recombinant proteins and as the substrate for virus growth due to special glycosylation occurs in species. Chicken primordial germ cells (cPGCs), germinal pluripotent type, present ability self-renewal, an anchorage-independent be genetically modified. This type could interesting bioreactor system industrial purposes. study sought establish expandable culture with defined components...
Myocardial infarction (MI) is a leading cause of death worldwide, resulting in extensive loss cardiomyocytes and subsequent heart failure. Inducing cardiac differentiation stem cells potential approach for myocardial regeneration therapy to improve post-MI prognosis. Mesenchymal (MSCs) have several advantages, including immune privilege multipotent potential. This study aimed explore the feasibility chemically inducing human amniotic membrane MSCs (hAMSCs) differentiate into vitro. Human...
CdS/Titanate nanotubes (TNTs) were successfully synthesised by a simple, rapid, and reliable microwave hydrothermal method. The CdS nanoparticles using 140-W irradiation power at 423 K photodegraded 26% ammonia in water, while the photocatalytic efficiency increased to 52.3% CdS/TNTs composites. results indicated that photocatalysts possess improved activity than of or TNTs materials alone.
Food shortages due to population growth and climate change are expected occur in the near future as a problem that urgently requires solutions. Conventional breeding techniques, notably crossbreeding mutation breeding, known for being inefficient time-consuming obtaining seeds seedlings with desired traits. Thus, there is an urgent need novel methods efficient plant breeding. Breeding by genome editing receiving substantial attention because it can efficiently modify target gene obtain...
BackgroundThe aim of this study was to investigate whether quantitative changes in lymphocyte subsets and gene expression peripheral blood (PB) cells are related the clinical manifestations pathogenesis lupus nephritis (LN).MethodsWe enrolled 95 pediatric-onset SLE patients with renal involvement who presented 450 episodes suspicious for LN flare. Percentages at each episode were determined. We stratified 55 as high or low subset group according median percentage association eGFR (ΔeGFR)...
The in vitro sperm quality parameters (motility, M; viability, V; normal morphology, NM; plasma membrane integrity, PMI; mitochondrial function, MF) Muscovy drakes (Cairina moschata) were evaluated by using microscopy and flow cytometry, the correlation among results of artificial insemination was also assessed present study. M, V NM detected phase contrast assisted with eosin-nigrosin staining, PMI MF cytometry within appropriate fluorescence staining (SYBR-14/PI R123/PI, respectively)....
Background Cells, scaffolds, and factors are the triad of regenerative engineering; however, it is difficult to distinguish whether cells in construct from seeded or host via blood supply. We performed a novel vivo study transplant enhanced green fluorescent pig mesenchymal stem (EGFP-pMSCs) into calvarial defect DsRed pigs. The cell distribution proportion were distinguished by different colors through whole period. Method/Results Eight adult domestic Ds-Red pigs treated with five...
The PIWI/piRNA pathway is a conserved machinery important for germ cell development and fertility. This piRNA-guided molecular best known repressing derepressed transposable elements (TE) during epigenomic reprogramming. extent to which piRNAs are involved in modulating transcripts beyond TEs still need be clarified, it may stage-dependent event. We chose chicken germline as study model because of the significantly lower TE complexity genome compared mammalian species. generated...
Abstract Silane moisture‐cured metallocene‐catalyzed polyethylene (mPE) to form an elastomer has been prepared. Metallocene polyethylenes with two different levels of comonomer contents were grafted various amounts vinyltriethoxy silane. “Threshold” fracture energy is roughly proportional the reciprocal square root Young's modulus. By relating tensile strength tear strength, corrected average depth flaw in range 40.5 ± 11.0 μm, which successfully confirms extension conventional elastomeric...
This study describes a matrix-assisted laser-desorption-ionization (MALDI) mass spectrometry for rapid screening of 12 diuretics in spiked urine. C60 is used as the matrix MALDI. Diuretics are directly analyzed by C60-MALDI without previous derivatization. The fact that most diuretic molecules contain sulfate groups accounts why anions can be easily desorbed and ionized Using advantageous because low background range on negative MALDI spectrum. A clear window between m/z 200 600 ion mode...
Abstract Scalable production of avian suspension cell exhibits a valuable potential on therapeutic application by producing recombinant protein and as the substrate for virus growth. This study sought to establish system with chemically defined components three-dimensional (3D) culture chicken primordial germ cells (cPGCs), pluripotent type. cPGCs were cultured in medium supplemented functional polymer FP003. Viscoelasticity was low this medium, did not sediment,and consequently their...
A two-step procedure, combining a SmI
We propose a multiuser detection scheme for channelized fast frequency hopping with binary shift keying in uncoordinated, narrow-band systems. "Multiuser" here means uncoordinated users and the desired user. This consists of channel state detector detector. Both detectors are Gaussian approximated energy combiners sub-optimal maximum posteriori probability sense. In symbol interval, first detects existence signals each chip, then properly combines received chip energies to make decision....