A5015069479- Organ Transplantation Techniques and Outcomes
- Liver Disease and Transplantation
- Liver Disease Diagnosis and Treatment
- RNA Interference and Gene Delivery
- Liver physiology and pathology
- Endoplasmic Reticulum Stress and Disease
- Advanced biosensing and bioanalysis techniques
- Autophagy in Disease and Therapy
- Animal Virus Infections Studies
- Renal Transplantation Outcomes and Treatments
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Fuel Cells and Related Materials
- Drug-Induced Hepatotoxicity and Protection
- Macrophage Migration Inhibitory Factor
- Silymarin and Mushroom Poisoning
- Antibiotics Pharmacokinetics and Efficacy
- Tuberculosis Research and Epidemiology
- Sepsis Diagnosis and Treatment
- Biochemical effects in animals
- RNA and protein synthesis mechanisms
- Cardiac Ischemia and Reperfusion
- Advanced Glycation End Products research
- Alcohol Consumption and Health Effects
- Vascular anomalies and interventions
- Poisoning and overdose treatments
Certe
2025
Université Paris-Saclay
2021-2022
Université Paris-Sud
2016-2020
Inserm
2016-2020
Hôpital Paul-Brousse
2016-2018
Assistance Publique – Hôpitaux de Paris
2016-2018
Consejo Superior de Investigaciones Científicas
2017
University of Monastir
2017
Hospital Clínico Universitario Lozano Blesa
2016-2017
Universitat de Barcelona
2016-2017
<title>Abstract</title> mRNA vaccines have shown great efficacy against SARS-CoV-2, yet challenges remain in optimizing vaccine components to achieve enhanced immune response and stability. In this study, we developed CPVax-CoV, a new lyophilized that features novel thiolactone-based ionizable lipids newly designed untranslated regions (UTRs) for expression. Incorporation of these optimized into our candidate CPVax-CoV significantly improved responses mice compared commercially available...
Ionizable lipids are essential components of lipid nanoparticles (LNPs) for efficient mRNA delivery. However, designing them high protein expression, endosomal escape, and organ targeting is challenging due to complex structure-activity relationships. Here, we present a high-throughput platform screening ionizable using two-step, scalable, one-pot reaction. This enabled the synthesis vivo 91 new lipids, followed by study, leading development CP-LC-0729, which significantly surpasses MC3...
Improving the protection of marginal liver grafts during static cold storage is a major hurdle to increase donor pool organs. The endothelium glycocalyx quality preservation influences future inflammatory and oxidative responses. One cellular pathway responsible for formation nitric oxide by endothelial cells dependent on stimulation proteoglycans present in glycocalyx. We investigated impact fatty preserved different solutions endothelium-mediated production NO. Zucker rat livers were 24 h...
Institut George Lopez-1 (IGL-1) and Histidine-tryptophan-ketoglutarate (HTK) solutions are proposed as alternatives to UW (gold standard) in liver preservation. Their composition differs terms of the presence/absence oncotic agents such HES or PEG, is decisive for graft conservation before transplantation. This especially so when fatty (steatotic) livers used since these grafts more vulnerable ischemia insult during conservation. determines extent subsequent reperfusion injury after Aldehyde...
ABSTRACT Ionizable lipids are an essential component of lipid nanoparticles (LNPs) for efficient mRNA delivery. However, optimizing their chemical structures high protein expression, endosomal escape, and selective organ targeting remains challenging due to complex structure-activity relationships multistep synthesis. In this study, we introduce a rapid, high-throughput platform screening ionizable using two-step, scalable synthesis involving one-pot 3-component click-like reaction. This...
Circular RNA (circRNA) has gained significant attention as a potential therapeutic tool due to its remarkable stability and resistance degradation by exonucleases. However, scalable efficient methods for purification delivery remain critical challenges that must be addressed. In this study, we developed evaluated an optimized affinity chromatography method using Oligo(dT) columns the of circRNA, achieving high yield purity comparing with high-performance liquid chromatography. Additionally,...
Investigations were carried out to determine the antiulcer effects of silymarin, hepatoprotective principle Silybum marianum L. Gaertn., in gastric injury induced by ischemia-reperfusion and its on mucosal myeloperoxidase activity, an index polymorphonuclear leukocyte infiltration, after rats. These results compared with those from rats that received allopurinol, inhibitor xanthine oxidase made neutropenic prior administration dexamethasone methotrexate. Pretreatment silymarin prevented...
We investigated the involvement of glycogen synthase kinase-3β (GSK3β) and voltage-dependent anion channel (VDAC) in livers subjected to cold ischemia-reperfusion injury (I/R) associated with orthotopic liver transplantation (OLT). Rat were preserved University Wisconsin (UW) Institute Georges Lopez (IGL-1) solution, latter enriched or not trimetazidine, then OLT. Transaminase (ALT) HMGB1 protein levels, glutamate dehydrogenase (GLDH), oxidative stress (MDA) measured. The AKT kinase its...
The 26S proteasome is the central proteolytic machinery of ubiquitin system (UPS), which involved in degradation ubiquitinated protein substrates. Recently, UPS inhibition has been shown to be a key factor fatty liver graft preservation during organ cold storage using University Wisconsin solution (UW) and Institute Georges Lopez (IGL-1) solutions. However, merits IGL-1 histidine-tryptophan-ketoglutarate (HTK) solutions for have not compared. Fatty grafts from obese Zücker rats were...
Institute Goeorges Lopez 1 (IGL-1) and Histidine-Tryptophan-Ketoglutarate (HTK) preservation solutions are regularly used in clinical for liver transplantation besides University of Wisconsin (UW) solution Celsior. Several trials experimental works have been carried out comparing all the solutions, however comparative IGL-1 HTK appraisals poor; especially when they deal with underlying protection mechanisms fatty graft during cold storage. Fatty livers from male obese Zücker rats were...
The combined impact of an increasing demand for liver transplantation and a growing incidence nonalcoholic disease has provided the impetus development innovative strategies to preserve steatotic livers. A natural oxygen carrier, HEMO2life®, which contains M101 that is extracted from marine invertebrate, been used static cold storage (SCS) shown superior results in organ preservation. total 36 livers were procured obese Zucker rats randomly divided into three groups, i.e., control, SCS-24H +...
METHODSFatty liver grafts from male obese Zücker rats were conserved in UW and IGL-1 solutions for 24 h at 4 ℃ subjected to "ex vivo " normo-thermic perfusion (2 h; 37 ℃).Liver proteolysis tissue specimens perfusate was measured by reverse-phase high performance liquid chromatography.Total free amino acid release correlated with the activation of ubiquitin proteasome system (UPS: as chymotryptic-like activity 20S 19S proteasome), prevention injury (transaminases), mitochondrial (confocal...
Many factors may compromise the functional recovery of a harvested potential liver for engraftment. Normothermic machine perfusion (NMP) can revive hepatic metabolism ex vivo enabling subsequent transplantation. In this study, we evaluated 11 discarded livers' function utilizing NMP.Eleven consecutive livers underwent NMP 6 hours. Liver was defined by lactate levels ≤3 mmol/L and continuous bile production.Ten perfused were fatty. The median percentage macrosteatosis (MaS) microsteatosis...
<title>Abstract</title> Ionizable lipids are an essential component of lipid nanoparticles (LNPs) for efficient mRNA delivery. However, optimizing their chemical structures high protein expression, endosomal escape, and selective organ targeting remains challenging due to complex structure-activity relationships multistep synthesis. In this study, we introduce a rapid, high-throughput platform screening ionizable using two-step, scalable synthesis involving one-pot 3-component click-like...
Background Static cold storage (SCS) in preservation solutions is a critical step for steatotic liver grafts submitted to ischemia-reperfusion transplantation. Endothelial monolayer surface that covers graft endothelium named glycocalyx (GCX) protects against ischemia-injury but its alterations during UW, HTK and IGL-1 have been poorly investigated. In this study, we assessed the GCX constituents such as syndecan-1 (SDC-1), heparan sulfate (HS) glypican-1 (Gpc-1) after SCS solutions; well...