A5055502363- CRISPR and Genetic Engineering
- Neuroinflammation and Neurodegeneration Mechanisms
- Neurogenesis and neuroplasticity mechanisms
- Immune cells in cancer
- Pluripotent Stem Cells Research
- Asthma and respiratory diseases
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Heart Rate Variability and Autonomic Control
- Immune Response and Inflammation
- Cardiovascular and exercise physiology
- Advanced biosensing and bioanalysis techniques
- Fungal Infections and Studies
- Immune Cell Function and Interaction
- MicroRNA in disease regulation
- Respiratory and Cough-Related Research
- Hemodynamic Monitoring and Therapy
- SARS-CoV-2 and COVID-19 Research
- Virus-based gene therapy research
- Pancreatic function and diabetes
- Inflammasome and immune disorders
- Tuberculosis Research and Epidemiology
- Child Nutrition and Water Access
- Allergic Rhinitis and Sensitization
- Multiple Sclerosis Research Studies
- Ear and Head Tumors
Broad Institute
2020-2024
Massachusetts Institute of Technology
2020-2024
McGovern Institute for Brain Research
2020-2024
Sheba Medical Center
2009-2023
Tel Aviv University
2013-2023
University of Cambridge
2019-2023
Wellcome/MRC Cambridge Stem Cell Institute
2018-2023
Medical Research Council
2018-2021
Howard Hughes Medical Institute
2020-2021
Stem Cell Institute
2018
Bacteria and archaea are frequently attacked by viruses other mobile genetic elements rely on dedicated antiviral defense systems, such as restriction endonucleases CRISPR, to survive. The enormous diversity of suggests that more types systems exist than currently known. By systematic gene prediction heterologous reconstitution, here we discover 29 widespread cassettes, collectively present in 32% all sequenced bacterial archaeal genomes, mediate protection against specific bacteriophages....
Eukaryotic genomes contain domesticated genes from integrating viruses and mobile genetic elements. Among these are homologs of the capsid protein (known as Gag) long terminal repeat (LTR) retrotransposons retroviruses. We identified several mammalian Gag that form virus-like particles one LTR retrotransposon homolog, PEG10, preferentially binds facilitates vesicular secretion its own messenger RNA (mRNA). showed mRNA cargo PEG10 can be reprogrammed by flanking interest with Peg10's...
Abstract The recent outbreak of the novel coronavirus SARS-CoV-2, which causes COVID-19, can be diagnosed using RT-qPCR, but inadequate access to reagents and equipment has slowed disease detection impeded efforts mitigate viral spread. Alternative approaches based on combinations isothermal amplification CRISPR-mediated detection, such as SHERLOCK ( S pecific H igh ensitivity E nzymatic R eporter Un LOCK ing) technique, offer reduced dependence RT-qPCR equipment, previously reported methods...
A number of endogenous genes in the human genome encode retroviral gag -like proteins, which were domesticated from ancient retroelements. The paraneoplastic Ma antigen (PNMA) family members a capsid domain, but their ability to assemble as capsids and traffic between cells remains mostly uncharacterized. Here, we systematically investigate PNMA proteins find that PNMAs are secreted by cells. We determine PNMA2 forms icosahedral efficiently does not naturally encapsidate nucleic acids....
Tissue macrophages, including microglia, are notoriously resistant to genetic manipulation. Here, we report the creation of Adeno-associated viruses (AAV) variants that efficiently and widely transduce microglia tissue macrophages in vivo following intravenous delivery, with transgene expression up 80%. We use this technology demonstrate manipulation gene microglial ablation, thereby providing invaluable research tools for study these important cells.
Abstract It is now well‐established that the macrophage and microglial response to CNS demyelination influences remyelination by removing myelin debris secreting a variety of signaling molecules influence behaviour oligodendrocyte progenitor cells (OPCs). Previous studies have shown changes in microglia contribute age‐related decline efficiency remyelination. In this study, we show increase their expression proteoglycan NG2 with age, associated an altered micro‐niche generated aged, but not...
Objective. At present, there is no consensus on the best strategy for interpreting cardiopulmonary exercise test’s (CPET) results. This study aimed at assessing potential of using computer-aided algorithms to evaluate CPET data identifying chronic heart failure (CHF) and obstructive pulmonary disease (COPD). Methods. Data from 234 files Pulmonary Institute, Sheba Medical Center, Givat-Washington College, both in Israel, were selected this study. The included patients with confirmed primary...
Abstract Microglia, the tissue resident macrophages of CNS, are implicated in a broad range neurological pathologies, from acute brain injury to dementia. Here, we profiled gene expression variation primary human microglia isolated 141 patients undergoing neurosurgery. Using single cell and bulk RNA sequencing, defined distinct cellular populations acutely vivo -activated microglia, characterised dramatic switch microglial population composition suffering injury. We mapped quantitative trait...
Histoplasmosis is a common endemic human mycoses acquired mostly in the Americas. We reviewed 23 cases of histoplasmosis Israeli travelers; 22 had traveled to Central or South America and one North America. Fourteen been exposed bat habitats were symptomatic, presenting ≤ 3 months after their return. Asymptomatic patients ( N = 9) diagnosed during evaluation incidental radiological findings because travel partner suspected Histoplasma infection, 16–120 Serological testing was positive 75%...
Microglia, the resident immune cells of brain, rapidly change states in response to their environment, but we lack molecular and functional signatures different microglial populations. In this study, analyzed RNA expression patterns more than 76,000 individual microglia during development, old age after brain injury. Analysis uncovered at least nine transcriptionally distinct states, which expressed unique sets genes were localized using specific markers. The greatest heterogeneity was found...
Abstract We report 8 cases of persistent severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pneumonia in patients previously treated with anti-CD20 monoclonal antibodies. Polymerase chain reaction nasopharyngeal swabs for SARS-CoV-2 was negative most cases; viral cell cultures confirmed that viable SARS-Co-2 virus present. Four were anti-SARS-CoV-2 hyperimmune globulins rapid resolution disease.
Abstract Background Methacholine challenge tests (MCTs) are used to diagnose airway hyperresponsiveness (AHR) in patients with suspected asthma where previous diagnostic testing has been inconclusive. The test is time consuming and usually requires referral specialized centers. Simple methods predict AHR could help determine which should be referred MCTs, thus avoiding unnecessary testing. Here we investigated the potential use of baseline spirometry variables as surrogate markers for adults...
Cardiovascular reserve index (CVRI) was previously proposed as an estimate of the assumed (momentary) cardiovascular a function stroke volume (SV), systemic vascular resistance (SVR), respiratory rate (RR) and body surface area (BSA). Conversion through conventional hemodynamic equations reveals equivalent, simpler, vital signs based function. We evaluated association between CVRI diverse conditions along spectrum. retrospectively computed for each subject 3 existing patient databases. 1)...