A5036162103- Plant Pathogens and Fungal Diseases
- Mycotoxins in Agriculture and Food
- Plant Disease Resistance and Genetics
- Plant-Microbe Interactions and Immunity
- Yeasts and Rust Fungi Studies
- Fungal Biology and Applications
- CRISPR and Genetic Engineering
- Phytoplasmas and Hemiptera pathogens
- Genetically Modified Organisms Research
- Bioenergy crop production and management
- Wheat and Barley Genetics and Pathology
- Plant Pathogens and Resistance
- Salmonella and Campylobacter epidemiology
- Cocoa and Sweet Potato Agronomy
- Antibiotic Resistance in Bacteria
- Biotechnology and Related Fields
- Animal Genetics and Reproduction
- Genomics and Phylogenetic Studies
- Biofuel production and bioconversion
- Light effects on plants
- Escherichia coli research studies
- Transgenic Plants and Applications
- Chromosomal and Genetic Variations
- Plant tissue culture and regeneration
- Botanical Research and Chemistry
Agence Nationale de Sécurité Sanitaire de l’Alimentation, de l’Environnement et du Travail
2013-2024
Paris-Est Sup
2024
Stellenbosch University
2011-2022
Laboratoire de Recherche en Sciences Végétales
2018
Mycologie et Sécurité des Aliments
2006-2017
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2013
Ministry of Agriculture, Nature and Food Quality
2012
Fusarium species, particularly graminearum and F. culmorum, are the main cause of trichothecene type B contamination in cereals. Data on distribution genotypes cereals Europe scattered time space. Furthermore, a common core set related variables (sampling method, host cultivar, previous crop, etc.) that would allow more effective analysis factors influencing spatial temporal population distribution, is lacking. Consequently, based available data, it difficult to identify chemotype spread at...
The distribution and co‐occurrence of four Fusarium species their mycotoxins were investigated in maize samples from two susceptible cultivars collected at 14 localities South Africa during 2008 2009. Real‐time PCR was used to quantify the respective grain, quantified by multi‐toxin analysis using HPLC‐MS. In 2008, F. graminearum predominant associated with ear rot eastern Free State, Mpumalanga KwaZulu‐Natal provinces, while verticillioides Northwest, western State Northern Cape provinces....
The impact of five phenolic acids (ferulic, coumaric, caffeic, syringic, and p-hydroxybenzoic acids) on fungal growth type B trichothecene production by four strains Fusarium graminearum was investigated. All inhibited but the degree inhibition varied between strains. Our results suggested that more lipophilic are, higher is effect they have growth. Toxin accumulation in acid-supplemented liquid glucose, yeast extract, peptone cultures enhanced presence ferulic coumaric reduced acid. This...
Abstract Reducing production of type B trichothecenes by Fusarium graminearum on cereals is necessary to control contamination, prevent yield reduction and protect human animal health. Thus, an understanding how trichothecene biosynthesis induced essential. The effect ambient pH fungal growth, toxin expression TRI genes was studied during in vitro liquid culture F. minimal medium. Fungal development stopped at day 3 after a sharp drop the At same time, induction gene observed began...
Fusarium head blight (FHB) is a major cereal disease caused by complex of species. These species vary in importance depending on climatic conditions, agronomic factors or host genotype. In addition, can release toxic secondary metabolites. mycotoxins constitute significant food safety concern as they have health implications both humans and animals. The involved FHB differ their pathogenicity, ability to produce mycotoxins, fungicide sensitivity. Accurate exhaustive identification planta...
Foliar pathogens face heterogeneous environments depending on the maturity of leaves they interact with. In particular, nutrient availability as well defense levels may vary significantly, with opposing effects success infection. The present study tested which these factors have a dominant effect pathogen's development. Poplar leaf disks eight were inoculated poplar rust fungus Melampsora larici-populina using an innovative single-spore inoculation procedure. A set quantitative fungal traits...
In the context of pathogen surveillance, it is crucial to ensure interoperability and harmonized data. Several surveillance systems are designed compare bacteria identify outbreak clusters based on core genome MultiLocus Sequence Typing (cgMLST). Among different approaches available generate bacterial cgMLST, our research used an assembly-based approach (chewBBACA tool).
Leaf blotch caused by Alternaria spp. is a common disease in apple-producing regions. The usually associated with one phylogenetic species and complex, alternata the arborescens complex (A. SC), respectively. Both taxa may include apple pathotype, quarantine or regulated pathogen several countries. pathotype characterized production of host-selective toxin (HST) which involved pathogenicity towards apple. A cluster genes located on conditionally dispensable chromosomes (CDCs) this HST...
Pitch canker, caused by Fusarium circinatum, was first reported in a forestry nursery the Mpumalanga Province of South Africa 1990, and it has since spread to almost all nurseries country, where causes significant economic losses. The aim current study (i) identify sources F. circinatum contamination Karatara Western Cape (ii) manage disease implementing an oxidation reduction potential (ORP)-based sanitation method using hydrogen peroxide. irrigation water, planting tray inserts seeds were...
The rust fungi Melampsora larici‐populina (Mlp) and allii‐populina (Map) are the main phytosanitary constraints for commercial poplar cultivation in Europe. Although Mlp is more aggressive prevalent than Map, two species may co‐infect same tree or even leaf, making epidemiological surveys of each difficult to achieve. In this study, a new duplex real‐time PCR assay targeting was developed, based on single‐copy genes. This test proved be specific, inclusive successfully used detect quantify...
Abstract The presence of genetically modified organisms (GMO) is commonly assessed using real-time PCR methods targeting the most common transgenic elements found in GMOs. Once GM material has been established these screening methods, GMOs are further identified a battery each being specific one event and usually junction plant genome DNA insert. If, no GMO could be identified, an unauthorized suspected. In this context, aim work was to develop fast simple method obtain sequence transgene...
Fusarium head blight (FHB) of wheat occurs commonly in irrigation regions South Africa and less frequently dryland regions. Previous surveys species causing FHB identified isolates using morphological characters only. This study reports on a comprehensive characterisation pathogens conducted 2008 2009. Symptomatic heads were collected from the Northern Cape, KwaZulu-Natal (KZN), Bushveld eastern Free State (irrigation regions), one field Western Cape (dryland region). with species-specific...
Strengthening Plant Health research is a major challenge for Mediterranean countries. The diversity and fragmentation of the landscape in this region have weakened impacts national efforts. countries can benefit from coordination activities to increase efficiency impacts. initiative ‘Plant health priorities region’, led by International Centre Advanced Agronomic Studies (CIHEAM Bari)1 Euphresco network phytosanitary funding2 promote convergence programmes optimize use scarce funding...
The EU directive 2001/18/EC requires any genetically modified (GM) event to be stable. In the present work, a targeted Next-Generation Sequencing (NGS) approach using barcodes specifically tag each individual DNA molecules during library preparation was implemented detect mutations taking into account background noise due amplification and sequencing errors. method first showed efficient in detecting synthetic samples prepared with custom-synthesized mutated or non-mutated P35S sequences...