A5064744956- Cutaneous Melanoma Detection and Management
- Melanoma and MAPK Pathways
- Cancer Genomics and Diagnostics
- Immunotherapy and Immune Responses
- Autoimmune Bullous Skin Diseases
- Cancer Immunotherapy and Biomarkers
- Urticaria and Related Conditions
- Cancer Cells and Metastasis
- Sarcoma Diagnosis and Treatment
- Cutaneous lymphoproliferative disorders research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Dermatology and Skin Diseases
- CAR-T cell therapy research
- Nanoplatforms for cancer theranostics
- Mast cells and histamine
- 3D Printing in Biomedical Research
- Nail Diseases and Treatments
- NF-κB Signaling Pathways
- Immune Cell Function and Interaction
- Skin and Cellular Biology Research
- Chemotherapy-related skin toxicity
- Redox biology and oxidative stress
- Nitric Oxide and Endothelin Effects
- HER2/EGFR in Cancer Research
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
Shinshu University
2012-2022
Shinshu University Hospital
2008-2022
Ōtani University
2006-2019
Generation of reactive oxygen species (ROS) has been implicated in carcinogenic development melanoma, but the underlying molecular mechanism not fully elucidated. We studied expression and function superoxide-generating NADPH oxidase (Nox)4 human melanoma cells. Nox4 was up-regulated 13 20 cell lines tested. Silencing MM-BP cells by small interfering RNAs decreased ROS production thereby inhibited anchorage-independent growth tumorigenecity nude mice. Consistently, a general Nox inhibitor,...
Recent studies showed KIT gene aberrations in a substantial number of melanomas on acral skin and mucosa, suggesting the therapeutic benefit tyrosine kinase inhibitors, such as imatinib. We therefore examined expression mutations 4 primary 24 metastatic mucosal melanomas. Immunohistochemistry revealed moderate or strong protein 13 (48%) tumors. Sequence analysis K642E D820Y two metastases. Amplification was identified by real-time PCR tumors, including one that had K642E. Western blot...
Drug-induced hypersensitivity syndrome (DIHS) is a severe reaction to drugs which characteristically occurs after long latency period. In addition, human herpes virus 6 (HHV-6) reactivation characteristic finding in DIHS, has been known be related disease severity. Because DIHS generally treated by systemic corticosteroids, the natural clinical course not clear.Data for patients with both and HHV-6 were retrospectively collected from four hospitals.Data on 12 ranging age 21 76 years (median,...
Anti-programmed cell death-1 (anti-PD-1) antibody shows high therapeutic efficacy in patients with advanced melanoma. However, assessment of its activity can be challenging because tumour enlargement associated intratumoural inflammation. Because circulating DNA (ctDNA) correlates burden, we assessed the value ctDNA levels as an indicator changes. Quantification (BRAFmutant or NRASmutant) by droplet digital PCR 5 BRAF NRAS mutant melanoma during treatment course showed dynamic changes...
Background Anaplastic thyroid cancer is considered to be one of the most aggressive human malignancies, and mean survival time after diagnosis approximately six months, regardless treatments. This study aimed examine how EpCAM its related molecules are involved in characteristics anaplastic carcinoma. Methodology/Principal Findings Two differentiated cell lines (TPC-1 FTC-133), two (FRO, ACT-1) were analyzed for expression CD44 standard isoform (CD44s), variant isoforms, EpCAM, aldehyde...
Immune cell Toll-like receptors (TLRs) recognize conserved microbial components, leading to immune and inflammatory responses. However, TLRs are also expressed in cancer cells, including melanoma which express TLR2-4. TLR4 ligands have received attention as immunotherapies; therefore, we assessed the expression of human specimens (29 primary lesions 28 metastatic lesions) representing different types melanoma. A high percentage (≥ 90%) TLR4, judged by immunohistochemistry. Next, role...
Abstract Background While molecularly targeted therapies and immune checkpoint inhibitors have improved the prognosis of advanced melanoma, biomarkers are required to monitor drug responses. Circulating tumor cells (CTCs) released from primary and/or metastatic tumors into peripheral blood. We examined whether CTCs potential as by checking number CTCs, well BRAF genotype individual in melanoma patients undergoing BRAF/MEK inhibitor treatment. Methods were isolated blood using a high-density...
We established a novel melanoma cell line, SMYM‐PRGP, which was non‐tumorigenic in vivo , from an acral radial growth phase under low‐oxygen environment. SMYM‐PRGP wild‐type for known mutation sites the BRAF and NRAS genes, showed focal amplification of human telomerase reverse transcriptase cyclin D1 genes as well fibroblast factor‐3 factor‐4 genes. Neither nor copy number loss CDKN2A gene observed. The p16 INK4A protein expressed at level equal to that normal melanocytes. Among various...