A5004693750- CAR-T cell therapy research
- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- Systemic Sclerosis and Related Diseases
- Cutaneous Melanoma Detection and Management
- Melanoma and MAPK Pathways
- Immune Cell Function and Interaction
- Dermatologic Treatments and Research
- Vascular Tumors and Angiosarcomas
- Nail Diseases and Treatments
- Cancer and Skin Lesions
- Cutaneous lymphoproliferative disorders research
- Nonmelanoma Skin Cancer Studies
- Dermatological and Skeletal Disorders
- Fungal Infections and Studies
- MicroRNA in disease regulation
- Autoimmune Bullous Skin Diseases
- Immune cells in cancer
- Plant Pathogens and Fungal Diseases
- Skin and Cellular Biology Research
- Dermatology and Skin Diseases
- Inflammatory Myopathies and Dermatomyositis
- Psoriasis: Treatment and Pathogenesis
- Cancer-related molecular mechanisms research
- Lipid metabolism and disorders
Kumamoto University
2016-2025
Omuta hospital
1997-2024
Prefectural University of Kumamoto
2015-2023
Yamagata City Hospital Saiseikan
2015-2023
The University of Tokyo
2010-2023
Sanyo-Onoda City University
2020-2023
Okayama University
1959-2022
Tsuyama Chuo Hospital
2022
Okayama Prefecture
2022
Kumamoto University Hospital
2019-2020
To report the clinical features of myasthenia gravis (MG) induced by treatment with immune checkpoint inhibitors using 2-year safety databases based on postmarketing surveys in Japan.We studied 10,277 patients cancer who had received monotherapy either nivolumab or ipilimumab between September 2014 and August 2016. As control group, 105 idiopathic MG were used.There 12 cases (0.12%) among 9,869 been treated nivolumab, but none 408 ipilimumab. These included 6 men women a mean age 73.5 ± 6.3...
Recently emerging cancer immunotherapies combine the applications of therapeutics to disrupt immunosuppressive conditions in tumor-bearing hosts. In this study, we found that targeting proinflammatory cytokine IL6 enhances tumor-specific Th1 responses and subsequent antitumor effects mice. blockade upregulated expression immune checkpoint molecule programmed death-ligand 1 (PD-L1) on melanoma cells. This PD-L1 induction was canceled IFNγ-deficient mice or CD4+ T cell-depleted mice,...
An 80‐year‐old man, who developed multiple lymph node and skin metastasis of malignant melanoma, received nivolumab monotherapy. Two weeks after the first dose, he experienced anorexia fatigue, suffered from progressive, severe dyspnea muscle weakness. We diagnosed him with myocarditis, myositis, myasthenic crisis induced by nivolumab. commenced steroid therapy, immune absorption plasma exchange i.v. immunoglobulin succeeded in saving his life. Because serum level anti‐acetylcholine receptor...
Recent accumulating evidence indicates a crucial involvement of macrophage lineage in the pathogenesis systemic sclerosis (SSc). To analyze assembly monocyte/macrophage population, we evaluated expression CD163 and CD204 various activated markers, inflammatory cells skin peripheral blood mononuclear (PBMCs) derived from patients with SSc.Skin biopsy specimens 6 healthy controls 10 SSc (7 limited cutaneous 3 diffuse SSc) were analyzed by immunohistochemistry using monoclonal antibody against...
Among IL-17 families, IL-17A and IL-17F share amino acid sequence similarity bind to IL-17R type A. signaling is implicated in the pathogenesis of various autoimmune diseases, but its role regulatory mechanism extracellular matrix expression contribution phenotype systemic sclerosis (SSc) both remain be elucidated. This study revealed that was significantly increased involved skin sera SSc patients, whereas levels did not increase. In contrast, A fibroblasts decreased comparison with normal...
Abstract Systemic and localized scleroderma (SSc LSc) is characterized by excessive deposition of collagen tissue fibrosis in the skin. Although they have fundamental common characteristics including autoimmunity, little known about exact mechanism that mediates expression these disorders. In current study, we tried to evaluate possibility microRNAs (miRNAs) play some roles pathogenesis seen diseases. miRNA patterns were evaluated array analysis, real-time PCR, situ hybridization. The...
Abstract Previous reports indicated the significance of TGF-β signaling in pathogenesis systemic sclerosis. We tried to evaluate possibility that microRNAs (miRNAs) play a part type I collagen upregulation seen normal fibroblasts stimulated with exogenous and sclerosis (SSc) fibroblasts. miRNA expression profile was evaluated by PCR array real-time PCR. The protein determined immunoblotting. In vivo detection paraffin section performed situ hybridization. Several miRNAs were found be...
Immune checkpoint inhibitors blocking the interaction between programmed death-1 (PD-1) and PD-1 ligand-1 (PD-L1) are revolutionizing cancer immunotherapies with durable clinical responses. Although high expression of PD-L1 in tumor tissues has been implicated to correlate better response anti-PD-1 therapies, this association controversial. In study, characterize immune microenvironment tumors, we examined mRNA levels immune-related genes characterized T cell repertoire tumors 13 melanoma...
Psoriasis is a chronic inflammatory skin disease characterized by intense proliferation and abnormal differentiation of keratinocytes, although the pathogenesis still not completely clarified.We investigated mechanism keratinocyte seen in psoriasis, focusing on microRNA (miRNA).miRNAs were extracted from tissues sera atopic dermatitis healthy control. To determine pathogenic miRNAs, we performed miRNA polymerase chain reaction (PCR) array analysis. The results confirmed with quantitative...
Abstract Background The incidence of melanoma among those an Asian ethnicity is lower than in Caucasians; few large‐scale studies that include follow‐up data have been reported. Objectives To investigate the clinical characteristics Japanese patients with and to evaluate prognostic factors. Methods Detailed patient information was collected from database Melanoma Study Group Skin Cancer Society. American Joint Committee on seventh Edition system used for TNM classification. Kaplan‐Meier...
mRNA-based vaccines have been used globally to eradicate the coronavirus-disease 2019 (COVID-19) pandemic. Vaccine efficacy and adverse reactions depend on immune responses, such as proinflammatory cytokine production lymphocyte activation. We conducted a prospective cohort study investigate relationships among specific antibody titers, reactions, production, immune-regulatory microRNA (miRNA) levels in serum extracellular vesicles (EVs) after COVID-19 vaccination (BNT162b2). Local second...
Abstract Acral melanoma is an aggressive type of with unknown origins. It the most common in individuals dark skin and notoriously challenging to treat. We examine exome sequencing data 139 tissue samples, spanning different progression stages, from 37 patients. find that 78.4% melanomas display clustered copy number transitions focal amplifications, recurring predominantly on chromosomes 5, 11, 12, 22. These complex genomic aberrations are typically shared across all stages individual TERT...
Aberrant immune responses to viral pathogens contribute pathogenesis, but our understanding of pathological caused by viruses within the human virome, especially at a population scale, remains limited. We analyzed whole-genome sequencing datasets 6,321 Japanese individuals, including patients with autoimmune diseases (psoriasis vulgaris, rheumatoid arthritis (RA), systemic lupus erythematosus (SLE), pulmonary alveolar proteinosis (PAP) or multiple sclerosis) and coronavirus disease 2019...
Background Senile hemangioma, so-called cherry angioma, is known as the most common vascular anomalies specifically seen in aged skin. The pathogenesis of its abnormal angiogenesis still unclear. Methodology/Principal Findings In this study, we found that senile hemangioma consisted clusters proliferated small channels upper dermis, indicating tumor categorized a tumor. We then investigated mechanism endothelial proliferation focusing on microRNA (miRNA). miRNA PCR array analysis revealed...
Abstract Methods have been established to generate dendritic cells (DCs) from mouse and human embryonic stem (ES) cells. We designated them as ES-DCs models demonstrated the induction of anti-cancer immunity prevention autoimmune disease by in vivo administration genetically engineered ES-DCs. For future clinical application ES-DCs, histoincompatibility between patients be treated available ES ethical concerns associated with may serious obstacles. However, recently developed induced...
Background Basic fibroblast growth factor (bFGF, FGF‐2) has been described as a multipotent cytokine that regulates cell well differentiation, matrix composition, chemotaxis, adhesion and migration in numerous types. It is known bFGF stimulates proliferation of cultured fibroblasts. However, the detailed mechanism induced by vitro still remains to be elucidated. Objectives We investigated precise effects on signalling pathways responsible for bFGF‐induced human dermal fibroblasts (HDFs)....
Objectives. microRNAs (miRNAs) play a part in various cellular activities. However, the role of miRNA SSc is not fully understood. This study investigated expression and miR-92a patients evaluated possibility that involved pathogenesis this disease. Methods. Serum samples were obtained from 61 patients. mRNAs purified serum levels miR-135 measured with quantitative real-time PCR. dermal fibroblasts was also determined by Immunoblotting performed to detect MMP-1 protein. Results. The median...
Recently, increased evidence has shown that serum micro (mi)RNA levels are a useful biomarker for the diagnosis, prognosis and therapeutic value of various diseases. However, miRNA not been investigated in patients with systemic sclerosis (SSc), to our knowledge.To investigate possibility Homo sapiens miR-142 stem-loop (hsa-miR-142-3p), one miRNAs regulating expression integrin αV, could be specific disease marker SSc.Serum samples were obtained from 61 SSc 20 healthy controls. Patients...