A5045391986- Systemic Sclerosis and Related Diseases
- Autoimmune Bullous Skin Diseases
- Inflammatory Myopathies and Dermatomyositis
- Dermatologic Treatments and Research
- Mast cells and histamine
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Eosinophilic Disorders and Syndromes
- Urticaria and Related Conditions
- Cell Adhesion Molecules Research
- Dermatology and Skin Diseases
- Synthesis and Biological Evaluation
- T-cell and B-cell Immunology
- Skin Diseases and Diabetes
- Systemic Lupus Erythematosus Research
- Wound Healing and Treatments
- Skin and Cellular Biology Research
- Connective Tissue Growth Factor Research
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Dermatological and Skeletal Disorders
- Pain Mechanisms and Treatments
- Circadian rhythm and melatonin
- Genital Health and Disease
- Pressure Ulcer Prevention and Management
- Cancer and Skin Lesions
University of Fukui
2015-2024
Takenaka (Japan)
2023
Futaba (Japan)
2012-2023
Shionogi (Japan)
2012-2021
University of Fukui Hospital
2020
Kanazawa University
2006-2016
Isesaki Fukushima Hospital
2015
Isesaki Municipal Hospital
2015
Kanazawa Medical University
2004-2013
Japan Broadcasting Corporation (Japan)
2013
Objective To identify similarities and differences in the clinical features of adult Japanese patients with individual anti-aminoacyl-tRNA synthetase antibodies (anti-ARS Abs). Methods This was a retrospective analysis 166 anti-ARS Abs detected by immunoprecipitation assays. These had visited Kanazawa University Hospital or collaborating medical centers from 2003 to 2009. Results Anti-ARS Ab specificity included anti-Jo-1 (36%), anti-EJ (23%), anti-PL-7 (18%), anti-PL-12 (11%), anti-KS (8%),...
<h3>Objective</h3> To clarify the association of clinical and prognostic features with dermatomyositis (DM)-specific autoantibodies (Abs) in adult Japanese patients DM. <h3>Design</h3> Retrospective study. <h3>Setting</h3> Kanazawa University Graduate School Medical Science Department Dermatology collaborating medical centers. <h3>Patients</h3> A total 376 consecutive DM who visited our hospital or centers between 2003 2008. <h3>Main Outcome Measures</h3> Clinical laboratory characteristics...
To identify the 140-kd autoantigen recognized by anti-155/140 autoantibodies that are associated with adult cancer-associated dermatomyositis (DM) and juvenile DM to determine clinical relevance of antibodies in a large cohort.Sera from 456 patients were assessed for presence immunoprecipitation using K562 cell extracts as substrate. Using Western blotting, we then examined whether anti-155/140-positive sera transcription intermediary factor 1α (TIF-1α), TIF-1β, TIF-1γ. The associations...
Objectives Myositis-specific autoantibodies (MSAs) are useful tools for identifying clinically homogeneous subsets and predicting prognosis of patients with idiopathic inflammatory myopathies (IIM) including polymyositis (PM) dermatomyositis (DM). Recent studies have shown that anti-NXP2 antibody (Ab) is a major MSA in juvenile (JDM). In this study the frequencies clinical associations Ab were evaluated adult IIM. Methods Clinical data serum samples collected from 507 Japanese IIM (445 DM 62...
Signaling thresholds influence the balance between humoral immunity and autoimmunity. Cell surface CD19 regulates intrinsic Ag receptor-induced B lymphocyte signaling thresholds, transgenic mice that overexpress by 3-fold generate spontaneous autoantibodies in a genetic background not associated with To quantify extent genetically determined differences expression of single cell molecule can autoantibody production, we have assessed autoimmunity C57BL/6-transgenic mouse line subtle 15-29%...
Myositis-specific autoantibodies (MSAs) are a useful tool in diagnosis, defining clinical subsets and predicting prognosis of dermatomyositis (DM) polymyositis (PM). In this study, we identified novel MSA reactive with 155 140 kDa nuclear proteins [anti-155/140 antibody (Ab)] determined the feature DM patients positive for autoantibody (autoAb).Sera from 52 Japanese DM, 9 PM, 48 systemic lupus erythematosus (SLE), 126 sclerosis 18 idiopathic interstitial pneumonia were examined by...
To determine phenotypic and functional abnormalities of blood B cell subsets in patients with systemic sclerosis (SSc).Cell surface marker expression was determined by flow cytometry. Spontaneous apoptosis evaluated annexin V cytometric analysis. IgG production isolated IgD- memory cells examined enzyme-linked immunosorbent assay.The numbers CD27- naive from SSc were increased compared normal control cells, while expressing medium levels CD27 plasmablasts high reduced. In contrast, the...
To determine serum levels of BAFF, a potent B cell survival factor, in patients with systemic sclerosis (SSc) and relate the results to clinical features SSc.Serum BAFF 83 SSc were examined by enzyme-linked immunosorbent assay (ELISA). In longitudinal study, 131 samples obtained from 21 analyzed. The expression messenger RNA (mRNA) skin was quantified real-time reverse transcription-polymerase chain reaction. receptor (BAFFR) on CD19+ cells assessed flow cytometry. production IgG...
CD20 plays a role in human B cell proliferation and is an effective target for immunotherapy. In this study, mouse expression biochemistry were assessed the first time using new panel of CD20‐specific mAb, with function CD20‐deficient (CD20–/–) mice. was restricted initiated during late pre‐B development. The frequency density increased maturation bone marrow, subpopulation transitional IgMhi cells expressing higher levels than majority mature recirculating cells. Transitional T1 spleen also...
The tight-skin (TSK/+) mouse, a genetic model for human systemic sclerosis (SSc), develops cutaneous fibrosis and autoantibodies against SSc-specific target autoantigens. Although molecular mechanisms explaining the development of autoimmunity in SSc patients or TSK/+ mice remain unknown, we recently demonstrated that overexpress CD19, an important regulatory molecule expressed by B lymphocytes. cells from CD19-deficient are hyporesponsive to transmembrane signals, while overexpressing CD19...
SUMMARY To determine the role of chemokines in pathogenesis systemic sclerosis (SSc), we examined serum levels, spontaneous production by peripheral blood mononuclear cells (PBMC), and histological distribution affected skin, MCP-1, MIP-1α MIP-1β SSc patients. Serum levels these were ELISA 58 patients with 20 normal controls. The culture supernatants from PBMC also measured ELISA. MIP-1α, significantly elevated compared Elevated MCP-1 correlated presence pulmonary fibrosis. expression skin...
The tight-skin (TSK/+) mouse, a genetic model for human systemic sclerosis (SSc), develops cutaneous fibrosis and autoantibodies against SSc-specific target autoantigens. Although molecular mechanisms explaining the development of autoimmunity in SSc patients or TSK/+ mice remain unknown, we recently demonstrated that overexpress CD19, an important regulatory molecule expressed by B lymphocytes. cells from CD19-deficient are hyporesponsive to transmembrane signals, while overexpressing CD19...
Among IL-17 families, IL-17A and IL-17F share amino acid sequence similarity bind to IL-17R type A. signaling is implicated in the pathogenesis of various autoimmune diseases, but its role regulatory mechanism extracellular matrix expression contribution phenotype systemic sclerosis (SSc) both remain be elucidated. This study revealed that was significantly increased involved skin sera SSc patients, whereas levels did not increase. In contrast, A fibroblasts decreased comparison with normal...
Antimelanoma differentiation‐associated protein (anti‐MDA)5 antibodies are associated with rapidly progressive interstitial lung disease (RP‐ILD) in patients clinically amyopathic dermatomyositis (CADM) or (DM). We aimed to evaluate the relevance of monitoring anti‐MDA5 antibody levels for management RP‐ILD CADM DM. Twelve (n = 10) DM 2) accompanied by were included. Baseline characteristics and outcomes recorded. Serial measurements measured. All treated corticosteroids, tacrolimus...
Paraneoplastic encephalitis with antibodies against NR1/NR2 heteromers of the NMDA receptor associates frequently ovarian teratoma and has recently been established as a distinct clinical entity.1 Most patients are young women who develop syndrome prodromal cold-like illness, intractable seizures, psychosis, dyskinesia, hypoventilation.1,2 However, about 40% do not have detectable tumor,3 treatment these remains unclear. We report patient anti-NR1/NR2 without showed nearly complete recovery...
Abstract Previous reports indicated the significance of TGF-β signaling in pathogenesis systemic sclerosis. We tried to evaluate possibility that microRNAs (miRNAs) play a part type I collagen upregulation seen normal fibroblasts stimulated with exogenous and sclerosis (SSc) fibroblasts. miRNA expression profile was evaluated by PCR array real-time PCR. The protein determined immunoblotting. In vivo detection paraffin section performed situ hybridization. Several miRNAs were found be...
Abstract Mice s.c. injected with bleomycin, an experimental model for human systemic sclerosis, develop skin and lung fibrosis, which is mediated by inflammatory cell infiltration. This process highly regulated multiple adhesion molecules does not require Ag sensitization. To assess the role of in this pathogenetic process, bleomycin-induced fibrosis was examined mice lacking molecules. L-selectin and/or ICAM-1 deficiency inhibited decreased Th2 Th17 cytokines increased Th1 cytokines. In...
Although transforming growth factor β (TGFβ) and connective tissue (CTGF) have been considered to play central roles in the pathogenesis of systemic sclerosis (SSc), other cytokines may also be crucial for development SSc. The aim this study was examine T helper skin fibrosis.To compare Th1, Th2, Th17 cytokines, we examined bleomycin-induced SSc mice deficient interferon-γ (IFNγ), interleukin-4 (IL-4), IL-17A. mechanism by which IL-17A contributes fibrosis investigated vivo vitro. outcome...