A5061952101- DNA and Nucleic Acid Chemistry
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- RNA Interference and Gene Delivery
- Optical Network Technologies
- Advanced Fiber Laser Technologies
- Photonic and Optical Devices
- Photonic Crystal and Fiber Optics
- Rheumatoid Arthritis Research and Therapies
- Bacteriophages and microbial interactions
- RNA modifications and cancer
- Glass properties and applications
- Advanced Fiber Optic Sensors
- RNA Research and Splicing
- Semiconductor Lasers and Optical Devices
- Chronic Lymphocytic Leukemia Research
- Chemical Synthesis and Analysis
- Lymphoma Diagnosis and Treatment
- Solid State Laser Technologies
- Advanced Photonic Communication Systems
- Molecular Junctions and Nanostructures
- Bacterial Genetics and Biotechnology
- Protein Structure and Dynamics
- Systemic Lupus Erythematosus Research
- Photorefractive and Nonlinear Optics
Konan University
2016-2025
Tokyo Women's Medical University
2013-2023
The University of Tokyo
2005-2022
Tokyo Women's Medical University Hospital
2015-2022
Jikei University School of Medicine
1990-2021
Innovative Research (United States)
2010-2020
National Institute of Health Sciences
2001-2019
Toin University of Yokohama
2019
Institute of Rheumatology
2015-2017
Katsushika Medical Center
2016
With the goal of being able to create optical devices for telecommunications industry, we investigated effects 810-nm, femtosecond laser radiation on various glasses. By focusing beam through a microscope objective, successfully wrote transparent, but visible, round-elliptical damage lines inside high-silica, borate, soda lime silicate, and fluorozirconate (ZBLAN) bulk Microellipsometer measurements damaged region in pure Ge-doped silica glasses showed 0.01–0.035 refractive-index increase,...
Thermodynamic parameters for prediction of RNA duplex stability are reported. One parameter initiation and 10 helix propagation derived from enthalpy free-energy changes formation by 45 oligonucleotide duplexes. The oligomer sequences were chosen to maximize reliability secondary structure predictions. Each the nearest-neighbor is well-represented among oligonucleotides, minimize experimental errors in delta GO at 37 degrees C. These predict melting temperatures most duplexes within 5 This...
Abstract To improve the previous DNA/DNA nearest-neighbor parameters, thermodynamic parameters (Δ H °, Δ S ° and G °) of 50 duplexes were measured. Enthalpy change a helix initiation factor is also considered though reported recently did not contain factor. A for duplex determined here was same as that RNA/RNA (ΔG° 37 = 3.4 kcal/mol). The improved reproduced only these experimental values used but 15 other obtained in different studies. Comparing with those RNA/DNA, generally most stable...
The effect of molecular crowding on the structure and stability biomolecules has become a subject increasing interest because it can clarify how behave under cell-mimicking conditions. Here, we quantitatively analyzed effects thermodynamics antiparallel G-quadruplex formation via Hoogsteen base pairs hairpin-looped duplex (HP duplex) Watson-Crick pairs. free energy change at 25 degrees C for decreased from -3.5 to -5.5 kcal mol(-1) when concentration poly(ethylene glycol) 200 was increased 0...
Objectives Myositis-specific autoantibodies (MSAs) are useful tools for identifying clinically homogeneous subsets and predicting prognosis of patients with idiopathic inflammatory myopathies (IIM) including polymyositis (PM) dermatomyositis (DM). Recent studies have shown that anti-NXP2 antibody (Ab) is a major MSA in juvenile (JDM). In this study the frequencies clinical associations Ab were evaluated adult IIM. Methods Clinical data serum samples collected from 507 Japanese IIM (445 DM 62...
Molecularly imprinted polymer gel with embedded gold nanoparticle was prepared on a substrate of chip for surface plasmon resonance (SPR) sensor fabricating an SPR sensitive to low molecular weight analyte. The sensing is based swelling the that triggered by analyte binding event within gel. causes greater distance between nanoparticles and substrate, shifting dip curve higher angle. synthesis conducted radical polymerization mixture acrylic acid, N-isopropylacrylamide,...
The thermodynamics of DNA duplex structures in the presence high concentrations cosolutes solution were investigated to discern nucleic acid and functions living cells. In ethylene glycol (EG) poly(ethylene glycol) (PEG) (MW = 200−8000), stability oligomer duplexes with differing nucleotide length varied, depending on as well size PEG. It was also revealed that decrease water activity is primary factor for destabilization short (8-mer) by addition molecular weight PEGs low other cosolutes....
We now present the first example in which triplet repeat DNAs adopt i-motif structure at neutral pH by molecular crowding. Crowding stabilized and pKa of N3 cytosine was raised such a microenvironment. Molecular crowding is known to accelerate formation multi-stranded while repeats single-strand structure.
Almost all biochemical reactions in vitro have been investigated through numerous experiments conducted dilute solutions containing low concentrations of solutes. However, biomacromolecules such as nucleic acids, proteins, and polysaccharides are designed to function and/or form their native structures a living cell high biomacromolecules, substrates, cofactors, salts, so on. In the present study, we demonstrated quantitatively effect molecular crowding on stabilities G-quadruplex d(G4T4G4)....
In the current study, we used a combination of gel electrophoresis, circular dichroism, and UV melting analysis to investigate structure stability G-quadruplexes formed by long telomeric DNAs from Oxytricha human, where length repeat (n) = 4 12. We found that DNAs, which have sequence (TTTTGGGG)n, folded into intramolecular intermolecular depending on ionic conditions, whereas human (TTAGGG)n, only in all tested conditions. further estimated thermodynamic parameters G-quadruplex. stabilities...
A molecularly imprinted polymer with immobilized Au nanoparticles (Au-MIP) is reported as a novel type of sensing material. The mechanism based upon the variable proximity in polymer, which exhibits selective binding given analyte accompanied by swelling that causes blue-shift plasmon absorption band nanoparticles. Using adrenaline model analyte, it was shown molecular imprinting effectively enhanced sensitivity and selectivity, accordingly, Au-MIP selectively detects at 5 μM. combination...
Using a porphyrin-functionalized graphene-modified electrode, an electrochemical impedance peptide sensor for label-free detection of cyclin A2 has been constructed. This can not only detect protein in cancer cells but also differentiate from normal ones. Furthermore, the assay provides potential using simple technique to estimate efficiency anticancer drugs therapy. Detailed facts importance specialist readers are published as "Supporting Information". Such documents peer-reviewed,...
We systematically and quantitatively investigated the structure thermodynamics of G-quadruplexes RNAs corresponding DNAs same sequences under molecular crowding conditions that mimic high osmotic stress induced by numerous molecules inside living cells. Structural analyses demonstrated various telomere RNA folded into parallel-stranded in a manner independent surrounding with different cations both dilute conditions. In contrast, DNA showed structural polymorphism. Moreover, we were more...
Significance Alterations in nonduplex structures could play roles during DNA replication the progression of cancer and other intractable diseases. These noncanonical differ topologically from each other. However, role these differences diseases remains unknown. In this study, we found that presence i-motif template caused polymerase to stall immediately before structures. The are more efficient than structures, such as G-quadruplexes hairpins, although their thermodynamic stabilities...
A G-quadruplex is a four-stranded DNA structure featuring stacked guanine tetrads, G-quartets. Formation of in telomere can inhibit telomerase activity; therefore, development G-quadruplex-ligands, which induce and/or stabilize G-quadruplexes, has become an area great interest. Phthalocyanine derivatives have substantial potential as high-affinity G-quadruplex-ligands because these planar chromophores are similar size and shape to the Here, we focus on latest findings phthalocyanine discuss...
Thioflavin T (ThT), a typical probe for protein fibrils, also binds human telomeric G-quadruplexes with fluorescent light-up signal change and high specificity against DNA duplexes. Cell penetration low cytotoxicity of fibril probes having been widely established, modifying ThT other is an attractive means generating new G-quadruplex ligands. Thus, elucidating the binding mechanism important design drugs based on ThT. Here, we investigated several variants sequence in presence monovalent...
Exosomes are emerging as ideal drug delivery vehicles due to their biological origin and ability transfer cargo between cells. However, rapid clearance of exogenous exosomes from the circulation well aggregation shedding surface proteins during storage limit clinical translation. Here, we demonstrate highly controlled reversible functionalization exosome surfaces with well-defined polymers that modulate exosome’s physiochemical pharmacokinetic properties. Using cholesterol-modified DNA...