A5067683642- Cancer Immunotherapy and Biomarkers
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Colorectal Cancer Surgical Treatments
- Hematopoietic Stem Cell Transplantation
- FOXO transcription factor regulation
- Salivary Gland Tumors Diagnosis and Treatment
- Head and Neck Cancer Studies
- Cancer Genomics and Diagnostics
- Genomics and Chromatin Dynamics
- Immune Cell Function and Interaction
- Colorectal Cancer Treatments and Studies
- Neuroblastoma Research and Treatments
- Reproductive System and Pregnancy
- Healthcare cost, quality, practices
- Hepatitis C virus research
- RNA Research and Splicing
- Acute Lymphoblastic Leukemia research
- Mechanisms of cancer metastasis
- Molecular Biology Techniques and Applications
- Cancer Mechanisms and Therapy
- Single-cell and spatial transcriptomics
- vaccines and immunoinformatics approaches
Icahn School of Medicine at Mount Sinai
2021-2024
University of Chicago
2016-2023
Northwestern University
2018-2019
Immune checkpoint inhibitors blocking the interaction between programmed death-1 (PD-1) and PD-1 ligand-1 (PD-L1) are revolutionizing cancer immunotherapies with durable clinical responses. Although high expression of PD-L1 in tumor tissues has been implicated to correlate better response anti-PD-1 therapies, this association controversial. In study, characterize immune microenvironment tumors, we examined mRNA levels immune-related genes characterized T cell repertoire tumors 13 melanoma...
To investigate the link between genomic landscape of cancer cells and immune microenvironment in tumor tissues, we characterized somatic mutations tumor-infiltrating lymphocytes (TILs) malignant pleural mesothelioma (MPM), including mutation/neoantigen load, spatial heterogeneity TILs (T-cell receptor β (TCRβ) repertoire), expression profiles immune-related genes using specimens three different sites (anterior, posterior, diaphragm) obtained from six MPM patients. Integrated analysis...
Abstract Mutations in isocitrate dehydrogenases 1 and 2 (IDH mut ) are present a variety of cancers, including glioma, acute myeloid leukemia (AML), melanoma, cholangiocarcinoma. These mutations promote hypermethylation, yet it is only favorable prognostic marker for reasons that unclear. We hypothesized the patterns DNA methylation, transcriptome profiles, would vary among IDH especially gliomas. Using Illumina 450K RNA-Seq data from The Cancer Genome Atlas, we show 365,092 analyzed CpG...
// Taigo Kato 1, * , Tatsuo Matsuda Yuji Ikeda Jae-Hyun Park 1 Matthias Leisegang 2, 3 Sachiko Yoshimura 4 Tetsuro Hikichi Makiko Harada Makda Zewde Sho Sato 5 Kosei Hasegawa Kazuma Kiyotani and Yusuke Nakamura 6 Department of Medicine, The University Chicago, IL 60637, USA 2 Institute Immunology – Campus Buch, Charité Universitätsmedizin Berlin, Berlin 13125, Germany Health, 10117, OncoTherapy Science Inc., Kawasaki, Kanagawa 213-0012, Japan Gynecologic Oncology, Saitama...
While multiple post-translational modifications have been reported to regulate the function of epidermal growth factor receptor (EGFR), effect protein methylation on its has not well characterized. In this study, we show that WHSC1L1 mono-methylates lysine 721 in tyrosine kinase domain EGFR, and leads enhanced activation downstream ERK cascade without EGF stimulation. We also EGFR K721 mono-methylation only affects cytoplasmic but nuclear EGFR. WHSC1L1-mediated nucleus interaction with PCNA...
Wolf-Hisrchhorn Syndrome Candidate 1-Like 1 (WHSC1L1) is a protein lysine methyltransferase that recurrently amplified (8p11.23) in patients with squamous cell carcinoma of the head and neck (SCCHN). In this study, we investigated oncogenic role WHSC1L1 SCCHN. Using immunohistochemistry on tissue microarrays locoregionally advanced SCCHN, found significantly overexpressed associated poor grade heavy smoking history. Knockdown expression resulted significant growth suppression reduction H3K36...
T‐lymphokine‐activated killer cell‐originated protein kinase ( TOPK ) plays critical roles in cancer cell proliferation as well maintenance of stem cells CSC ). Small lung SCLC has highly aggressive phenotype , reveals early spread to distant sites, and results dismal prognosis with little effective treatment. In this study, we demonstrate that expression was upregulated both lines primary tumors. Similar si RNA ‐mediated knockdown effects, treatment a potent inhibitor, OTS 514, effectively...
Tumor draining lymph nodes (TDLNs) are located in the routes of lymphatic drainage from a primary tumor and have highest risk metastasis various types solid tumors. TDLNs also considered as tissue to activate antitumor immunity, where antigen-specific effector T cells generated. However, cell receptor (TCR) repertoires not been well characterized. We collected 23 colorectal cancer tumors with 203 with/without metastatic (67 were metastasis-positive remaining 136 metastasis-negative)...
Purpose: Squamous cell carcinoma of the head and neck (SCCHN) is a lethal cancer with suboptimal 5-year overall survival approximately 50% surgery and/or definitive chemoradiotherapy. Novel treatments are thus urgently awaited. Immunotherapy checkpoint blockade has emerged as promising option for patients recurrent/metastatic SCCHN; however, it not been investigated in curative-intent setting yet. The purpose this study was to investigate T-cell receptor repertoire tumor microenvironment...
Acute graft-versus-host disease (GVHD) is a major cause of mortality in patients undergoing hematopoietic cell transplantation (HCT) for hematologic malignancies. The skin the most commonly involved organ GVHD. Elafin, protease inhibitor overexpressed inflamed epidermis, was previously identified as diagnostic biomarker GVHD; however, this finding restricted to subset with isolated main driver nonrelapse (NRM) HCT recipients gastrointestinal (GI) Two biomarkers, Regenerating islet-derived 3a...
Squamous cell carcinoma of the head and neck (SCCHN) is a malignancy with poor outcomes, thus novel therapies are urgently needed. We recently showed that WHSC1 necessary for viability SCCHN cells through H3K36 di-methylation. Here, we report identification its substrate, histone H1, WHSC1-mediated H1.4K85 mono-methylation may enhance stemness features in cells. To identify proteins interacting cells, immunoprecipitation mass spectrometry identified H1 as WHSC1-interacting candidate. In...
OBJECTIVE Maternal embryonic leucine zipper kinase (MELK) is receiving an attention as a therapeutic target in various types of cancers. In this study, we aimed to evaluate the prognostic significance MELK expression ovarian cancer using clinical samples, and assessed efficacy small molecule inhibitor, OTS167, patient-derived cells well cell lines. METHODS Expression levels 11 lines were confirmed by western blotting. Inhibitory concentration OTS167 was determined colorimetric assay....
<p>Supplementary Figure 1. TCR-β DI in HPV-positive patients (excluding smokers with {greater than or equal to}10packyear history) versus HPV-negative <10pack-year (Mann-Whitney U-test, p=0.004). Supplementary 2. Sum of percentages top 10 CDR3 clonotypes tumors p=0.01). 3. RT-PCR results for CTLA4, PD-1, IDO1, PD-L1, PD-L2, CD206 and FOXP3/CD4 ratio U-test). 4. DI, recurrence-free survival overall Kaplan-Meier curves. 5. T-stage, N-stage tumor localization U- test). 6. Significant...
<div>Abstract<p><b>Purpose:</b> Squamous cell carcinoma of the head and neck (SCCHN) is a lethal cancer with suboptimal 5-year overall survival approximately 50% surgery and/or definitive chemoradiotherapy. Novel treatments are thus urgently awaited. Immunotherapy checkpoint blockade has emerged as promising option for patients recurrent/metastatic SCCHN; however, it not been investigated in curative-intent setting yet. The purpose this study was to investigate T-cell...
<div>Abstract<p><b>Purpose:</b> Squamous cell carcinoma of the head and neck (SCCHN) is a lethal cancer with suboptimal 5-year overall survival approximately 50% surgery and/or definitive chemoradiotherapy. Novel treatments are thus urgently awaited. Immunotherapy checkpoint blockade has emerged as promising option for patients recurrent/metastatic SCCHN; however, it not been investigated in curative-intent setting yet. The purpose this study was to investigate T-cell...
<p>Supplementary Figure 1. TCR-β DI in HPV-positive patients (excluding smokers with {greater than or equal to}10packyear history) versus HPV-negative <10pack-year (Mann-Whitney U-test, p=0.004). Supplementary 2. Sum of percentages top 10 CDR3 clonotypes tumors p=0.01). 3. RT-PCR results for CTLA4, PD-1, IDO1, PD-L1, PD-L2, CD206 and FOXP3/CD4 ratio U-test). 4. DI, recurrence-free survival overall Kaplan-Meier curves. 5. T-stage, N-stage tumor localization U- test). 6. Significant...
Abstract Cytotoxic T lymphocytes (CTLs) play critical roles in cancer-immune responses, and functional characterization of CTLs their cancer-specific antigens will facilitate cancer immunotherapies. Immunogenic peptides, which can be derived from oncogenic proteins specifically expressed cells but not normal organs except testis (oncoantigens), or peptides with somatic nonsynonymous mutations (neoantigens), are known as good targets for to eradicate cells. In this study, we aimed establish a...
Abstract While multiple post-translational modifications have been reported to regulate the function of epidermal growth factor receptor (EGFR), effect protein methylation on its has not well characterized. In this study, we show that WHSC1L1 mono-methylates lysine 721 in tyrosine kinase domain EGFR, and leads enhanced activation downstream ERK cascade without EGF stimulation. We also EGFR K721 mono-methylation only affects cytoplasmic but nuclear EGFR. WHSC1L1-mediated nucleus interaction...
3080 Background: Immunotherapy with checkpoint blockade was recently approved for patients recurrent/metastatic SCCHN, however it has not been investigated in the curative-intent setting yet. In this study, we T-cell receptor repertoire and immune microenvironment tumor tissues of SCCHN locoregionally advanced disease. Methods: sequencing polymerase chain reaction immune-related genes from 44 prior to treatment definitive chemoradiotherapy were conducted. clonality mRNA expression levels...