A5029785146- Rheumatoid Arthritis Research and Therapies
- Systemic Lupus Erythematosus Research
- Monoclonal and Polyclonal Antibodies Research
- Autoimmune and Inflammatory Disorders Research
- Cell Adhesion Molecules Research
- Immune Cell Function and Interaction
- SARS-CoV-2 and COVID-19 Research
- Bone Metabolism and Diseases
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Chronic Lymphocytic Leukemia Research
- COVID-19 Clinical Research Studies
- Eosinophilic Disorders and Syndromes
- T-cell and B-cell Immunology
- Viral-associated cancers and disorders
- Foot and Ankle Surgery
- Lymphoma Diagnosis and Treatment
- Galectins and Cancer Biology
- Vasculitis and related conditions
- Bone and Dental Protein Studies
- Cytokine Signaling Pathways and Interactions
- Glycosylation and Glycoproteins Research
- Spondyloarthritis Studies and Treatments
- Salivary Gland Tumors Diagnosis and Treatment
- Gut microbiota and health
- Cytomegalovirus and herpesvirus research
Osaka Minami Medical Center
2016-2025
Nara Medical University
2003-2022
Kyushu University
2022
Kyushu University Beppu Hospital
2022
Keio University
2022
Hoshigaoka Medical Center
2022
Shimane University Hospital
2022
University of Michigan–Ann Arbor
2022
Kansai Medical University
2022
National Hospital Organization
2013-2015
To investigate the direct role of interleukin (IL) 6 in development rheumatoid arthritis, IL-6-deficient (IL-6 −/−) mice were backcrossed for eight generations into C57BL/6 mice, a strain with genetic background susceptibility antigen-induced arthritis (AIA). Both histological and immunological comparisons made between wild-type +/+) littermates after induction AIA. Although all IL-6 +/+ developed severe only mild was observed −/− mice. Safranin O staining demonstrated that articular...
Abstract Objective To characterize the expression pattern and role of galectin 3 binding protein (G3BP) in rheumatoid arthritis (RA), comparison with 1, to explore whether soluble G3BP, investigated serum, synovial fluid, or cell culture supernatant, are associated disease. Methods Synovial tissues from patients RA osteoarthritis (OA), as well healthy controls, were analyzed for galectins 1 G3BP by situ hybridization immunohistochemistry. Levels serum fluid OA supernatants, determined...
Objective To investigate the roles of interleukin-6 (IL-6) in pathogenesis rheumatoid arthritis (RA) by studying its effect on murine collagen-induced (CIA). Methods IL-6–deficient (IL-6−/−) mice with a genetic background susceptibility to CIA were generated backcrossing them DBA/1J for 8 generations. Clinical and immunologic features compared between these IL-6 wild-type (IL-6+/+) littermates CIA. Results Serum levels increased during development IL-6+/+ mice. Two prominent peaks observed....
To identify initial predictors of poor survival in patients with PM/DM-associated interstitial lung disease (ILD).We established a multicentre retrospective cohort incident cases ILD from 44 institutions across Japan (Multicentre Retrospective Cohort Japanese Patients Myositis-associated ILD, JAMI). Inclusion criteria were an onset age ⩾16 years; PM/DM or clinically amyopathic DM according to the published criteria; imaging evidence ILD; and availability serum samples for assays...
Objective Alteration of the gut microbiome has been linked to pathogenesis systemic lupus erythematosus (SLE). However, a comprehensive view in SLE and its interaction with host remains be revealed. This study aimed reveal SLE-associated changes by metagenome-wide association (MWAS) followed integrative analysis. Methods We performed MWAS based on shotgun sequencing microbial DNA from Japanese individuals ( N case =47, control =203). integrated result genome-wide (GWAS) data plasma...
Abstract Human DNA present in faecal samples can result a small number of human reads gut shotgun metagenomic sequencing data. However, it is presently unclear how much personal information be reconstructed from such reads, and this has not been quantitatively evaluated. Such quantitative evaluation necessary to clarify the ethical concerns related data sharing enable efficient use genetic stool samples, as for research forensics. Here we used genomic approaches reconstruct metagenomes 343...
Aberrant immune responses to viral pathogens contribute pathogenesis, but our understanding of pathological caused by viruses within the human virome, especially at a population scale, remains limited. We analyzed whole-genome sequencing datasets 6,321 Japanese individuals, including patients with autoimmune diseases (psoriasis vulgaris, rheumatoid arthritis (RA), systemic lupus erythematosus (SLE), pulmonary alveolar proteinosis (PAP) or multiple sclerosis) and coronavirus disease 2019...
<i>Background:</i> We have previously shown that fisetin, a flavonol, inhibits IL-4 and IL-13 synthesis by allergen- or anti-IgE-antibody-stimulated basophils. This time, we investigated the inhibition of production basophils other flavonoids attempted to determine fundamental structure related inhibition. additionally whether suppress leukotriene C4 T cells in response anti-CD3 antibody. <i>Methods:</i> Highly purified peripheral were stimulated for 12 h with...
Abstract Mouse milk fat globule epidermal growth factor 8 (MFG-E8), which is secreted by a subset of activated macrophages, binds to apoptotic cells recognizing phosphatidylserine and promotes their engulfment. Many are left unengulfed in the germinal centers spleen MFG-E8−/− mice, these mice develop an autoimmune disease resembling human systemic lupus erythematosus (hSLE). Here, we report that hMFG-E8 bound integrin αvβ3 complex. Increasing concentrations MFG-E8 generated bell-shaped...
Objective The relationship between autoimmune diseases and the gut microbiome has been intensively studied, several autoimmunity-associated bacterial taxa have identified. However, much less is known about roles of virome in diseases. Methods Here, we performed a whole analysis based on shotgun sequencing 476 Japanese which included patients with rheumatoid arthritis (RA), systemic lupus erythematosus (SLE), multiple sclerosis healthy control subjects. Results Our case–control comparison...
Abstract Background Interstitial lung disease (ILD) related to rheumatoid arthritis (RA) is among the leading causes of death and an essential prognostic factor. There only limited evidence for safety anti-rheumatic drugs patients with RA-ILD. The aim this study investigate efficacy Janus kinase inhibitors (JAKis) by comparing it abatacept (ABT) in Methods This single centre, retrospective nested case–control enrolled RA-ILD treated JAKi or ABT. To determine two existing ILD, we compared...
We have identified the genes whose expressions are augmented in blood cells of patients with systemic lupus erythematosus (SLE) using 'stepwise subtraction' technique along microarray analysis. The expression levels these were assessed by quantitative real-time reverse transcription polymerase chain reaction (RT-PCR) 31 SLE and 30 healthy controls. found that following eight significantly increased patients; interferon (IFN)-alpha-inducible protein 27 (IFI27), IFN-alpha-inducible IFI-15K...
We showed that CD9, a member of tetraspanin superfamily proteins, is expressed in specific membrane microdomain, called "lipid raft," and crucial for cell fusion during osteoclastogenesis after activation the RANK/RANKL system. Introduction: Osteoclasts are bone-resorbing multinuclear polykaryons essential bone remodeling formed through mononuclear macrophage/monocyte lineage precursors. Although has been shown to be critically regulated by system, mechanism how precursor cells fuse with...
Abstract Polyarteritis nodosa (PAN) is systemic vasculitis, typically treated with a combination of glucocorticoids and immunosuppressants. Changing the immunosuppressant recommended if remission cannot be achieved these treatments. However, there lack further treatment options for patients who are unresponsive to all We report 44-year-old Japanese man refractory PAN was various immunosuppressants (including tocilizumab). Upadacitinib led relatively rapid symptom improvement, allowing...
Profiling of oligosaccharide structures is widely utilized for both identification and evaluation glycobiomarkers, site-specific profiling N-linked glycans glycoproteins conducted by mass spectrometry glycopeptides. However, our knowledge mucin-type O-glycans including site occupancy profile variance, as well attachment sites, quite limited. Saccharide compositions site-occupancy were calculated from the signal intensity glycopeptide ions in spectra tandem electron transfer dissociation. The...
The Japanese National Hospital Organization evidence-based medicine (EBM) Study group for Adverse effects of Corticosteroid therapy (J-NHOSAC) is a hospital-based cohort study investigating the safety initial use glucocorticoids (GCs) in patients with newly diagnosed autoimmune diseases. Using J-NHOSAC registry, purpose this observational to analyse rates, characteristics and associated risk factors intracellular infections diseases who were initially treated GCs.A total 604 GCs enrolled...
Based on our previous results, which pointed to tumor necrosis factor-alpha (TNF-alpha) as the essential cytokine in promotion mouse skin, we present here three principal findings related specific roles of TNF-alpha, interleukin-1 (IL-1) and IL-6 (using TNF-alpha- IL-6-deficient mice) BALB/3T3 cell transformation: i) The previously reported residual by 12-O-tetradecanoylphorbol-13-acetate (TPA) TNF-/- mice was confirmed experiments with TNF+/+ 129/Svj same strain, using two-stage...
We showed that RGS18, a myeloid lineage-specific RGS protein is inhibited after activation of the RANK/RANKL system, negative regulator osteoclastogenesis. RGS18 acts through an external acidosis-sensing osteoclastogenic mechanism OGR1/NFAT pathway.Osteoclasts are bone-resorbing multinuclear giant cells differentiated from mononuclear macrophage/monocyte lineage precursors stimulated by system. The regulators G-protein signaling (RGS) family diverse group proteins accelerate intrinsic GTP...
Understanding the genetic effects on non-coding RNA (ncRNA) expression facilitates functional characterization of disease-associated loci. Among several classes ncRNAs, microRNAs (miRNAs) are key post-transcriptional gene regulators. Despite its biological importance, previous studies architecture miRNA focused mostly European individuals, underrepresented in other populations. Here, we mapped quantitative trait loci (miRNA-eQTL) for 343 miRNAs 141 Japanese using small sequencing and...
Many patients with severe COVID-19 suffer from pneumonia and the elucidation of mechanisms underlying development this condition is important. The in vivo function ORF8 protein secreted by SARS-CoV-2 not well understood. Here, we analyzed generating ORF8-knockout found that lung inflammation observed wild-type SARS-CoV-2-infected hamsters was decreased hamsters. Administration recombinant to also induced lymphocyte infiltration into lungs. Similar pro-inflammatory cytokine production primary...