A5080062736- Autoimmune Neurological Disorders and Treatments
- Myasthenia Gravis and Thymoma
- Peripheral Neuropathies and Disorders
- Cancer Immunotherapy and Biomarkers
- Cardiovascular Syncope and Autonomic Disorders
- Immunotherapy and Immune Responses
- Immune cells in cancer
- Genetic Neurodegenerative Diseases
- RNA Interference and Gene Delivery
- Monoclonal and Polyclonal Antibodies Research
- Immune Cell Function and Interaction
- CAR-T cell therapy research
- CNS Lymphoma Diagnosis and Treatment
- Lymphoma Diagnosis and Treatment
- Ion channel regulation and function
- Nicotinic Acetylcholine Receptors Study
- Cancer Cells and Metastasis
- Alzheimer's disease research and treatments
- Cell Adhesion Molecules Research
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Antifungal resistance and susceptibility
- Neuroinflammation and Neurodegeneration Mechanisms
- T-cell and B-cell Immunology
- Parkinson's Disease Mechanisms and Treatments
- Cancer Research and Treatments
Kumamoto University
2014-2023
Kumamoto City Hospital
2021-2022
Japan Science and Technology Agency
2011-2014
Center for Responsible Travel
2014
Centre de Recherche en Économie et Statistique
2014
Centre for Research in Engineering Surface Technology
2014
California Institute of Technology
2010
The University of Tokyo
2010
Abstract The aim of this study was to evaluate the association between postural orthostatic tachycardia syndrome ( POTS ) and circulating antiganglionic acetylcholine receptor gAChR antibodies. We reviewed clinical assessments Japanese patients with , determined presence antibodies in serum samples from those patients. Luciferase immunoprecipitation systems detected anti‐ α 3 β 4 sera (29%). Antecedent infections were frequently reported Moreover, autoimmune markers comorbid diseases also...
The purpose of this study was to evaluate the therapeutic potential human induced pluripotent stem (iPS) cell-derived macrophage-like cells for Alzheimer's disease (AD). In previous studies, we established technology generate myeloid lineage with proliferating capacity from iPS cells, and designated iPS-ML. iPS-ML reduced level Aβ added into culture medium, supernatant alleviated neurotoxicity Aβ. We generated expressing Fc-receptor-fused form a single chain antibody specific addition, made...
We recently developed a method to generate myeloid cells with proliferation capacity from human iPS cells. iPS-ML (iPS-cell–derived myeloid/macrophage line), generated by introducing and anti-senescence factors into iPS-cell–derived cells, grew continuously in an M-CSF–dependent manner. A large number of exhibiting macrophage-like properties can be readily obtained using this technology. In the current study, we evaluated possible application anti-cancer therapy. established model...
Abstract The purposes of the present study were to identify anti-striational antibodies in myasthenia gravis (MG) patients with myositis and/or myocarditis using a combination cell-based assays and flow cytometry (cytometric assays) describe main clinical implications. Among 2,609 stored samples collected from all over Japan between 2003 2016, we had serum 30 MG myocarditis. Cytometric titin, ryanodine receptor, voltage-gated Kv1.4 performed. Autoantibodies determined by differences...
Abstract Immune checkpoint inhibitors sometimes cause neuromuscular adverse events. Although a few cases of myasthenia gravis with hyper CK emia triggered by immune have been described, conclusive evidence remains limited. We conducted systematic review published related to inhibitors. Moreover, we tested anti‐striational antibodies in the case myositis after nivolumab administration. located 17 reports. Anti‐striational were six and five positive. Our analyses revealed poor prognosis combined
Background: Anti-programmed cell death 1 (PD-1) monoclonal antibodies (Abs) unleash an immune response to cancer. However, a disruption of the checkpoint function by blocking PD-1/PD-ligand 1(PD-L1) signaling may trigger myasthenia gravis (MG) as life-threatening immune-related adverse event. MG is neuromuscular disease and closely associated with being positive for anti-acetylcholine receptor (anti-AChR) Abs, which are high specific diagnostic Abs MG. Methods: A 72-year-old man was...
Abstract We established a method to generate large quantity of myeloid lineage cells from mouse embryonic stem (ES) cells, termed ES cell–derived proliferating cell lines (ES-ML). ES-ML continuously proliferated in the presence M-CSF and GM-CSF. genetically modified express an anti-HER2 (neu) mAb single-chain V region fragment reduced number cocultured Colon-26 cancer expressing HER2. Stimulation with IFN-γ plus LPS or TNF resulted almost complete killing by ES-ML, cytotoxicity was mediated,...
We established a method to produce large quantity of myeloid cells from human inducible pluripotent stem (iPSCs). When injected intraperitoneally into mice carrying peritoneal tumors, iPSC-derived (iPS-MCs) efficiently accumulated within neoplastic lesions. The intraperitoneal injection iPS-MCs expressing interferon β significantly inhibited the growth gastric and pancreatic cancers implanted in cavity immunocompromised mice.
We hypothesized that tissue-resident macrophages in familial amyloid polyneuropathy (FAP) patients will exhibit qualitative or quantitative abnormalities, may accelerate transthyretin (TTR)-derived deposition. To evaluate this, we examined the number and subset of heart tissue from amyloid-deposited FAP control patients. In both patients, were all Iba+/CD163+/CD206+ macrophages. However, was significantly decreased compared with Furthermore, proportion intracellular TTR CD14+ monocytes...
We herein demonstrate the immune-regulatory effect of embryonic stem cell-derived dendritic cells (ES-DCs) using two models autoimmune disease, namely non-obese diabetic (NOD) mice and experimental encephalomyelitis (EAE). Treatment pre-diabetic NOD with ES-DCs exerted almost complete suppression diabetes development during observation period for more than 40 weeks. The prevention by was accompanied significant reduction insulitis decreased number Th1 Th17 in spleen. Development EAE also...
Abstract We used an enzyme‐linked immunosorbent assay to measure pretreatment B cell‐activating factor belonging the tumour necrosis family ( BAFF ) and transmembrane activator CAML ‐interactor TACI levels in CSF serum collected from patients with primary central nervous system lymphoma PCNSL control groups. The decision tree analysis of for a diagnosis showed 100% sensitivity specificity when we attempted differentiate glioblastoma CNS inflammatory diseases. combination may thus be novel...
We aimed to establish a novel murine model of autoimmune autonomic ganglionopathy (AAG), which represents dysautonomia, associated with MHC class II understand its pathomechanism and the pathogenicity nicotinic acetylcholine receptor (nAChR) antibodies. The amino acid sequence mouse nAChRα3 protein was analyzed using an epitope prediction tool predict possible binding peptides. focused on two peptides in extracellular region, experimental AAG (EAAG) induced by immunization C57BL/6 mice these...
Abstract Objective Patients with acquired autonomic dysfunction may have antibodies specific to the ganglionic nicotinic acetylcholine receptor (gAChR). However, clinical features of children and adolescents (AAD) remain unclear. This study aimed determine pediatric patients dysfunction. Methods retrospectively examined a series AAD serum gAChR who were referred our laboratory for antibody testing between January 2012 April 2019. The included 200 (<20 years, 20 cases; ≥20 175 cases) AAD....
•We describe the first case of binasal hemianopia due to bilateral optic perineuritis.•Bilateral perineuritis should be considered as a causative disease hemianopia.•Early diagnosis is crucial avoid irreversible visual impairment.
Abstract Autoimmune encephalitis represented by anti‐NMDA receptor antibody positive is a type of nonherpetic limbic encephalitis, and it well known that emotional memory disorders manifest as symptoms. However, should be noted autonomic dysfunction also an important symptom system dysfunction. Autonomic manifestations autoimmune include not only general physical symptoms such high fever, sweating, bladder rectal disorders, but life‐threatening large fluctuations in blood pressure pulse,...