A5029224222- Neuroscience and Neuropharmacology Research
- MicroRNA in disease regulation
- Neuroinflammation and Neurodegeneration Mechanisms
- Circular RNAs in diseases
- Peroxisome Proliferator-Activated Receptors
- Medicinal Plants and Neuroprotection
- Signaling Pathways in Disease
- Zebrafish Biomedical Research Applications
- Axon Guidance and Neuronal Signaling
- Mitochondrial Function and Pathology
- Memory and Neural Mechanisms
- Cancer-related molecular mechanisms research
- Nicotinic Acetylcholine Receptors Study
- RNA Research and Splicing
- Ion channel regulation and function
- Advanced Memory and Neural Computing
- Epilepsy research and treatment
- Domain Adaptation and Few-Shot Learning
- Amino Acid Enzymes and Metabolism
- Congenital heart defects research
- Medicinal Plants and Bioactive Compounds
- RNA Interference and Gene Delivery
- Genetics and Neurodevelopmental Disorders
- Advanced biosensing and bioanalysis techniques
- Hippo pathway signaling and YAP/TAZ
Instytut Biologii Doświadczalnej im. Marcelego Nenckiego
2006-2024
Polish Academy of Sciences
2005-2019
Poznan University of Medical Sciences
2016
Toronto Western Hospital
2010-2015
University of Toronto
2010-2011
To promote functional recovery after CNS injuries, it is crucial to develop strategies that enhance both neuronal survival and regeneration. Here, we report caspase-6 upregulated in injured retinal ganglion cells its inhibition promotes regeneration these adult neurons. Treatment of rat whole mounts with Z-VEID-FMK, a selective inhibitor caspase-6, enhanced cell survival. Moreover, explants treated this drug extended neurites on myelin. We also show resulted improved robust axonal following...
Abstract Epilepsy frequently develops as a result of brain insult; however, there are no tools allowing to predict which patients suffering from trauma will eventually develop epilepsy. microRNAs interesting candidates for biomarkers, several them have been described change their levels in the brains, and plasma epileptic subjects. This study was conducted evaluate usefulness miRNAs epileptogenesis/epilepsy biomarkers. In our studies, we used rat model temporal lobe An epileptogenic insult...
VPS10P domain receptors emerge as central regulators of intracellular protein sorting in neurons with relevance for various brain pathologies. Here, we identified a role the family member SorCS2 protection from oxidative stress and epilepsy-induced cell death. We show that acts receptor sustains surface expression neuronal amino acid transporter EAAT3 to facilitate import cysteine, required synthesis reactive oxygen species scavenger glutathione. Lack causes depletion plasma membrane impairs...
J. Neurochem. (2010) 113 , 904–918. Abstract Although, astrocytes are more resistant than neurons to ischemic injury, astrocyte death has been demonstrated in animal models of brain ischemia. Astrocytes after ischemia/reperfusion may strongly affect neuronal survival because the absence their trophic and metabolic support neurons, astrocytic glutamate uptake. Early signals involved poorly understood. We enhanced mostly cytoplasmic activation extracellular signal‐regulated kinases 1 2...
The Morris Water Maze is commonly used in behavioural neuroscience for the study of spatial learning with rodents. Over years, various methods analysing rodent data collected this task have been proposed. These span from classical performance measurements (e.g. escape latency, speed, quadrant preference) to more sophisticated categorisation which classify animal swimming path into classes known as strategies. Classification techniques provide additional insight relation actual behaviours but...
Neuron-astrocyte interactions are critical for signalling, energy metabolism, extracellular ion and glutamate homeostasis, volume regulation neuroprotection in the CNS. Glutamate uptake by astrocytes may prevent excitotoxic elevation determine neuronal survival. However, an excess of can cause death astrocytes. FK506, inhibitor calcineurin, immunosuppressive drug, is neuroprotective animal models neurologic diseases, including focal global ischaemia. In present work, we demonstrate that a...
The present study performed a detailed analysis of behavior in rat model epilepsy using both established and novel methodologies to identify behavioral impairments that may differentiate between animals with short versus long latency spontaneous seizures low high number seizures. Temporal lobe was induced by electrical stimulation the amygdala. Rats were stimulated for 25 min 100-ms trains 1-ms biphasic square-wave pluses delivered every 0.5 s. Electroencephalographic recordings classify...
Abstract The dependence receptor Neogenin and its ligand, the repulsive guidance molecule a (RGMa), regulate apoptosis axonal growth in developing adult central nervous system (CNS). Here, we show that this pathway has also critical role neuronal death following stroke, providing RGMa to neurons blocks Neogenin-induced death. Interestingly, pro-death function ischemic insult depends on association with lipid rafts. Thus, peptide prevents rafts increased survival several vitro stroke models....
The goal of this study was to determine whether a substantial decrease in adult neurogenesis influences epileptogenesis evoked by the intra-amygdala injection kainic acid (KA). Cyclin D2 knockout (cD2 KO) mice, which lack almost entirely, were used as model. First, we examined status epilepticus (SE) an KA induces cell proliferation cD2 KO mice. On day after SE, injected BrdU into mice for 5 days and evaluated number DCX- DCX/BrdU-immunopositive cells 3 later. In control animals, only small...
Abstract Epilepsy frequently develops as a result of brain insult, for example, injury or stroke. Currently, there are no tools allowing us to predict which trauma patients will eventually develop epilepsy. There is evidence that microRNAs levels altered in the blood, making them attractive candidates peripheral biomarkers We analyzed white blood cell subpopulations containing miR-155-5p and miR-674-3p, control stimulated animals symptomatic asymptomatic amygdala stimulation model. The first...
Abstract Objective Structural epilepsies can manifest months or years after the occurrence of an initial epileptogenic insult, making them amenable for secondary prevention. However, development preventive treatments has been challenged by a lack biomarkers identifying subset individuals with highest risk epilepsy insult. Methods Four different rat models epileptogenesis were investigated to identify differentially expressed circulating microRNA (miRNA) and isomiR profiles as...
Abstract Background Epilepsy frequently develops as a result of brain insult, e.g., injury or stroke; however, currently, there are no tools allowing us to predict which patients suffering from trauma will eventually develop epilepsy. microRNAs interesting candidates for biomarkers, several them have been described change their levels in the brains epileptic subjects. There is evidence suggesting that microRNA also altered plasma during This study was conducted evaluate usefulness miRNAs...
Abstract Methyl CpG binding domain 3 (Mbd3) protein belongs to the MBD family of proteins and is responsible for reading DNA methylation pattern. Our previous study showed increased levels NuRD complex proteins, including Mbd3 protein, in brains epileptic animals. The present investigated whether determines seizure threshold. An increase was demonstrated entorhinal cortex/amygdala rat’s brain 4 hours after pentylenetetrazole (PTZ)-induced seizures. Reduction level using shRNA coding AAV...
SUMMARY The family of VPS10P domain receptors emerges as central regulator intracellular protein sorting in neurons with relevance for various brain pathologies. Here, we identified a unique role the member SorCS2 protection from oxidative stress and epilepsy-induced cell death. We show that acts receptor targets neuronal amino acid transporter EAAT3 to plasma membrane facilitate import cysteine, required synthesis reactive oxygen species scavenger glutathione. Absence activity causes...