A5100427777- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Cancer-related molecular mechanisms research
- Immune cells in cancer
- Circular RNAs in diseases
- Liver physiology and pathology
- Extracellular vesicles in disease
- Osteoarthritis Treatment and Mechanisms
- Immunotherapy and Immune Responses
- Adipose Tissue and Metabolism
- RNA modifications and cancer
- Metal complexes synthesis and properties
- Immune Cell Function and Interaction
- Mesenchymal stem cell research
- NF-κB Signaling Pathways
- Cancer Cells and Metastasis
- Pancreatic function and diabetes
- Bone and Joint Diseases
- Cytokine Signaling Pathways and Interactions
- Ferrocene Chemistry and Applications
- Wound Healing and Treatments
- Advanced biosensing and bioanalysis techniques
- Nanoparticle-Based Drug Delivery
- Fibroblast Growth Factor Research
- Orthopaedic implants and arthroplasty
Nanjing University
2016-2025
Center for Disease Control
2025
Ocean University of China
2024
State Key Laboratory of Pharmaceutical Biotechnology
2010-2022
Pharmaceutical Biotechnology (Czechia)
2013-2022
China Pharmaceutical University
2009-2022
Nanjing Medical University
2021
Shanxi Medical University
2012-2019
Nanjing Drum Tower Hospital
2019
Nanjing Tech University
2006-2018
Cell-derived exosomes have been demonstrated to be efficient carriers of small RNAs neighbouring or distant cells, highlighting the preponderance as for gene therapy over other artificial delivery tools. In present study, we employed modified expressing neuron-specific rabies viral glycoprotein (RVG) peptide on membrane surface deliver opioid receptor mu (MOR) siRNA into brain treat morphine addiction. We found that MOR could efficiently packaged RVG and was associated with argonaute 2...
BACKGROUND Although microRNAs (miRNAs) play essential roles in spermatogenesis, little is known about seminal plasma miRNAs infertile men. We investigated the profile of men to identify that are altered infertility; we then evaluated their diagnostic value. METHODS Seminal samples were obtained from 289 and 168 age-matched fertile control individuals. The stability was first assessed by time-course freeze–thaw cycle analyses. Solexa sequencing technology used for an initial screen pooled 45...
Switching macrophages from a pro-tumor type to an anti-tumor state is promising strategy for cancer immunotherapy. Existing agents, many derived bacterial components, have safety or specificity concerns. Here, we postulate that the structures of signals can be mimicked by using non-toxic biomolecules simple design. Based on bioactivity screening, devise glucomannan polysaccharide with acetyl modification at degree 1.8 (acGM-1.8), which specifically activates toll-like receptor 2 (TLR2)...
Abstract The CRISPR-Cas9 system offers substantial potential for cancer therapy by enabling precise manipulation of key genes involved in tumorigenesis and immune response. Despite its promise, the faces critical challenges, including preservation cell viability post-editing ensuring safe vivo delivery. To address these issues, this study develops an targeting tumor-associated macrophages (TAMs). We employ bacterial protoplast-derived nanovesicles (NVs) modified with pH-responsive...
The involvement of circulating microRNAs (miRNAs) in cancer and their potential as biomarkers diagnosis prognosis are becoming increasingly appreciated; however, little is known about miRNA profiles astrocytomas. In our study, we performed genome-wide serum analysis by the Solexa sequencing followed validation conducted training verification sets with a stem-loop quantitative reverse-transcription PCR (RT-qPCR) assay from samples 122 untreated astrocytomas patients (WHO grades III-IV) 123...
Abstract Doxorubicin is one of the most effective chemotherapeutic agents used for cancer treatment, but it causes systemic inflammation and serious multiorgan side effects in many patients. In this study, we report that upregulation proinflammatory Toll-like receptor TLR4 macrophages by doxorubicin an important step generating its toxic effects. patient serum, treatment resulted leakage endotoxin inflammatory cytokines into circulation. mice, damaged intestinal epithelium, which also from...
Abstract Background microRNAs (miRNAs) are endogenous small non-coding RNAs that regulate gene expression at the post-transcriptional level. While number of known human and murine miRNAs is continuously increasing, information regarding from other species such as amphioxus remains limited. Results We combined Solexa sequencing with computational techniques to identify novel in B. belcheri (Gray). This approach allowed us 113 miRNA genes. Among them, 55 were conserved across encoded 45...
Transforming growth factor-β (TGFβ) is crucial for liver fibrogenesis and the blunting of TGFβ signalling in hepatic stellate cells (HSCs) or hepatocytes can effectively inhibit fibrosis. microRNAs (miRNAs) have emerged as key regulators modulating fibrogenesis. However, regulation receptor I (TβRI) production by miRNA remains poorly understood. Here we demonstrate that miR-101 family members act suppressors targeting TβRI its transcriptional activator Kruppel-like factor 6 (KLF6) during...
Strategies to modify nanoparticles with biological ligands for targeted drug delivery in vivo have been widely studied but met limited clinical success. A possible reason is that, the blood circulation, serum proteins could rapidly form a layer of protein "corona" on vehicle surface, which might block modified and hamper their targeting functions. We speculate that strategies can be designed based upon elegant control corona formation surfaces. In this study, we demonstrate...
Cell-derived exosomes are leading candidates for in vivo drug delivery carriers. In particular, derived from 293T cells used most frequently, although exosome dosing has varied greatly among studies. Considering their biological origin, it is crucial to characterize the molecular composition of if large doses be administered clinical settings. this study, we present first comprehensive analysis protein, messenger RNA and microRNA profiles cell-derived exosomes; then, characterized these data...
Wear particle-induced osteolysis is the leading cause of aseptic loosening, which most common reason for THA (total hip arthroplasty) failure and revision surgery. Although existing studies suggest that osteoblast apoptosis induced by wear debris involved in underlying mechanism linking particles to remains almost totally unknown. In present study, we investigated effect autophagy on CoCrMo metal (CoPs) vitro a calvarial resorption animal model. Our study demonstrated CoPs stimulated...