A5064322654- Zebrafish Biomedical Research Applications
- Radiation Therapy and Dosimetry
- Neurogenesis and neuroplasticity mechanisms
- MicroRNA in disease regulation
- Reproductive biology and impacts on aquatic species
- Cell death mechanisms and regulation
- Developmental Biology and Gene Regulation
- Fish Ecology and Management Studies
- Pluripotent Stem Cells Research
- Retinal Development and Disorders
- Sperm and Testicular Function
- Congenital heart defects research
- Cancer-related Molecular Pathways
- Effects of Radiation Exposure
- Skin Diseases and Diabetes
- Reproductive Biology and Fertility
- Smoking Behavior and Cessation
- Biocrusts and Microbial Ecology
- Physiological and biochemical adaptations
- Immune cells in cancer
- Voice and Speech Disorders
- Diatoms and Algae Research
- Renal and related cancers
- Microbial Community Ecology and Physiology
- Fish Biology and Ecology Studies
Tokyo University of Science
2025
The University of Tokyo
2012-2023
Japan Women's University
2022-2023
National Institute for Environmental Studies
2022
Frontier Science Foundation
2017
Hamamatsu University School of Medicine
2011-2013
Kobe University
2003-2010
Radiation Effects Research Foundation
2008
National Institutes for Quantum Science and Technology
2008
Divergent synthesis of triazoles was achieved using newly designed platform molecules possessing azide, alkyne, and fluorosulfonyl moieties. Consecutive conjugations by the sulfur–fluoride exchange following consecutive triazole formations allowed us...
Receptor for advanced glycation end products (RAGE) plays a pivotal role in the genesis of diabetic vascular diseases. To further explore mechanisms underlying atherosclerosis under non-diabetic conditions, we examined effect RAGE deficiency on hyperlipidaemic mice. RAGE−/− mice were crossed with low-density lipoprotein receptor-deficient (LDLr−/−) to generate double knockout (DKO) After feeding high-fat diet 12 weeks, aortic atherosclerotic lesions analysed histologically these Although...
We propose here a novel interpretation of the embryonic origin cells diencephalic sensory relay nuclei in teleosts based on our recent studies gene expression patterns medaka <i>(Oryzias latipes)</i> brain and comparative hodological studies. It has been proposed that system is unique among vertebrates. Teleost nuclei, preglomerular complex (PG), have assumed to originate from basal plate (the posterior tuberculum) diencephalon, whereas mammals are derived alar (dorsal thalamus)...
AimsIn addition to their cholesterol-lowering effect, statins increase high-density lipoprotein cholesterol (HDL-C) levels. Endothelial lipase (EL) is a regulator of plasma HDL-C In the present study, effects on EL expression were investigated.
Abstract We examined the morphogenesis and regionalization of embryonic brain an acanthopterygian teleost, medaka ( Oryzias latipes ), by in situ hybridization using 14 gene probes. compared our results with previous studies other vertebrates, particularly zebrafish, ostariophysan teleost. During early development neural rod, three initial vesicles arose: anterior vesicle, which later developed into telencephalon rostral diencephalon; intermediate caudal diencephalon, mesencephalon,...
In this study, the roles of p53 in impaired spermatogenic male germ cells p53-deficient medaka were investigated by analyzing histological changes, and gene expressions 42Sp50, Oct 4 vitellogenin (VTG2) RT-PCR or situ hybridization testes. We found that a small number oocyte-like (testis–ova) differentiated spontaneously cysts type A early B spermatogonia testes, contrast to wild-type (wt) testes which testis–ova never found. Furthermore, ionizing radiation (IR) irradiation increased size...
Abstract Aim To determine the postpartum smoking relapse rate among women in J apan who quit during pregnancy and to clarify factors related relapse. Methods A self‐administered questionnaire survey was conducted as a cross‐sectional study of all mothers children underwent health checkups after birth randomly selected municipalities from M ay uly 2009. Using valid data 20 601 mothers, calculated. In addition, χ 2 ‐test multiple logistic regression analysis were used Results The rates 15.8%...
To understand how humans adapt to the space environment, many experiments can be conducted on astronauts as they work aboard Space Shuttle or International Station (ISS). We also need animal that apply human models and help prevent solve health issues we face in travel. The Japanese medaka (Oryzias latipes) is a suitable model fish for studying adaptation evidenced by adults of species having mated successfully during 15 days flight second Microgravity Laboratory mission 1994. eggs laid...
Abstract In order to know the general pattern of axonogenesis in vertebrates, we examined embryonic brain a teleost fish, medaka ( Oryzias latipes ), and results were compared with previous studies zebrafish mouse. The axons somata stained immunocytochemically using antibodies cell surface marker (HNK‐1) acetylated tubulin visualized by retrograde anterograde labeling lipophilic dye. fiber systems developed correlating organization longitudinal transverse subdivisions brain. first extended...
Medaka (Oryzias latipes) embryos at 25-26 and 28-30 stages were irradiated with a single acute dose of 10 Gy X-ray, which is lower than the LD(50 )of embryos. The effects on developing brains examined under stereomicroscope in living until hatching. All survived; however, from 6 to 35 h after X-ray irradiation, massive clusters optically opaque round cells observed either entire brain region (when stages) or mainly optic tectum stages). Histological examination TUNEL showed that these are...
We describe a novel method for rapid and quantitative evaluation of the degree radiation-induced apoptosis in developing brain medaka (Oryzias latipes). Embryos at stage 28 were irradiated with 1, 2, 3.5, 5 Gy x-ray. Living embryos stained vital dye, acridine orange (AO), 1-2 h, whole-mount brains examined under an epifluorescence microscope. From 7 to 10 h after irradiation x-ray, we found two morphologically different types AO-stained structures, namely, small single nuclei rosette-shaped...
Abstract Radiotherapy is widely used in cancer treatment. In addition to inducing effects the irradiated area, irradiation may induce on tissues close and distant from area. Japanese medaka, Oryzias latipes , a small teleost fish model organism for evaluating environmental of radiation. this study, we applied low-energy carbon-ion (26.7 MeV/u) adult medaka depth approximately 2.2 mm body surface using an system at National Institutes Quantum Radiological Science Technology. We histologically...
Microglia remove apoptotic cells by phagocytosis when the central nervous system is injured in vertebrates. Ionizing irradiation (IR) induces apoptosis and microglial activation embryonic midbrain of medaka (Oryzias latipes), where apolipoprotein E (ApoE) upregulated later phase microglia In this study, we found that another marker, l-plastin (lymphocyte cytosolic protein 1), was at initial IR-induced activated changed their morphology increased motility to migrate. We further conducted...
The development of blood flow in the heart is crucial for function and embryonic survival. Recent studies have revealed importance extracellular matrix mechanical stress applied to valve cushion that controls formation cardiac during embryogenesis. However, events trigger such stress, their temperature dependence not been explained completely. Medaka (Oryzias latipes) inhabits a wide range East Asia adapts climates. We used medaka embryos from different genomic backgrounds analyzed heartbeat...
The cerebellar structures of teleosts are markedly different from those other vertebrates. cerebellum continues rostrally into the midbrain ventricle, forming valvula cerebelli, only in ray-finned fishes among To analyze ontogenetic processes that underlie this morphological difference, we examined early development regions, including isthmus (mid/hindbrain boundary, MHB), medaka (Oryzias latipes), by histology and in-situ hybridization using two gene (wnt1 fgf8) probes. Isthmic wnt1 was...
Radiation therapy (RT) is pivotal in the treatment of many central nervous system (CNS) pathologies; however, exposure to RT children associated with a higher risk secondary CNS tumors. Although recent research interest has focused on reparative and therapeutic role microglia, their recruitment following not been elucidated, especially developing CNS. Here, we investigated spatiotemporal dynamics microglia during tissue repair irradiated embryonic medaka brain by whole-mount situ...
To observe the sequential radiation-induced apoptosis in a living embryo, we injected Acridine Orange (AO) solution into yolk of embryo and visualized developing optic tectum (OT). Medaka embryos at stage 28, when neural cells proliferate rapidly OT, were irradiated with 5 Gy X-rays which is non-lethal dose for hatching. The hatched normally without morphological abnormalities their brains, even though large number apoptotic induced transiently OT. By injection, OT distinguished as...
We have examined cerebellar morphogenesis after neural tube stage in medaka (Oryzias latipes), a ray-finned fish, by conventional histology and immunohistochemistry using anti-proliferating cell nuclear antigen (PCNA) anti-acetylated tubulin antibodies. Our results indicate that the cerebellum is formed 4 successive stages: (1) formation enlargement of primordia; (2) rostral midline fusion left/right halves (3) matrix zones caudalmost regions primitive cerebellum, (4) growth differentiation...
Purpose: Exposure to heavy-ion radiation is considered a critical health risk on long-term space missions. The developing central nervous system (CNS) highly radiosensitive tissue; however, the biological effects of radiation, which are greater than those low-linear energy transfer (LET) not well studied, especially in vivo intact organisms. Here, we examined iron-ions CNS using vertebrate organism, fish embryos medaka (Oryzias latipes).Materials and methods: Medaka at developmental stage 28...
Eye development and brain structures of a mutant teleost fish were investigated. The el (eyeless) mutation in medaka (Oryzias latipes) is recessive affects eye formation; the most severe cases, it results absence eyes. Developmental studies revealed that normal eyeballs are not formed embryos, but small optic cup-like differentiate situ walls prosencephalon without evagination. anophthalmic homozygous hatched normally, although they did respond behaviorally to visual stimuli. A fraction...
Abstract Fatty acid‐binding proteins (FABPs) belong to a multigene family of small intracellular that bind hydrophobic ligands. Recent studies have indicated FABP7 plays important roles in neurogenesis or neuronal migration vertebrates. In this study, we isolated cDNA and the genomic fragment containing fabp7 gene for medaka fish examined expression through development their central nervous system (CNS). The consists four exons approximately 1 kb region. Its deduced amino acid sequence...