A5041401319- Glutathione Transferases and Polymorphisms
- Effects of Radiation Exposure
- Cancer-related gene regulation
- Traditional Chinese Medicine Analysis
- S100 Proteins and Annexins
- Viral Infectious Diseases and Gene Expression in Insects
- Virus-based gene therapy research
- Ubiquitin and proteasome pathways
- Cholinesterase and Neurodegenerative Diseases
- Genetic factors in colorectal cancer
- Burn Injury Management and Outcomes
- RNA Interference and Gene Delivery
- Genomics, phytochemicals, and oxidative stress
- Protein Interaction Studies and Fluorescence Analysis
- Biochemical and Structural Characterization
- Vitamin C and Antioxidants Research
- Ultrasound and Hyperthermia Applications
- Ginseng Biological Effects and Applications
- bioluminescence and chemiluminescence research
- Antimicrobial Peptides and Activities
- Advanced Drug Delivery Systems
- Cancer-related Molecular Pathways
- Microbial Natural Products and Biosynthesis
- Wnt/β-catenin signaling in development and cancer
- RNA Research and Splicing
Fuzhou University
2009-2024
The attractive potential of natural superoxide dismutase (SOD) in the fields medicine and functional food is limited by its short half-life circulation poor permeability across cell membrane. nanoparticle form SOD might overcome these limitations. However, most preparative methods have disadvantages, such as complicated operation, a variety reagents-some them even highly toxic-and low encapsulation efficiency or release rate. aim this study to present simple green approach for preparation...
Superoxide dismutase (SOD) fusion of TAT was proved to be radioprotective in our previous work. On that basis, a bifunctional recombinant protein which the glutathione S‐transferase (GST), SOD, and constructed named GST‐TAT‐SOD. Herein we report investigation cytotoxicity, cell‐penetrating activity, vitro effect GST‐TAT‐SOD compared with wild single‐function SOD‐TAT, amifostine. We demonstrated SOD had little on irradiated L‐02 Hep G2 cells while amifostine protective both cell lines....
Annexin A2 (ANXA2) has been found to be involved in cancer proliferation, metastasis and prognosis; however, its exact role nasopharyngeal carcinoma (NPC) radioresistance remains unknown. We that ANXA2 expression was correlated with prognosis NPC patients, longer overall survival patients low than those high expression. knockdown increased the radiosensitivity radioresistant cells, overexpression decreased cells. Knocking-down irradiation-induced apoptosis of induced G2/M phase arrest cells...
GST-TAT-SOD was the fusion of superoxide dismutase (SOD), cell-permeable peptide TAT, and glutathione-S-transferase (GST). It proved to be a potential selective radioprotector in vitro our previous work. This study evaluated vivo radioprotective activity against whole-body irradiation. We demonstrated that intraperitoneal injection 0.5 ml (2 kU/ml) 2 h before 6 Gy irradiation mice almost completely prevented splenic damage. could significantly enhance antioxidant which kept number white pulp...
Nasopharyngeal carcinoma (NPC) is rare worldwide but remains highly prevalent in endemic regions, notably southern China. Radiotherapy the treatment of choice for NPC, radioresistance has been identified as a major cause therapeutic failure. The Wnt/β-catenin signaling found to be involved NPC radioresistance; however, effect β-catenin overexpression on unknown until now. This study aimed examine impact radiosensitivity human CNE-2 cells.Immunohistochemistry was performed detect expression...
GST‐TAT‐SOD, a cell‐permeable bifunctional antioxidant enzyme, is potential selective radioprotector. This study aimed to investigate the cytoprotective activity of GST‐TAT‐SOD against cisplatin‐induced damage. The current showed that cisplatin induced formation reactive oxygen species in normal L‐02 cells. (2000 U/mL) executed its role by directly scavenging excess intracellular free radicals and augmenting cellular defense such as reducing MDA level, enhancing SOD activity, GST T‐AOC....
The superoxide dismutase (SOD) family of proteins are necessary to protect oxygen-utilizing cells from the toxicity reactive oxygen species. delivery SOD into tissues is severely limited by its size and biochemical properties. A cell-membrane-permeable SOD, SOD-TAT, has been demonstrated have ability be directly transduced mammalian cells. In this study, SOD-TAT fusion protein was expressed, purified crystallized. Crystals diffracted 3.20 Å resolution belonged space group C121.
<title>Abstract</title> <bold>Background:</bold> Colorectal cancer (CRC) is a significant public health issue, with metastatic disease posing considerable challenge despite therapeutic advancements. Panitumumab, an EGFR inhibitor, has been ray of hope for patients chemorefractory CRC. However, understanding the safety profile panitumumab crucial optimizing patient care. <bold>Methods:</bold> This study leveraged FDA's Adverse Event Reporting System (FAERS) to scrutinize ADR reports linked...
Superoxide dismutase (SOD) family is necessary to protect cells from the toxicity of reactive oxygen species produced during normal metabolism. Among SODs, manganese-containing superoxide (Mn-SOD, SOD2) most important one. The DNA fragment containing full nucleotide full-length human SOD2 was synthesized and inserted into prokaryotic expression vector pGEX-4T-1 with tag GST. construct then transformed Escherichia coli BL21 (DE3) induced IPTG at 25 ℃. recombinant fusion protein GST-SOD2 (46...
Background: Paraspeckle component 1 (PSPC1) is overexpressed in various cancer and correlated with poor survival the patients. However, little known about its expression role progression of nasopharyngeal carcinomas (NPC). The purpose this study to examine PSPC1 NPC explore clinical prognosis radiation therapy. Methods: association clinicopathological features 109 patients was examined using partial correlation analysis. Cancer tissues were obtained prior treatment. All cases diagnosed...
Antioxidant enzymes fused with cell-penetrating peptides could enter cells and protect from irradiation damage. However, the unselective transmembrane ability of peptide may also bring antioxidant into tumor cells, thus protecting consequently reducing efficacy radiotherapy. There are active matrix metalloproteinase (MMP)-2 or MMP-9 in most cellular microenvironments. Therefore, a fusion protein containing an MMP-2/9 cleavable substrate X, R9, glutathione S-transferase (GST), human Cu, Zn...
The fusion of cell permeable peptide TAT and bifunctional antioxidant enzymes, GST (Glutathione sulfur transferase)-TAT-SOD1 (Cu, Zn superoxide dismutase), is an intracellular scavenger. Compared with SOD1-TAT, GST-TAT-SOD1 has better protective effect on oxidative damage but less transduction efficiency. A novel enzymes the GST, SOD1 polyarginine R9 was constructed for higher full nucleotide sequence SOD1-R9 synthesized inserted into prokaryotic expression vector pGEX-4T-1 tag. After...
Two proteins of similar molecular weight (named as ASPR-C-1 and ASPR-C-2) from the crude drug Angelica sinensis were purified characterized by 80% ammonium sulfate precipitation, Sephadex G-50 gel filtration chromatography, DEAE-Sepharose anion exchange chromatography. The ASPR-C-2 on SDS-PAGE was 17.33 kDa 17.18 kDa, respectively. They mainly monomeric in solution, but partially formed dimers they glycoproteins with glycosyl content 2.6% 8.2%, Both identified to be members...