A5012128553- Influenza Virus Research Studies
- Viral gastroenteritis research and epidemiology
- Animal Disease Management and Epidemiology
- Animal Virus Infections Studies
- Salmonella and Campylobacter epidemiology
- Bacteriophages and microbial interactions
- Viral Infections and Vectors
- Virology and Viral Diseases
- Viral Infections and Immunology Research
- Genomics and Phylogenetic Studies
- Antimicrobial Resistance in Staphylococcus
- Respiratory viral infections research
- RNA and protein synthesis mechanisms
- Natural product bioactivities and synthesis
- Galectins and Cancer Biology
- Aquaculture disease management and microbiota
- Glycosylation and Glycoproteins Research
- Arsenic contamination and mitigation
- Virus-based gene therapy research
- SARS-CoV-2 and COVID-19 Research
- Bacterial biofilms and quorum sensing
- Escherichia coli research studies
- Antibiotic Resistance in Bacteria
- Microbial infections and disease research
- Digital Imaging in Medicine
Seoul National University
2014-2024
Kolmar Korea (South Korea)
2021-2022
University of Maryland, Baltimore
2021-2022
University of Maryland Medical Center
2022
National Institute of Biological Resources
2021
Korea National Institute of Health
2019
Green Technology Center
2017-2018
Animal Husbandry and Veterinary Department
2017-2018
National Institutes of Health
2014-2015
National Human Genome Research Institute
2015
ABSTRACT The swine hepatitis E virus (HEV) is considered to be a new zoonotic agent due its close genomic resemblance the human HEV and ability infect nonhuman primates. Hepatitis caused by infection has been serious public health problem in developing countries. However, recent seroprevalence studies indicate that also circulates industrialized In this study, nested reverse transcription (RT)-PCR was developed detect part of open reading frame 2. Three Korean isolates were identified 128...
A recombinant La Sota strain (KBNP-C4152R2L) in which fusion (F) and hemagglutinin-neuraminidase (HN) genes were replaced with those of a contemporary genotype VIId virus, KBNP-4152, has been developed. To attenuate the virulence strain, F cleavage motif was mutated from (112)RRQKR(116) to (112)GRQAR(116), reduce pathogenic instability, codon does not allow changes basic amino acids by single point mutation inserted at 115. In addition six-nucleotide sequence into intergenic region between...
Fifty-six Newcastle disease virus strains collected from 2000 to 2006 could be grouped into subgenotype VIId. However, they displayed cumulative mutations in and around the linear epitope of hemagglutinin-neuraminidase (residues 345 353) with time. The antigenicities variants that became predominant Korea differ each other wild type.
Background GM1 gangliosidosis is a lysosomal storage disorder caused by mutations in GLB1, encoding β‐galactosidase. The range of severity from type I infantile disease, lethal early childhood, to III adult onset, resulting gradually progressive neurological symtpoms adulthood. intermediate group patients has been recently classified as having II late subtype with onset symptoms at one three years age or juvenile symptom 2–10 years. To characterize disease and progression, six Late nine were...
Antibiograms and relevant genotypes of Korean avian pathogenic Escherichia coli (APEC) isolates (n = 101) recovered between 1985 2005 were assessed via disc diffusion test, PCR, restriction enzyme analysis, sequencing. These highly resistant to tetracycline (84.2%), streptomycin enrofloxacin (71.3%), ampicillin (67.3%), most the tetracycline, streptomycin, enrofloxacin, resistances associated with tetA and/or tetB, aadA strA-strB, mutations in gyrA parC, TEM, respectively. Class 1 integrons...
Abstract The PB2 gene is one of the key determinants for mammalian adaptation avian influenza A viruses (IAVs). Although pathogenicity-related mutations (MPMs) in genes were identified different genetic backgrounds IAVs, relative effects single or multiple on viral fitness could not be directly compared. Furthermore, their mutational steps during had been unclear. In this study, we collectively compared individual and combined MPMs determined rank orders using a prototypic gene. Early...
The first outbreak of low pathogenic avian influenza (LPAI), H9N2 virus subtype, in 1996 prompted an eradication response, but LPAI returned to Korea 1999. relationship between the and recurrent viruses is unclear. To determine molecular epizootiology LPAI, we performed phylogenetic analysis with partial nucleotide sequences four gene segments (HA, NA, NP PB2) from eight chicken-origin viruses. showed higher similarity haemagglutinin neuraminidase genes Korean isolates than other Eurasian...
ABSTRACT φSG-JL2 is a newly discovered lytic bacteriophage infecting Salmonella enterica serovar Gallinarum biovar but nonlytic to rough vaccine strain of (SG-9R), S. Enteritidis, Typhimurium, and Pullorum. The genome 38,815 bp in length (GC content, 50.9%; 230-bp-long direct terminal repeats), 55 putative genes may be transcribed from the same strand. Functions were assigned 30 based on high amino acid similarity known proteins. Most expected proteins except tail fiber (31.9%) overall...
The K-I and nephropathogenic K-II genotypes of infectious bronchitis virus (IBV) have been isolated since 1995 1990, respectively, in Korea commercial inactivated oil-emulsion vaccines containing KM91 (K-II type) Massachusetts 41 strains used the field. To date, genomic analyses Korean IBV animal models to test pathogenicity IBVs reproductive organs rare. In present study, comparative genomics SNU8067 (K-I was performed, an model established applied vaccine efficacy test. genome sizes...
Chimeric lysins composed of various combinations cell wall-lysing (enzymatic) and cell-wall-binding (CWB) domains endolysins, autolysins, bacteriocins have been developed as alternatives to or adjuvants conventional antibiotics. The screening multiple chimeric lysin candidates for activity via E. coli expression is not cost effective, we previously reported on a simple cell-free system an alternative. In this study, sufficiently improved upon use in turbidity reduction test, which more...
Fowl typhoid is a disease of adult chickens and caused by Salmonella Gallinarum infection via the alimentary tract. The experimental reproduction fowl per os (PO) requires artificial conditions to minimize effect gastric acid, several serovars have been known be transmitted respiratory route. Therefore, we hypothesized existence route for attempted reproduce intratracheal challenge. In accordance with our hypothesis, challenges reproduced exactly same lesions as induced higher mortality...
ABSTRACT A Salmonella lytic bacteriophage, SS3e, was isolated, and its genome sequenced completely. This phage is able to lyse not only various serovars but also Escherichia coli , Shigella sonnei Enterobacter cloacae Serratia marcescens indicating a broad host specificity. Genomic sequence analysis of SS3e revealed linear double-stranded DNA 40,793 bp harboring 58 open reading frames, which highly similar phages SETP13 MB78.
The polymerase of avian influenza A virus (AIV) is a heterotrimer composed PB2, PB1, and PA. PB2 plays role in overcoming the host barrier; however, genetic prerequisites for to acquire mammalian pathogenic mutations have not been well elucidated. Previously, we identified prototypic that conferred non-replicative non-pathogenic traits PR8-derived recombinant when it was used infect mice. Here, demonstrated key amino acid (I66M, I109V, I133V, collectively referred as MVV) this increase...