A5013406455- Gut microbiota and health
- Listeria monocytogenes in Food Safety
- Viral Infectious Diseases and Gene Expression in Insects
- Mycobacterium research and diagnosis
- Molecular Biology Techniques and Applications
- Vibrio bacteria research studies
- Cancer Immunotherapy and Biomarkers
- Advanced Electron Microscopy Techniques and Applications
- Tuberculosis Research and Epidemiology
- Microbial Metabolic Engineering and Bioproduction
- Tryptophan and brain disorders
- Cancer Research and Treatments
- Salmonella and Campylobacter epidemiology
- Advanced biosensing and bioanalysis techniques
- Escherichia coli research studies
- Brucella: diagnosis, epidemiology, treatment
- Diet and metabolism studies
- Animal Disease Management and Epidemiology
- Bacterial biofilms and quorum sensing
- Receptor Mechanisms and Signaling
- Metabolism and Genetic Disorders
- Leprosy Research and Treatment
- HIV-related health complications and treatments
- Bacteriophages and microbial interactions
- Anaerobic Digestion and Biogas Production
Bar-Ilan University
2024
Harvard University
2016-2022
Tel Aviv University
2012-2018
Associations between chronic kidney disease (CKD) and the gut microbiota have been postulated, yet questions remain about underlying mechanisms. In humans, dietary protein increases bacterial production of hydrogen sulfide (H2S), indole, indoxyl sulfate. The latter are uremic toxins, H2S has diverse physiological functions, some which mediated by posttranslational modification. a mouse model CKD, we found that high sulfur amino acid-containing diet resulted in posttranslationally modified...
Intracellular bacterial pathogens are metabolically adapted to grow within mammalian cells. While these adaptations fundamental the ability cause disease, we know little about relationship between pathogen's metabolism and virulence. Here used an integrative Metabolic Analysis Tool that combines transcriptome data with genome-scale metabolic models define requirements of Listeria monocytogenes during infection. Twelve pathways were identified as differentially active L. growth in macrophage...
Summary Metabolic adaptations are critical to the ability of bacterial pathogens grow within host cells and normally preceded by sensing host‐specific metabolic signals, which in turn can influence pathogen's virulence state. Previously, we reported that intracellular pathogen L isteria monocytogenes responds low availability branched‐chain amino acids ( BCAA s) mammalian up‐regulating both biosynthesis genes. The induction genes required ‐responsive transcription regulator, CodY , but...
Bacteria sense and respond to many environmental cues, rewiring their regulatory network facilitate adaptation new conditions/niches. Global transcription factors that co-regulate multiple pathways simultaneously are essential this rewiring. CodY is one such global regulator, controlling expression of both metabolic virulence genes in Gram-positive bacteria. Branch chained amino acids (BCAAs) serve as a ligand for modulate its activity. Classically, was considered function primarily...
The high environmental adaptability of bacteria is contingent upon their ability to sense changes in surroundings. Bacterial pathogen entry into host poses an abrupt and dramatic change, during which successful pathogens gauge multiple parameters that signal localization. facultative human Listeria monocytogenes flourishes soil, water food, ~50 different animals, serves as a model for intracellular infection. L. identifies by sensing both physical (e.g., temperature) chemical metabolite...
Listeria monocytogenes (Lm) is a saprophyte and intracellular pathogen. Transition to the pathogenic state relies on sensing of host-derived metabolites, yet it remains unclear how these are recognized they mediate virulence gene regulation. We previously found that low availability isoleucine signals Lm activate virulent state. This response dependent CodY, global regulator sensor. Isoleucine-bound CodY represses metabolic pathways including branched-chain amino acids (BCAA) biosynthesis,...
Abstract Objective Rapid, high-volume screening programs are needed as part of cervical cancer prevention in China. Methods In a 5-day project Inner Mongolia, 3345 women volunteered following community awareness campaign, and self-swabbed to permit rapid HPV testing. Two AmpFire™ detection systems (Atila Biosystems) were sufficient provide pooled 15-HPV type data within an hour. HPV+ patients had same-day digital colposcopy (DC) performed by 1 6 physicians, using the EVA™ system (MobileODT)....
Multi-drug resistance (MDR) transporters are known eponymously for their ability to confer various antimicrobial drugs. However, it is likely that this not primary function and MDR evolved originally play additional roles in bacterial physiology. In Listeria monocytogenes a set of was identified mediate activation innate immune responses during mammalian cell infection. This phenotype shown be dependent on c-di-AMP secretion, but the physiological processes underlying phenomenon were...
Listeria monocytogenes is an environmental saprophyte and intracellular bacterial pathogen. Upon invading mammalian cells, the bacterium senses abrupt changes in its metabolic environment, which are rapidly transduced to regulation of virulence gene expression. To explore relationship between L. metabolism virulence, we monitored expression dynamics across a library genetic mutants grown under two conditions known activate virulent state: charcoal-treated rich medium containing...
Studying the transcriptome of bacterial pathogens during infection is a very informative and effective tool for discovering genes that contribute to successful infection. However, isolating RNA from infected cells or tissues challenging process due much higher amounts host in lysates cells. We have optimized method Listeria monocytogenes (L. monocytogenes) bacteria infecting bone marrow derived macrophage (BMDM). After infection, we lyse filter through 0.45 µm filters discard most...
ABSTRACT We identify a novel mechanism linking diet, gut microbial metabolism, and renal function. found that sulfur amino acid-based dietary intervention post-translationally modifies enzyme, blunting its uremic toxin-producing activity alleviating chronic kidney disease (CKD) in preclinical model. also define heretofore unknown role for the post-translational modification S-sulfhydration within microbiome. This study provides framework understanding how diet can tune microbiota function...