A5079546270- Microfluidic and Bio-sensing Technologies
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Microbial Inactivation Methods
- Single-cell and spatial transcriptomics
- Intracranial Aneurysms: Treatment and Complications
- Gene expression and cancer classification
- Electrowetting and Microfluidic Technologies
- Cell Image Analysis Techniques
- Advanced Cellulose Research Studies
- Asthma and respiratory diseases
- Nanoparticle-Based Drug Delivery
- Electrospun Nanofibers in Biomedical Applications
- Biosensors and Analytical Detection
- Allergic Rhinitis and Sensitization
- Pickering emulsions and particle stabilization
- Microfluidic and Capillary Electrophoresis Applications
- Cancer Genomics and Diagnostics
- Viral Infections and Vectors
- CRISPR and Genetic Engineering
- Micro and Nano Robotics
- Immune Cell Function and Interaction
- Molecular Biology Techniques and Applications
- Viral Infections and Outbreaks Research
- Vector-Borne Animal Diseases
Purdue University West Lafayette
2008-2021
The University of Texas Medical Branch at Galveston
2000-2019
Galveston College
1997
We have demonstrated the detection of proallergic cytokines in nasal secretions after antigen challenges. Our aim was to determine secretion kinetics chemokines (interleukin [IL]-8, macrophage inflammatory protein-1 alpha [MIP-1 alpha], and RANTES) other (IL-1 beta granulocyte/macrophage colony-stimulating factor [GM-CSF] allergen challenges their inhibition by steroid therapy. Ten allergic patients were given either beclomethasone dipropionate (BDP) or placebo a double-blind, randomized,...
SARS-CoV-2 is the causative agent of COVID-19 pandemic. This in silico study aimed to elucidate therapeutic efficacies against phyco-compounds from seaweed,
An immunofluorescence assay was developed to identify proteins specifically binding oligonucleoside phosphorodithioate (ODN) aptamers from a bead-bound ODN library. Accordingly, NF-kappaB p50 protein incubated with either consensus sequence or combinatorial library and adsorption then assessed using specific primary antibody secondary conjugated Alexa 488 fluorescent dye. This avoids any problems related fluorescently labeling target proteins. The method is straightforward readily applicable...
Abstract Background Most biological samples are cell mixtures. Some basic questions still unanswered about analyzing these heterogeneous using gene expression microarray technology (MAT). How meaningful is a mixture's overall profile (GEP)? Is it necessary to purify the cells of interest before analysis, and how much purity needed? does purification itself distort GEP, well can GEP small subset be recovered? Methods Model mixtures with different ratios were analyzed by both spotted...
Current methods to screen for bacterial contamination involve using costly reagents such as antibodies or PCR time-costly growth in cultures. There is need portable, real-time, multiplex pathogen detection technology that can predict the safety of food. Surface plasmon resonance (SPR) imaging a sensitive, label-free method detect binding an analyte surface by changes refractive index occur upon binding. We have designed hybrid microfluidic biochip perform multiplexed single-celled pathogens...
Current treatments for brain aneurysms are invasive, traumatic, and not suitable in most patients with increased risks. A new alternative method is using scaffold stents to create a local focal attraction force of cells an situ reconstruction the tunica media. For this purpose, nanostructured bioactive coating designed render asymmetric region stent magnetic biomimetic, which utilizes bacterial nanocellulose (BNC) as platform both cell well proliferation. The magnetization BNC realized...
In this study, bacterial nanocellulose (BNC) produced by the bacteria Gluconacetobacter xylinus is synthesized and impregnated in situ with iron oxide nanoparticles (IONP) (Fe3O4) to yield a magnetic (MBNC). The synthesis of MBNC precise specifically designed multi-step process. Briefly, pellicles are formed from preserved G. strain according our experimental requirements size morphology. A solution iron(III) chloride hexahydrate (FeCl3·6H2O) iron(II) tetrahydrate (FeCl2·4H2O) 2:1 molar...
Abstract Surgical clipping and endovascular coiling are well recognized as conventional treatments of Penetrating Brain Injury aneurysms. These clinical approaches show partial success, but often result in thrombus formation the rupture aneurysm near arterial walls. The authors address these challenging brain traumas with a unique combination highly biocompatible biopolymer hydrogel rendered magnetic flexible resilient membrane coating integrated to scaffold stent platform at neck orifice,...
In this study, bacterial nanocellulose (BNC) produced by the bacteria Gluconacetobacter xylinus is synthesized and impregnated in situ with iron oxide nanoparticles (IONP) (Fe3O4) to yield a magnetic (MBNC). The synthesis of MBNC precise specifically designed multi-step process. Briefly, pellicles are formed from preserved G. strain according our experimental requirements size morphology. A solution iron(III) chloride hexahydrate (FeCl3·6H2O) iron(II) tetrahydrate (FeCl2·4H2O) 2:1 molar...
Due to a number of recent technological advances, hand-held flow cytometer can be achieved by use semiconductor illuminators, optical sensors (all battery powered) and sensitive cell markers such as immuno-quantum dot (Qdot) labels. The specific application described is handheld blood analyzer that quickly process drop whole, unfractionated human peripheral real-time, on-chip magnetic separation white cells (WBCs) red (RBCs) further fluorescence analysis Qdot labeled WBC subsets. Various...
Nanomedicine involves cell-by-cell regenerative medicine, either repairing cells one at a time or triggering apoptotic pathways in that are not repairable. Multilayered nanoparticle systems being constructed for the targeted delivery of gene therapy to single cells. Cleavable shells containing targeting, biosensing, and therapeutic molecules direct nanoparticles desired intracellular targets. Therapeutic sequences controlled by biosensor-activated control switches provide proper amount on...
Abstract Background: Scanning cytometry now has many of the features (and power) multiparameter flow while keeping its own advantages as an imaging technology. Modern instruments combine capabilities scanning with ability to manipulate cells. A new technology, called LEAP™ (laser‐enabled analysis and processing), offers a unique combination in cell purification selective macromolecule delivery (optoinjection). Methods: LEAP‐mediated optoinjection effects were assessed model experiments using...
Since current cancer therapies do not work effectively for all patients, there is a pressing need alternate, affordable, yet effective treatments inoperable and chemo-resistant tumors. Presented in this paper are our investigations on the application of short duration (milli micro seconds) electrical voltage pulses treatment breast carcinoma. For purpose, electroporation, pulse power technology, was evaluated its effect upon viability proliferation human cells using number voltages...