A5085011020- Microfluidic and Bio-sensing Technologies
- Advanced biosensing and bioanalysis techniques
- Nanoparticle-Based Drug Delivery
- Single-cell and spatial transcriptomics
- Nanoplatforms for cancer theranostics
- Gene expression and cancer classification
- Microfluidic and Capillary Electrophoresis Applications
- Biosensors and Analytical Detection
- Cancer Genomics and Diagnostics
- RNA Interference and Gene Delivery
- Advanced Biosensing Techniques and Applications
- Electrowetting and Microfluidic Technologies
- Cell Image Analysis Techniques
- Monoclonal and Polyclonal Antibodies Research
- 3D Printing in Biomedical Research
- Molecular Biology Techniques and Applications
- Microbial Inactivation Methods
- Advanced Fluorescence Microscopy Techniques
- Cancer Cells and Metastasis
- Neonatal Respiratory Health Research
- Retinal Development and Disorders
- Nanoparticles: synthesis and applications
- Prenatal Screening and Diagnostics
- Advanced Nanomaterials in Catalysis
- CRISPR and Genetic Engineering
Purdue University West Lafayette
2012-2023
University of Washington
2023
In-Q-Tel
2013
Center for Cancer Research
2011-2013
Korea University
2010
Korea Institute of Science and Technology
2010
Seoul National University
2010
Edinburgh Royal Infirmary
2009
Westmead Hospital
1991-2009
The University of Texas Medical Branch at Galveston
1997-2006
Human immunodeficiency virus (HIV) infects cells after binding of the viral envelope glycoprotein gp120 to cell surface recognition marker CD4. is noncovalently associated with HIV transmembrane gp41, and this complex believed responsible for initial stages infection cytopathic events in infected cells. Soluble constructs CD4 that contain site inhibit vitro. This occur by competitive inhibition cellular Here we suggest an alternative mechanism soluble proteins. We demonstrate biochemically...
Conjugated polymer nanoparticles, produced by in situ colloidal Knoevenagel polymerization, show advantageous properties (bright emission, colloidal/chemical stability and mesoscopic size range) that allow the successful vivo application to real-time sentinel lymph node mapping a mouse model.
Abstract Differences in doublet analysis have the potential to alter DNA cell‐cycle measurements. The techniques for determination are often used interchangeably without regard complexity cell shapes and sizes of biological specimens. G 0/1 doublets were identified quantitated using fluorescence height versus area width pulse measurements, by enumerating proportion 2 + M cells that lack cyclin B1 immunoreactivity, modeled histograms software algorithms. These tested on propidium...
The current study examines the trajectories of student perceived gains as a result time spent in an undergraduate research experience (URE). Data for come from survey administered at three points over 1-yr period: before participation program, end Summer segment research, and year. Repeated-measures analysis variance was used to examine effect on skills, confidence, understanding processes. results suggest that students experienced different gains/benefits developmentally stages their UREs....
We present a disease-on-a-chip model in which cancer grows within phenotypically normal breast luminal epithelium on semicircular acrylic support mimicking portions of mammary ducts. The cells from tumor nodules developing these hemichannels are morphologically distinct their counterparts cultured flat surfaces. Moreover, cocultured with the display different anticancer drug sensitivity compared to surface and monocultures nodules. mimicry development epithelial environment ducts provides...
Abstract The wound healing assay is a commonly used technique to measure cell motility and migration. Traditional methods of performing the suffer from low throughput lack quantitative data analysis. We have developed new method perform high‐throughput that produces using LEAP™ instrument. instrument create reproducible wounds in each well 96‐well plate by laser ablation. then records bright field images at several time points. A custom texture segmentation algorithm determine area point....
Imaging techniques including computed tomography, magnetic resonance imaging, and positron emission tomography (PET) offer many potential benefits to diagnosis treatment of cancers. Each method has its own strong weak points. Therefore, multimodal imaging have been highlighted as an alternative for overcoming the limitations each respective method. In this study, we fabricated PET/optical activatable probe based on glycol chitosan nanoparticles (CNPs) imaging. To prepare dual probes CNPs,...
Obesity results in abnormally high levels of triglyceride (TG) storage tissues such as liver, heart and muscle, which disrupts their normal functions. Recently, we found that lean mice challenged with dietary fat store TGs cytoplasmic lipid droplets the absorptive cells intestine, enterocytes, this increases then decreases over time after an acute challenge. The goal study was to investigate effects obesity on intestinal TG metabolism. More specifically asked whether secretion from intestine...
Iron oxide nanoparticles (IOs) are intrinsically theranostic agents that could be used for magnetic resonance imaging (MRI) and local hyperthermia or tissue thermal ablation. Yet, effective high MR contrast have not been demonstrated within the same nanoparticle configuration. Here, nanoconstructs obtained by confining multiple, ∼ 20 nm nanocubes (NCs) a deoxy-chitosan core. The resulting nanoconstructs-magnetic nanoflakes (MNFs)-exhibit hydrodynamic diameter of 156 ± 3.6 nm, with...
Abstract: While current imaging modalities, such as magnetic resonance (MRI), computed tomography, and positron emission play an important role in detecting tumors the body, no single-modality possesses all functions needed for a complete diagnostic imaging, spatial resolution, signal sensitivity, tissue penetration depth. For this reason, multimodal strategies have become promising tools advanced biomedical research cancer diagnostics therapeutics. In designing nanoparticles,...
Abstract Methods for analyzing very small subpopulations of cells (“rare‐event analysis methods”) are interest in many areas biology. In this paper we demonstrate the ability to detect one Rh‐positive erythrocyte per 100,000 Rh‐negative erythrocytes. This is level needed human fetal red blood maternal circulation case fetal‐maternal Rh‐incompatibility. Erythrocytes stained with fluorescent immunobeads more than 100 times as bright same by indirect immunofluorescence (or almost 40...
In laser flow cytometry, an increasingly popular technique of analytical cytology, quantitative measurements interest include cell and nuclear diameters. Electronic circuitry for a new sizing has been developed which measured the time that signal pulses from either fluorescence or light scatter sensors exceed preset constant fraction peak amplitude (pulse width) it takes to rise between fractions on rising side pulse rise-time). These width rise-time were related diameters used in...
Journal Article Breast on-a-chip: mimicry of the channeling system breast for development theranostics Get access Meggie M.G. Grafton, Grafton Weldon School Biomedical Engineering, Purdue University, West Lafayette, IN 47907-2026, USA Search other works by this author on: Oxford Academic Google Scholar Lei Wang, Wang Department Basic Medical Sciences, Pierre-Alexandre Vidi, Vidi James Leary, Leary USAPurdue Center Cancer Research, USAWeldon USABirck Nanotechnology Center, Discovery Park,...
An immunofluorescence assay was developed to identify proteins specifically binding oligonucleoside phosphorodithioate (ODN) aptamers from a bead-bound ODN library. Accordingly, NF-kappaB p50 protein incubated with either consensus sequence or combinatorial library and adsorption then assessed using specific primary antibody secondary conjugated Alexa 488 fluorescent dye. This avoids any problems related fluorescently labeling target proteins. The method is straightforward readily applicable...