A5056656950- Neurological diseases and metabolism
- Protein Kinase Regulation and GTPase Signaling
- PI3K/AKT/mTOR signaling in cancer
- Porphyrin Metabolism and Disorders
- Ubiquitin and proteasome pathways
- Monoclonal and Polyclonal Antibodies Research
- Cellular transport and secretion
- Metabolism and Genetic Disorders
- Glycosylation and Glycoproteins Research
- RNA modifications and cancer
- Cell Adhesion Molecules Research
- RNA regulation and disease
- Immunotherapy and Immune Responses
- RNA Research and Splicing
- Erythrocyte Function and Pathophysiology
- Cancer-related gene regulation
- Viral Infectious Diseases and Gene Expression in Insects
- Prion Diseases and Protein Misfolding
- Melanoma and MAPK Pathways
- Polyamine Metabolism and Applications
- Chronic Lymphocytic Leukemia Research
- Fibroblast Growth Factor Research
- Peptidase Inhibition and Analysis
- Microtubule and mitosis dynamics
- Mechanisms of cancer metastasis
Institute of Structural and Molecular Biology
2015-2025
University College London
2015-2025
Institute of Molecular Biology and Genetics
2010-2025
National Academy of Sciences of Ukraine
2009-2025
Duke University
2024
Oregon Health & Science University
2024
MRC Unit for Lifelong Health and Ageing
2022-2023
The Royal Free Hospital
2002-2015
Angelina College
2010
Institute of Cancer Research
2007
Insulin-like growth factors elicit many responses through activation of phosphoinositide 3-OH kinase (PI3K). The tuberous sclerosis complex (TSC1-2) suppresses cell by negatively regulating a protein kinase, p70S6K (S6K1), which generally requires PI3K signals for its activation. Here, we show that TSC1-2 is required insulin signaling to PI3K. maintains restraining the activity S6K, when activated inactivates receptor substrate (IRS) function, via repression IRS-1 gene expression and direct...
Evidence is growing for both humoral and cellular immune recognition of human tumor antigens. Antibodies with specificity antigens initially recognized by cytotoxic T lymphocytes (CTLs), e.g., MAGE tyrosinase, have been detected in melanoma patient sera, CTLs NY-ESO-1, a cancer-testis (CT) antigen identified autologous antibody, recently identified. To establish screening system the response to autoimmunogenic antigens, an enzyme-linked immunosorbent assay (ELISA) was developed using...
Activation of the phospholipase D (PLD) pathway is a widespread response when cells are activated by agonists that bind receptors on cell surface. A 16-kD cytosolic component can reconstitute guanosine triphosphate (GTP)-mediated activation in HL60 depleted their cytosol permeabilization. This factor was purified and identified as two small GTP-binding proteins, ARF1 ARF3. Recombinant substituted for ARF proteins reconstitution assay. These results indicate downstream effector The...
Neurodegeneration with brain iron accumulation (NBIA) comprises a clinically and genetically heterogeneous group of disorders progressive extrapyramidal signs neurological deterioration, characterized by in the basal ganglia. Exome sequencing revealed presence recessive missense mutations COASY, encoding coenzyme A (CoA) synthase one NBIA-affected subject. second unrelated individual carrying COASY was identified Sanger sequence analysis. CoA is bifunctional enzyme catalyzing final steps...
Neutrophils possess a multicomponent NADPH oxidase system capable of producing large quantities superoxide in process known as the respiratory burst (1). Upon stimulation phagocytic cell, two cytosolic components oxidase, p67(phox) and p47(phax), associate with membrane-bound flavocytochrome b small GTP-binding protein to form functional enzyme complex. Each Phox proteins contains src homology 3 (SH3) domains, which are unknown function but potential mediators protein-protein interactions...
The interaction between SH2 domains and phosphotyrosine-containing sequences was examined by real-time measurements of kinetic parameters. the p85 subunit phosphatidylinositol 3-kinase as well other signaling molecules were expressed in bacteria glutathione S-transferase fusion proteins. Phosphotyrosine-containing peptides, corresponding to two autophosphorylation sites on human platelet-derived growth factor beta-receptor that are responsible for binding, synthesized used capturing...
A novel ribosomal S6 kinase, termed p70 kinase β (p70β), which has a highly conserved amino acid sequence compared with that of p70/p85 (p70α) within the catalytic, extension, and autoinhibitory pseudosubstrate domains, was identified. However, p70β differs from p70α in noncatalytic amino-terminal region carboxyl-terminal tail, contains proline-rich region. The majority regulatory phosphorylation sites identified are p70β. Two isoforms p70β, referred to as β1 (495 acids) β2 (482 acids),...