A5006872975- Neurotransmitter Receptor Influence on Behavior
- Neuroscience and Neuropharmacology Research
- Genetics and Neurodevelopmental Disorders
- Protein Kinase Regulation and GTPase Signaling
- Receptor Mechanisms and Signaling
- Parkinson's Disease Mechanisms and Treatments
- Genomics and Rare Diseases
- Neurogenesis and neuroplasticity mechanisms
- Olfactory and Sensory Function Studies
- Advanced Chemical Sensor Technologies
- Autism Spectrum Disorder Research
- COVID-19 Clinical Research Studies
- Mitochondrial Function and Pathology
- SARS-CoV-2 and COVID-19 Research
- PI3K/AKT/mTOR signaling in cancer
- Ion channel regulation and function
- Porphyrin Metabolism and Disorders
- SARS-CoV-2 detection and testing
- Hedgehog Signaling Pathway Studies
- Biochemical Analysis and Sensing Techniques
- Neurological diseases and metabolism
- Ubiquitin and proteasome pathways
- Melanoma and MAPK Pathways
- Diabetes Treatment and Management
- Amino Acid Enzymes and Metabolism
Institut de Psychiatrie et Neurosciences de Paris
2020-2024
Université Paris Cité
2020-2024
Inserm
2020-2024
Centre Hospitalier Sainte-Anne
2024
Danube Private University
2020-2024
FHU Neurovasc
2020-2022
City University of New York
2017-2021
City College of New York
2015-2021
Università Cattolica del Sacro Cuore
2021
CUNY School of Law
2015-2020
Insulin-like growth factors elicit many responses through activation of phosphoinositide 3-OH kinase (PI3K). The tuberous sclerosis complex (TSC1-2) suppresses cell by negatively regulating a protein kinase, p70S6K (S6K1), which generally requires PI3K signals for its activation. Here, we show that TSC1-2 is required insulin signaling to PI3K. maintains restraining the activity S6K, when activated inactivates receptor substrate (IRS) function, via repression IRS-1 gene expression and direct...
Background. In a combined animal and human study, we have previously found that 5-day treatment enhances cortical plasticity also facilitates brain-derived neurotrophic factor (BDNF)-tyrosine receptor kinase B (TrkB) signaling increases activated TrkB N-methyl-d-aspartate (NMDAR) association in both the cortex peripheral lymphocytes. Patients with Parkinson’s disease (PD), general, show decreased plasticity, as demonstrated by electrophysiological behavioral studies. Here, test hypothesis an...
CoA synthase mediates the last two steps in sequence of enzymatic reactions, leading to biosynthesis. We have recently identified cDNA for and demonstrated that it encodes a bifunctional enzyme possessing 4'-phosphopantetheine adenylyltransferase dephospho-CoA kinase activities. Molecular cloning provided us with necessary tools study subcellular localization regulation this enzyme. Transient expression studies confocal microscopy allowed demonstrate full-length is associated mitochondria,...
The ribosomal protein S6 kinase (S6K) belongs to the AGC family of Ser/Thr kinases and is known be involved in regulation synthesis G(1)/S transition cell cycle. There are two forms S6K, termed S6Kalpha S6Kbeta, which have cytoplasmic nuclear splice variants. Nucleocytoplasmic shuttling has been recently proposed for S6Kalpha, based on use export inhibitor, leptomycin B. However, molecular mechanisms regulating subcellular localization S6Ks response mitogenic stimuli remain elucidated. Here...
Dopamine neurotransmission controls motor and perseverative behavior, is mediated by protein phosphorylation, may be perturbed in disorders of attention hyperactivity. To assess the role casein kinase I (CK1) regulation dopamine signaling, we generated a genetically modified mouse line that overexpresses CK1δ (CK1δ OE) specifically forebrain. Overexpression was confirmed both at mRNA levels. Under basal conditions, OE mice exhibited horizontal vertical hyperactivity, reduced anxiety, nesting...
Parkinson's disease is caused by a selective vulnerability and cell loss of dopaminergic neurons the Substantia Nigra pars compacta and, consequently, striatal dopamine depletion. In therapy, counteracted administration L-DOPA, which initially effective in ameliorating motor symptoms, but over time leads to burdening side effect uncontrollable jerky movements, termed L-DOPA-induced dyskinesia. To date, no efficient treatment for dyskinesia exists. The serotonergic systems are intrinsically...
Coenzyme A functions as a carrier of acetyl and acyl groups in living cells is essential for numerous biosynthetic, energy-yielding, degradative metabolic pathways. There are five enzymatic steps CoA biosynthesis. To date, molecular cloning enzymes involved the biosynthetic pathway mammals has been only reported pantothenate kinase. In this study, we present cDNA functional characterization synthase. It an open reading frame 563 aa encodes protein ∼60 kDa. Sequence alignments suggested that...
Significance G protein-coupled receptors (GPCRs) regulate the activity of virtually all cell types including pancreatic β-cells. β-Cell M 3 muscarinic (M3Rs) play an essential role in maintaining proper whole-body glucose homeostasis. Activity M3R, like that other GPCRs, is modulated by phosphorylation various kinases, GRKs and casein kinase 2 (CK2). The potential physiological relevance M3R (or GPCRs general) CK2 remains unknown. We here show CK2-dependent β-cell M3Rs significantly impairs...
Abstract The dopamine D5 receptor (D5R) is a Gα s -coupled belonging to the D1-like family. Together with D2 it highly expressed in striatal cholinergic interneurons and therefore poised be positive regulator of activity response L-DOPA dopamine-depleted parkinsonian brain. Tonically active become dysregulated during chronic administration participate expression induced dyskinesia. molecular mechanisms involved this process have not been elucidated, however correlation between dyskinesia...
We present evidence, using biochemical and cellular approaches, that the kinase, CK2, negatively controls signaling via Gα s (or olf ) coupled to dopamine D1 adenosine A2A receptors. Pharmacological inhibition of CK2 or knockdown by RNAi lead elevated cAMP levels in receptor-activated neuroblastoma cells. Phosphorylation protein kinase A substrates were increased presence inhibitors mouse striatal slices. The effect receptor agonists on phosphorylation sites was potentiated upon inhibition....
Abstract L-Dopa induced dyskinesia (LID) is a debilitating side effect of dopamine replacement therapy for Parkinson’s Disease. The mechanistic underpinnings LID remain obscure. Here we report that diminished sonic hedgehog (Shh) signaling in the basal ganglia caused by degeneration midbrain neurons facilitates formation and expression LID. We find pharmacological activation Smoothened, downstream effector Shh, attenuates neurotoxic 6-OHDA- genetic aphakia mouse models Employing conditional...
Ribosomal protein S6 kinase (S6K) is activated by an array of mitogenic stimuli and a key player in the regulation cell growth. The activation process involves complex sequential series multiple Ser/Thr phosphorylations mainly mediated via phosphatidylinositol 3‐kinase (PI3K)‐3‐phosphoinositide‐dependent kinase‐1 (PDK1) mTor‐dependent pathways. Upstream regulators S6K, such as PDK1 B (PKB/Akt), are recruited to membrane their pleckstrin homology (PH) or protein–protein interaction domains....
We have previously shown that casein kinase 2 (CK2) negatively regulates dopamine D1 and adenosine A 2A receptor signaling in the striatum. Ablation of CK2 receptor-positive striatal neurons caused enhanced locomotion exploration at baseline, whereas ablation D2 increased after treatment with antagonist, caffeine. Because both, receptors, play major roles cellular responses to l -DOPA striatum, these findings prompted us examine impact on effects unilateral 6-OHDA lesioned mouse model...
The Lower Austrian Wachau region was an early COVID-19 hotspot of infection. As previously reported, in June 2020, after the first peak infections, we determined that 8.5% and 9.0% participants Weißenkirchen surrounding communities were positive for immunoglobulin G (IgG) A (IgA) antibodies against receptor-binding domain spike protein SARS-CoV-2, respectively. Here, present novel data obtained eight months later (February 2021) from Weißenkirchen, second infection, with 25.0% (138/552)...