A5068324301- Neuroscience and Neuropharmacology Research
- Receptor Mechanisms and Signaling
- Neurotransmitter Receptor Influence on Behavior
- Genetics and Neurodevelopmental Disorders
- ATP Synthase and ATPases Research
- Biosimilars and Bioanalytical Methods
- Parkinson's Disease Mechanisms and Treatments
- Enzyme Structure and Function
- Microtubule and mitosis dynamics
- Ion channel regulation and function
- Optical measurement and interference techniques
- Spectroscopy Techniques in Biomedical and Chemical Research
- Advanced NMR Techniques and Applications
- Scientific Computing and Data Management
- Innovative Microfluidic and Catalytic Techniques Innovation
- Nitric Oxide and Endothelin Effects
- Infrared Thermography in Medicine
- Autophagy in Disease and Therapy
- CRISPR and Genetic Engineering
- PI3K/AKT/mTOR signaling in cancer
- Cellular transport and secretion
Johns Hopkins University
2019-2023
Johns Hopkins Medicine
2020-2023
CUNY School of Law
2017-2020
Western New England University
2010
New England College
2010
We have recently described an A350V mutation in IQSEC2 associated with intellectual disability, autism and epilepsy. sought to understand the molecular pathophysiology of this goal developing targets for drug intervention. demonstrate here that results interference binding apocalmodulin IQ domain IQSEC2. further constitutive activation guanine nucleotide exchange factor (GEF) activity resulting increased production active form Arf6. In a CRISPR generated mouse model mutation, we surface...
Abstract The dopamine D5 receptor (D5R) is a Gα s -coupled belonging to the D1-like family. Together with D2 it highly expressed in striatal cholinergic interneurons and therefore poised be positive regulator of activity response L-DOPA dopamine-depleted parkinsonian brain. Tonically active become dysregulated during chronic administration participate expression induced dyskinesia. molecular mechanisms involved this process have not been elucidated, however correlation between dyskinesia...
Innovation in pharmaceutical therapeutics is critical for the treatment of serious diseases with unmet medical need. To accelerate approval these innovative treatments, regulatory agencies throughout world are increasingly adopting use expedited pathways and collaborative reviews. These primarily driven by promising clinical results but become challenging Chemistry, Manufacturing, Controls (CMC) information submissions. Condensed shifting timelines present constraints that require new...
We have previously shown that casein kinase 2 (CK2) negatively regulates dopamine D1 and adenosine A 2A receptor signaling in the striatum. Ablation of CK2 receptor-positive striatal neurons caused enhanced locomotion exploration at baseline, whereas ablation D2 increased after treatment with antagonist, caffeine. Because both, receptors, play major roles cellular responses to l -DOPA striatum, these findings prompted us examine impact on effects unilateral 6-OHDA lesioned mouse model...
The regulation of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) trafficking affects multiple brain functions, such as learning and memory. We have previously shown that Thorase plays an important role in the internalization AMPARs from synaptic membrane. Here, we show N-methyl-d-aspartate (NMDAR) activation leads to increased S-nitrosylation N-ethylmaleimide-sensitive factor (NSF). stabilizes Thorase-AMPAR complexes enhances AMPAR interaction with protein-interacting...
The mechanistic target of rapamycin (mTOR) signals through the mTOR complex 1 (mTORC1) and 2 to maintain cellular organismal homeostasis. Failure finely tune activity results in metabolic dysregulation disease. While there is substantial understanding molecular events leading mTORC1 activation at lysosome, remarkably little known about what terminates signaling. Here, we show that AAA + ATPase Thorase directly binds mTOR, thereby orchestrating disassembly inactivation mTORC1. disrupts...
Abstract The AAA+ ( A TPases a ssociated with variety of cellular ctivities) ATPase, Thorase, also known as ATAD1, plays multiple roles in synaptic plasticity, mitochondrial quality control and mTOR signaling through disassembling protein complexes like AMPAR mTORC1 an ATP-dependent manner. Oligomerization Thorase is crucial for its disassembly remodeling functions. We show that wild-type forms long helical filaments vitro , dependent on ATP binding but not hydrolysis. report the Cryogenic...
In this study a whole field, non-contact optical method, Stereo Digital Image Correlation (SDIC), was used to determine the strain distribution and mechanical properties of fresh bone in Phosphate Buffered Saline (PBS) solution. Knowing whole-surface is useful for understanding effects normal physiological loading, disease, drugs aging. addition, knowing will aid design new biomaterials. Although there currently are methods measuring bone, these have some limitations. Many miss areas...
An amendment to this paper has been published and can be accessed via a link at the top of paper.