A5088793592- Metabolomics and Mass Spectrometry Studies
- Mass Spectrometry Techniques and Applications
- Analytical Chemistry and Chromatography
- Metabolism, Diabetes, and Cancer
- Research on Leishmaniasis Studies
- Liver Disease Diagnosis and Treatment
- Trypanosoma species research and implications
- Parasites and Host Interactions
- Physiological and biochemical adaptations
- Alcohol Consumption and Health Effects
- Stress Responses and Cortisol
- Gut microbiota and health
- Liver physiology and pathology
- Growth Hormone and Insulin-like Growth Factors
- Microbial Natural Products and Biosynthesis
- Enzyme function and inhibition
- Genomics and Phylogenetic Studies
- Lipid metabolism and biosynthesis
- Advanced Chemical Sensor Technologies
- Bone health and treatments
- Probiotics and Fermented Foods
- Diet and metabolism studies
- Advanced Proteomics Techniques and Applications
- Microfluidic and Capillary Electrophoresis Applications
- Fermentation and Sensory Analysis
Universidad San Pablo CEU
2019-2024
Instituto de Salud Carlos III
2023
Background: Leishmaniases are neglected tropical diseases that caused by Leishmania, being endemic worldwide. L-arginine is an essential amino acid required for polyamines production on mammal cells. During Leishmania infection of macrophages, used host and parasite arginase to produce polyamines, leading survival; or, nitric oxide synthase 2 killing. Here, we determined the metabolomic profile BALB/c macrophages were infected with L. amazonensis wild type or knockout, correlating regulation...
The alteration of modified amino acid (MAA) profiles in biological samples is related to important cellular, physiological, and pathological processes. To achieve the interpretation their biochemical relevance, it critical define whole chemical spectrum using metabolomic research works. We present a detailed in-source fragmentation (ISF) study based on mechanisms major reactions observed diagnostic ions (DIs) generated positive electrospray ionization for 57 standard compounds capillary...
Metabolites are the final products of metabolism and are, therefore, directly related to phenotype. They constitute metabolome an organism. The wide diversity physicochemical properties metabolites in terms molecular weight, concentration, polarity, volatility, solubility, pKa, charge makes their analysis a remarkable challenge. With over 220,000 recorded HMDB database, there is no single analytical technique capable analyzing all them. Therefore, multiple platforms required obtain...
Leishmania survival inside macrophages depends on factors that lead to the immune response evasion during infection. In this context, metabolic scenario of host cell–parasite relationship can be crucial understanding how parasite survive cells due host’s pathways reprogramming. work, we aimed analyze networks bone marrow-derived from C57BL/6 mice infected with amazonensis wild type (La-WT) or arginase knocked out (La-arg−), using untargeted Capillary Electrophoresis-Mass Spectrometry (CE-MS)...
The liver is the only solid organ capable of regenerating itself to regain 100% its mass and function after injury and/or partial hepatectomy (PH). This exceptional property represents a therapeutic opportunity for severe disease patients. However, regeneration (LR) might fail due poorly understood causes. Here, we have investigated regulation proteome phosphoproteome at short time PH (9 h), depict detailed mechanistic background early LR phase. Furthermore, analyzed dynamic changes serum...
Since l-argininosuccinic acid (ASA) is the characteristic biomarker for diagnosis of certain diseases, its reliable detection in complex biological samples necessary to obtain a complete evaluation with greater specificity and accuracy. ASA can undergo intramolecular cyclization, yielding an equilibrium resulting cyclic forms, which predominate under different analytical conditions. In this work, appearance transformation forms have been studied strategy targeted screening analysis using...
The Aspergillus Metabolome Database is a free online resource to perform metabolite annotation in mass spectrometry studies devoted the genus Aspergillus. database was created by retrieving and curating information on 2811 compounds present 601 different species subspecies of A total 1514 scientific journals where these metabolites are mentioned were added as meta-information linked their respective database. web service query based m/z (mass/charge ratio) searches CEU Mass Mediator; queries...
The composition of wine is determined by a complex interaction between environmental factors, genetic factors (i.e., grape varieties), and winemaking practices (including technology storage). Metabolomics using NMR spectroscopy, GC-MS, and/or LC-MS has shown to be useful approach for assessing the origin, authenticity, quality various wines. Nonetheless, use additional analytical techniques with complementary separation mechanisms may aid in deeper understanding wine's metabolic processes....
Abstract In contemporary biomedical research, the zebrafish ( Danio rerio ) is increasingly considered a model system, as embryos and larvae can (potentially) fill gap between cultured cells mammalian animal models, because they be obtained in large numbers, are small easily manipulated genetically. Given that capillary electrophoresis–mass spectrometry (CE–MS) useful analytical separation technique for analysis of polar ionogenic metabolites biomass‐limited samples, aim this study was to...