An allotypic form of the low affinity IgG Fc receptor gamma RIIa (CD32), termed responder (LR) because its weak reactivity with mouse (m) IgG1, interacts efficiently human (h) IgG2. RIIaLR is first known FcR that binds this subclass. In study, we analyzed role in binding stable hIgG-subclass dimers, and induction T cell mitogenesis using chimeric anti-CD3 mAb. We demonstrate functional polymorphism to hIgG2 expressed on majority R-bearing peripheral blood cells: monocytes, neutrophils,...

Natural antibodies (NAb) are defined as present in individuals without known antigenic challenge. Levels of NAb binding keyhole limpet hemocyanin (KLH) chickens were earlier shown to be heritable, and associated with survival. Selective breeding may thus provide a strategy improve natural disease resistance. We phenotyped 3,689 white purebred laying for KLH different isotypes around 16 weeks age. Heritabilities 0.12 the titers total (IgT), 0.14 IgM, 0.10 IgA, 0.07 IgG estimated. also...

Keyhole limpet hemocyanin (KLH)-binding natural antibody (NAb) titers in chickens are heritable, and higher levels have previously been associated with a survival. This suggests that selective breeding for NAb might increase survival by means of improved general disease resistance. Chickens were divergently selected bred total binding KLH at 16 weeks age six generations, resulting High selection line Low line. To test differences resistance, challenged avian pathogenic Escherichia coli...

This study addressed the impact of early and later life environmental enrichment, their combination, on specific antibody responses peripheral blood leukocyte subpopulations in pigs. Pigs were kept either barren (B1) or enriched (E1) housing from birth, half pigs switched to day 47, resulting four treatment combinations: B1B2, B1E2, E1B2, E1E2). immunized with keyhole limpet hemocyanin-conjugated trinitrophenyl (KLH-TNP) 74 109 induce primary secondary responses. Blood samples taken weekly...

Natural antibodies (NAb) are an important component of the first line immune defense. Selective breeding for enhanced NAb levels in chickens may improve general disease resistance. It is unknown what consequences selection will be on productive performance laying hens. In this paper we describe genetic relations between titers binding keyhole limpet hemocyanin at 19 wk age and production traits a white purebred leghorn chicken observed several time periods. A linear animal model was used to...

Natural antibodies (NAb) are antigen binding present in individuals without a previous exposure to this antigen. Keyhole limpet hemocyanin (KLH)-binding NAb levels were previously associated with survival chickens. This suggests that selective breeding for KLH-binding may increase by means of improved general disease resistance. Genome-wide association studies (GWAS) performed identify genes underlying genetic variation levels. The studied population consisted 1,628 adolescent layer chickens...

A sufficient IgG content in the colostrum is essential for newborn calf, as it provides passive immunity which substantially affects probability of survival during rearing. Failure transfer (FPT) occurs when a calf does not absorb enough antibodies from and defined by an concentration serum lower than 10 g/L. Apart delayed access to colostrum, FPT can be due low production mother or poor absorption calf. The aim this study was estimate genetic background antibody levels indicator traits...

Recently, we have reported trained innate immunity in laying chicken monocytes. In the present study, further investigated of monocytes layers and broilers. Monocytes both breeds isolated from blood were vitro with β-glucan, rec-chicken IL-4 or a combination both, restimulated lipopolysaccharide (LPS), after which inflammation metabolism-related responses measured. Training broiler hen resulted increased mRNA levels IL-1β, iNOS HIF-1α, but enhanced surface expression CD40 NO production was...

NAb are defined as antigen binding antibodies present without a known previous exposure to this antigen. suggested enhance specific antibody (SpAb) responses, but consequences of different levels on immunization largely unknown. Layer chickens were divergently selected and bred for keyhole limpet hemocyanin (KLH)-binding titers, resulting in High line Low line. In study, we investigated: (1) the relation with SpAb titers; (2) effect titers. The 50 highest females lowest generation 2...

Intestinal pathology early in life may affect immune development and therefore responses later life. Dextran sulfate sodium (DSS) induces colitis rodents is a widely used model for inflammatory bowel diseases. The present study investigated DSS as intestinal its consequences on pathology, ileal cytokine, immunoglobulin mRNA expression levels well the antibody response towards an immunological challenge chickens. Broiler layer chicks received 2.5% drinking water during d 11 through 18 post...

Gut microbiotas play a pivotal role in host physiology and behaviour, may affect life-history traits such as seasonal variation phenotypic state. Generally, gut microbiota is attributed to diet variation. However, temperature day length also drive We investigated summer-winter differences the bacterial community (GBC) 14 homing pigeons living outdoors under constant by collecting cloacal swabs both seasons during two years. Because effects be mediated metabolism, we determined basal...

The presence and relative levels (titers) of IgM IgG natural antibodies (NAb) binding keyhole limpet hemocyanin (KLH), (auto-) (N(A)Ab) salmon double-stranded DNA (dsDNA), (oxidated-) phosphatidyl (phosphoryl) choline-conjugated bovine serum albumin (PC-BSA), PC-conjugated ovalbumin (PC-OVA), OVA, respectively, were studied in adult hen plasma, egg yolk, albumen, plasma their hatchlings, 8-day-old chick plasma. Birds eggs from 2 lines divergently selected for high or low NAb KLH. This study...

In 2 experiments, interactions between trace mineral (Zn, Mn, Cu, Se) source (organic or inorganic) in the broiler breeder diet and egg translucency (high low) on characteristics embryonic development were investigated. first experiment, eggs from old breeders (55-57 wk) second prime (34-36 used. Egg composition bacterial load eggshell analyzed fresh eggs. During incubation, metabolic heat production of embryos (d 8 (E8) to 19 incubation) tibia ossification (E8.5-E14.5) determined daily. At...

Natural antibodies (NAb) are that present in a healthy individual without requiring previous exposure to an exogenous antigen. Selection for high NAb levels might contribute improved general disease resistance. Our aim was analyse the genetic background of based on divergent selection experiment poultry, and particular effect polymorphism TLR1A gene.The study population consisted base from commercial pure-bred elite white leghorn layer line seven generations birds High Low line. Birds were...

Activation of the maternal immune system may affect innate and adaptive responses in next generation therefore have implications for vaccine efficacy dietary modulation by feed additives. However, transgenerational effects on chickens been investigated to a limited extend. The present study intratracheal (i.t) specific aspecific activation laying hens antibody production generation. In two experiments received intratracheally an stimulus with human serum albumin (HuSA) or lipopolysaccharide...

Abstract Background Colostrum with sufficient IgG content is essential for the newborn calf, as it provides passive immunity which substantially affects probability of survival during rearing. Failure transfer occurs when a calf does not absorb enough antibodies from colostrum indicated by less than 10 g/L in serum. Besides delayed access to colostrum, this can be due low production mother or poor absorption calf. The aim study was estimate genetic background antibody levels and indicator...