Bovine coronavirus (BCoV) is a widely distributed pathogen, causing disease and economic losses in the cattle industry worldwide. Prevention of virus spread impeded by lack basic knowledge concerning viral shedding transmission potential individual animals. The aims study were to investigate duration quantity BCoV feces nasal secretions related clinical signs, presence blood tissues test hypothesis that seropositive calves are not infectious naïve in-contact three weeks after infection. A...

ABSTRACT Two genetically distinct bovine enteric caliciviruses (BECs) have been identified: the norovirus (NLV) Jena and Newbury Agent-2 (NA-2) BECs, which are related to human noroviruses, Nebraska (NB) is sapoviruses lagoviruses but may also represent a new calicivirus genus. The prevalence of these two BEC genotypes in cattle unknown. Although reverse transcription-PCR (RT-PCR) primers for NLV recognize NLV-BECs, genetic relationships between from humans NLV-BECs commonly circulating...

Coronaviruses demonstrate great potential for interspecies transmission, including zoonotic outbreaks. Although bovine coronavirus (BCoV) strains are frequently circulating in cattle farms worldwide, causing both enteric and respiratory disease, little is known about their genomic evolution. We sequenced analysed the full-length spike (S) protein gene of 33 BCoV from dairy feedlot collected during outbreaks that occurred 2002 to 2010 Sweden Denmark. Amino acid identities were >97 % this...

Summary Six calves, aged 24 to 58 days and not previously exposed bovine viral diarrhoea virus (BVDV), were infected with this agent by nose‐to‐nose contact a persistently BVDV viraemic calf. The study was conducted in two trials, using 3 calves each. All 6 showed peak interferon level serum at 4 post infection (dpi), they seroconverted 16–21 dpi. trial 1 had for 2 or between dpi one calf again from 9 11 During the periods of fever, slightly depressed. Those more depressed their oral nasal...

Body condition score and blood profiles have been used to monitor management herd health in dairy cows. The aim of this study was examine BCS extended metabolic profiles, reflecting both energy metabolism liver status around calving high-producing herds with a high incidence abomasal displacement ketosis evaluate if such can be at level pinpoint specific problems.Body five incidences were assessed using linear mixed models (94 cows, 326 examinations). Cows examined sampled every three weeks...

A cross‐sectional study was conducted to evaluate the association between herd‐level characteristics, management routines and farm‐level antibody status of bovine coronavirus (BCV) respiratory syncytial virus (BRSV) in 257 Swedish dairy herds. The possible spatial clustering positive herds compared with negative also investigated. For each herd, a pooled milk sample from five primiparous cows analysed for presence antibodies BCV BRSV. Herd‐level information obtained by questionnaire....

A sufficient IgG content in the colostrum is essential for newborn calf, as it provides passive immunity which substantially affects probability of survival during rearing. Failure transfer (FPT) occurs when a calf does not absorb enough antibodies from and defined by an concentration serum lower than 10 g/L. Apart delayed access to colostrum, FPT can be due low production mother or poor absorption calf. The aim this study was estimate genetic background antibody levels indicator traits...

In order to prevent spread of the endemic pathogens bovine coronavirus (BCoV) and respiratory syncytial virus (BRSV) between herds, knowledge indirect transmission by personnel fomites is fundamental. The aims study were determine duration viral RNA carriage infectivity particles on human nasal mucosa after exposure BCoV BRSV. During two animal infection experiments, swabs collected from (nasal mucosa) their clothes, boots equipment contact with calves shedding either virus. Viral was...

Bovine respiratory syncytial virus (BRSV) and bovine corona (BCV) affects cattle worldwide. Our objective was to evaluate the effects of these infections on general health reproduction parameters measurable herd level explore association between antibody status some characteristics.We collected a pooled milk sample from five primiparous cows 79 Swedish dairy herds in September 2006. The samples were analysed for immunoglobulin G antibodies BCV BRSV with indirect enzyme-linked immunosorbent...

Bulk milk samples from 2236 dairy herds randomly selected throughout Sweden in proportion to region and herd size were analysed for antibodies bovine coronavirus (BCV) an ELISA. The results expressed as optical density (OD) values OD > 0.04 was considered positive. Eighty-nine per cent of the antibody-positive 52 had high levels BCV (an 0.70). There significantly higher (P < 0.001) fewer antibody-negative larger than smaller herds. also southern northern 0.001 P 0.001, respectively). These...

Summary Injections of glucagon in dairy cows resulted a rapid and marked increase serum insulin also plasma glucose. Glucagon injection appears to be simple useful method for testing secretion glucose release. There was somewhat reduced response 2 weeks after calving normal cows. Cows given high intensity feeding before showed higher levels the test 3 days post partum signs sensitivity action. with abomasal displacement impaired tolerance heterogeneity responses indicating resistance Type...

This study evaluated the effects of supplemental low- and high-purity glycerine on silage intake, milk yield composition, plasma metabolites body condition score (BCS) in dairy cows. A total 42 cows Swedish Red Breed, housed individual tie stalls, were fed 0.25 kg or top concentrate, twice daily, during first 4 weeks lactation. One-third acted as controls, receiving no glycerine. Silage was for ad libitum intake concentrate at restricted level about 6 kg/day primiparous 7 multiparous Feed...

Altogether 218 sheep sera from 40 flocks in different parts of Sweden were screened for antibodies to bovine Coronavirus (BCV). Nineteen per cent the positive and there was a significantly higher frequency (p<0.05) at least one sample with more than 100 adult smaller flocks. There also (p<0.001) samples older 4 years younger ones. Only weak relationship between BCV positivity (2 or samples, p<0.05) cattle contact demonstrated this study. Possible transmission routes other factors that could...

This study evaluated whether differences in milk fever incidence among Swedish dairy herds could be explained partly by mineral feeding during the last weeks of gestation. A case–control was performed on dietary risk factors for a high at herd level using information regarding and management dry cows collected written questionnaire distributed post spring 2008. The conducted from September 2004 to August 2007. Data diets fed cows, with an emphasis amounts minerals (Ca, P, Mg K) 3 before...