A5072199740- Cellular transport and secretion
- Neuroscience and Neuropharmacology Research
- Lipid Membrane Structure and Behavior
- CRISPR and Genetic Engineering
- Neurological disorders and treatments
- Biomedical Text Mining and Ontologies
- Biotin and Related Studies
- International Science and Diplomacy
- Health and Medical Research Impacts
- Neurobiology and Insect Physiology Research
- RNA regulation and disease
- Trypanosoma species research and implications
- Science, Research, and Medicine
- Genomics and Chromatin Dynamics
- Bioinformatics and Genomic Networks
- Endoplasmic Reticulum Stress and Disease
- DNA Repair Mechanisms
- Aquaculture disease management and microbiota
- Neurogenesis and neuroplasticity mechanisms
- Machine Learning in Bioinformatics
- Advanced Fluorescence Microscopy Techniques
- Nerve injury and regeneration
- Parasites and Host Interactions
- Research on Leishmaniasis Studies
- Parkinson's Disease Mechanisms and Treatments
University of Bonn
2022
Tufts University
2016-2019
Amsterdam Neuroscience
2015
Vrije Universiteit Amsterdam
2009-2014
Genomics (United Kingdom)
2014
The Netherlands Cancer Institute
2007
Academic Medical Center
2006
DNA double-strand breaks (DSBs) can efficiently kill cancer cells, but they also produce unwanted chromosome rearrangements when ends from different DSBs are erroneously joined. Movement of DSB-containing chromatin domains might facilitate these DSB interactions and promote the formation rearrangements. Therefore, we analyzed mobility containing DSBs, marked by fluorescently tagged marker 53BP1, in living mammalian cells compared it with undamaged on a time-scale relevant for repair. We...
Palmitoylation, a post-translational modification of cysteine residues with the lipid palmitate, has recently emerged as an important mechanism for regulating protein trafficking and function. With identification 23 DHHC mammalian palmitoyl acyl transferases (PATs), key question was nature substrate-enzyme specificity these PATs. Using acyl-biotin exchange palmitoylation assay, we compared substrate four neuronal PATs, namely DHHC-3, DHHC-8, HIP14L (DHHC-13), HIP14 (DHHC-17). Exogenous...
Synapses are specialized neuronal cell-cell contacts that underlie network communication in the mammalian brain. Across populations and circuits, a diverse set of synapses is utilized, they differ their molecular composition to enable heterogenous connectivity patterns functions. In addition pre- post-synaptic specializations, synaptic cleft now understood be an integral compartment contributes structural functional organization. Aiming map proteome, this study applied peroxidase-mediated...
Munc18-1 is a soluble protein essential for synaptic transmission. To investigate the dynamics of endogenous in neurons, we created mouse model expressing fluorescently tagged from munc18-1 locus. We show using fluorescence recovery after photobleaching hippocampal neurons that majority trafficked through axons and targeted to synapses via lateral diffusion together with syntaxin-1. was strongly expressed at presynaptic terminals, individual showing large variation expression. Axon–synapse...
Base J or β-d-glucosylhydroxymethyluracil is a DNA modification replacing fraction of thymine in the nuclear kinetoplastid parasites and Euglena. located telomeric sequences Trypanosoma brucei other simple repeat sequences. In addition, was found inactive variant surface glycoprotein (VSG) expression sites, but not active site T. brucei, suggesting that could play role transcription silencing brucei. We have now looked at distribution genomes parasites. First, we analyzed immunoprecipitated...
The munc18-1 gene encodes two splice-variants that vary at the C-terminus of protein and are expressed different levels in regions adult mammalian brain. Here, we investigated expression pattern these splice variants within brainstem tested whether they functionally different. Munc18-1a is specific nuclei including LRN, VII SOC, while Munc18-1b relatively low/absent regions. Furthermore, major variant Calyx Held. Synaptic transmission was analyzed autaptic hippocampal KO neurons...
Introduction Alzheimer's disease (AD) and Parkinson's (PD) are neurodegenerative disorders causing cognitive deficits motor difficulties in the elderly. Conventional treatments mainly symptomatic with little ability to halt progression. Gene therapies correct or silence genetic mutations predisposing AD PD currently being developed preclinical studies clinical trials, relying mostly on systemic delivery, which reduces their effectiveness. Imaging-guided stereotaxic procedures used locally...