A5024700545- Neuroscience and Neuropharmacology Research
- Lipid Membrane Structure and Behavior
- Cellular transport and secretion
- Pain Mechanisms and Treatments
- Exercise and Physiological Responses
- Photoreceptor and optogenetics research
- Force Microscopy Techniques and Applications
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Biotin and Related Studies
- Infrared Thermography in Medicine
- Musculoskeletal pain and rehabilitation
- Photochromic and Fluorescence Chemistry
University of Colorado Anschutz Medical Campus
2023-2024
University of Maryland, Baltimore
2018-2022
Duquesne University
2016-2019
The nanoscale distribution of AMPA receptors is under rapid control by adhesion proteins and helps regulate their activation.
Synapses are specialized neuronal cell-cell contacts that underlie network communication in the mammalian brain. Across populations and circuits, a diverse set of synapses is utilized, they differ their molecular composition to enable heterogenous connectivity patterns functions. In addition pre- post-synaptic specializations, synaptic cleft now understood be an integral compartment contributes structural functional organization. Aiming map proteome, this study applied peroxidase-mediated...
Numerous qualitative and quantitative techniques can be used to test sensory nerves pain in both research clinical settings. The current study demonstrates a testing protocol using measure tactile sensation threshold for pressure heat portable easily accessed equipment. These equipment are ideal new laboratories clinics where cost is concern or limiting factor. We demonstrate measurement the following: cutaneous mechanical sensitivity on arms legs (von-Frey filaments), radiant contact (with...
Postsynaptic neurotransmitter receptors and their associated scaffolding proteins assemble into discrete, nanometer-scale subsynaptic domains (SSDs) within the postsynaptic membrane at both excitatory inhibitory synapses. Intriguingly, receptor SSDs are mirrored by closely apposed presynaptic active zones. These trans-synaptic molecular assemblies thought to be important for efficient neurotransmission because they concentrate near sites of release. While previous studies have characterized...
Numerous qualitative and quantitative techniques can be used to test sensory nerves pain in both research clinical settings. The current study demonstrates a testing protocol using measure tactile sensation threshold for pressure heat portable easily accessed equipment. These equipment are ideal new laboratories clinics where cost is concern or limiting factor. We demonstrate measurement the following: cutaneous mechanical sensitivity on arms legs (von-Frey filaments), radiant contact (with...
Abstract Knowledge of efficacious dosing respective to exercise type and pain condition is extremely limited in the literature. This study aimed determine impact dose moderate intensity treadmill walking on experimentally-induced healthy human participants. Forty females were divided into 4 groups: control (no exercise), low (3×/wk), (5×/wk) or high (10×/wk). Over a 7-day period, subjects performed during assigned days. Both qualitative quantitative measures measured at baseline, trial, 24...
Tools for visualizing and manipulating protein dynamics in living cells are critical understanding cellular function. Here we leverage recently available monoclonal antibody sequences to generate a set of affinity tags labeling AMPA-type glutamate receptors (AMPARs), which mediate nearly all excitatory neurotransmission the central nervous system. These antibodies can be produced from heterologous exogenous applications or directly expressed neurons as intrabodies, where they bind their...
Abstract Recent evidence suggests that nanoorganization of proteins within synapses may control the strength communication between neurons in brain. The unique subsynaptic distribution glutamate receptors, which cluster nanoalignment with presynaptic sites release, supports this idea. However, testing it has been difficult because mechanisms controlling organization remain unknown. Reasoning transcellular interactions could position AMPA we targeted a key transsynaptic adhesion molecule...