A5090611926- Retinal Development and Disorders
- Immune Cell Function and Interaction
- Neuroscience and Neuropharmacology Research
- T-cell and B-cell Immunology
- Cellular transport and secretion
- Autophagy in Disease and Therapy
- Lipid Membrane Structure and Behavior
- Photoreceptor and optogenetics research
- Immune Response and Inflammation
- Retinal Diseases and Treatments
- MicroRNA in disease regulation
- Retinoids in leukemia and cellular processes
- Connexins and lens biology
- interferon and immune responses
- Neuroscience and Neural Engineering
- Genetic Mapping and Diversity in Plants and Animals
- Spine and Intervertebral Disc Pathology
- Nanofabrication and Lithography Techniques
- Genetic Neurodegenerative Diseases
- Cancer-related Molecular Pathways
- Protein Kinase Regulation and GTPase Signaling
- Neuroinflammation and Neurodegeneration Mechanisms
- Advanced biosensing and bioanalysis techniques
- Computational Drug Discovery Methods
- Trigeminal Neuralgia and Treatments
University of Colorado Anschutz Medical Campus
2021-2024
University of Colorado Denver
2021-2023
University of Iowa
2007-2020
University of Tennessee Health Science Center
2012-2017
The neuropeptide calcitonin gene-related peptide (CGRP) from the trigeminal ganglion has been established as a key player in pathogenesis of migraine. In this study, we provide evidence that responsiveness neuronal CGRP receptors is strongly enhanced vitro and vivo by expression human receptor activity-modifying protein-1 (hRAMP1), an obligatory subunit receptor. We first demonstrated activation on cultured neurons increased endogenous mRNA levels promoter activity. was cAMP dependent...
Purpose: Prominin-1 (Prom1) is a transmembrane glycoprotein, which expressed in stem cell lineages, and has recently been implicated cancer survival. Mutations the Prom1 gene have shown to disrupt photoreceptor disk morphogenesis cause an autosomal dominant form of Stargardt-like macular dystrophy (STGD4). Despite apparent structural role photoreceptors, its other cells retina unknown. The purpose this study investigate highly metabolically active retinal pigment epithelium (RPE). Methods:...
The sepsis-induced cytokine storm leads to severe lymphopenia and reduced effector capacity of remaining/surviving cells. This results in a prolonged state immunoparalysis, that contributes enhanced morbidity/mortality sepsis survivors upon secondary infection. impact on several lymphoid subsets has been characterized, yet its NK-cells remains underappreciated-despite their critical role controlling infection(s). Here, we observed numerical loss multiple tissues after...
Surface levels of membrane proteins are determined by a dynamic balance between exocytosis-mediated surface delivery and endocytosis-dependent retrieval from the cell surface. Imbalances in protein perturb homeostasis cause major forms human disease such as type 2 diabetes neurological disorders. Here, we found Reps1-Ralbp1-RalA module exocytic pathway broadly regulating levels. Reps1 Ralbp1 form binary complex that recognizes RalA, vesicle-bound small guanosine triphosphatases (GTPase)...
Abstract Patients who survive sepsis experience long-term immunoparalysis characterized by numerical and/or functional lesions in innate and adaptive immunity that increase the host’s susceptibility to secondary complications. The extent which tumor development/growth is affected survivors remains unknown. In this study, we show cecal ligation puncture (CLP) surgery renders mice permissive increased B16 melanoma growth weeks/months after induction. CD8 T cells provide partial protection...
Neurotransmitter receptors partition into nanometer-scale subdomains within the postsynaptic membrane that are precisely aligned with presynaptic neurotransmitter release sites. While spatial coordination between pre- and elements is observed at both excitatory inhibitory synapses, functional significance of this molecular architecture has been challenging to evaluate experimentally. Here we utilized an optogenetic clustering approach acutely alter nanoscale organization scaffold gephyrin...
Here we introduce zapalog-mediated endoplasmic reticulum trap (zapERtrap), which allows one to use light precisely trigger forward trafficking of diverse integral membrane proteins from internal secretory organelles the cell surface with single and subcellular spatial resolution. To demonstrate its utility, zapERtrap in neurons dissect where synaptic emerge at when processed through central (cell body) or remote (dendrites) pathways. We reveal rapid direct long-range centrally deep into...
Tools for visualizing and manipulating protein dynamics in living cells are critical understanding cellular function. Here we leverage recently available monoclonal antibody sequences to generate a set of affinity tags labeling AMPA-type glutamate receptors (AMPARs), which mediate nearly all excitatory neurotransmission the central nervous system. These antibodies can be produced from heterologous exogenous applications or directly expressed neurons as intrabodies, where they bind their...
Abstract Secreted amyloid-β (Aβ) peptide forms neurotoxic oligomeric assemblies thought to cause synaptic deficits associated with Alzheimer’s disease (AD). Soluble Aβ oligomers (Aβo) directly bind neurons high affinity and block plasticity mechanisms related learning memory, trigger loss of excitatory synapses eventually cell death. While Aβo toxicity has been intensely investigated, it remains unclear precisely where initially binds the surface whether sites binding relate deficits. Here,...
The purpose of this study was to characterize the potential vitamins protect retinal pig-ment epithelium (RPE) from oxidative stress (OS). We have previously shown that OS induces expression AP1 transcription factors (FOSB, CFOS and ATF3), but is modulated by pretreatment with vitamin C (200 μM). propose OS-induced can be used as a biomarker test efficacy limit impact in RPE. Here we examined E or combined A plus modulate pretreated human ARPE-19 cells (0 - 7.5 μM) (10 15 (50 100 for 3 days...
Abstract Enhanced host protection against re-infection requires generation of memory T cells sufficient quantity and functional quality. Unlike well-studied inbred mice, cell responses diverse size quality are generated following infection humans outbred mice. Thus, additional models needed that more accurately reflect variation in immune outcomes genetically populations to uncover underlying genetic causes. The Collaborative Cross (CC), a large recombinant panel is an ideal model this...