A5089295699- Neuroscience and Neuropharmacology Research
- Eating Disorders and Behaviors
- Autism Spectrum Disorder Research
- Dialysis and Renal Disease Management
- Chronic Kidney Disease and Diabetes
- Pharmacology and Obesity Treatment
- Photoreceptor and optogenetics research
- Organ Donation and Transplantation
- Renal Diseases and Glomerulopathies
- Neurogenesis and neuroplasticity mechanisms
- Lipid Membrane Structure and Behavior
- Mechanical Circulatory Support Devices
- Renal Transplantation Outcomes and Treatments
- Cardiac, Anesthesia and Surgical Outcomes
- Cardiovascular Health and Disease Prevention
- Neuroscience and Neural Engineering
- Connective tissue disorders research
- Systemic Lupus Erythematosus Research
- Erythropoietin and Anemia Treatment
- Quality and Safety in Healthcare
- Sleep and Wakefulness Research
- Nerve injury and regeneration
- Regulation of Appetite and Obesity
- Therapeutic Uses of Natural Elements
- Nitric Oxide and Endothelin Effects
University of Colorado Anschutz Medical Campus
2021-2024
University of Colorado Denver
2023
New York University
2016-2021
Neurotransmitter receptors partition into nanometer-scale subdomains within the postsynaptic membrane that are precisely aligned with presynaptic neurotransmitter release sites. While spatial coordination between pre- and elements is observed at both excitatory inhibitory synapses, functional significance of this molecular architecture has been challenging to evaluate experimentally. Here we utilized an optogenetic clustering approach acutely alter nanoscale organization scaffold gephyrin...
Despite negative association in cognition and memory, mice harboring Val66Met BDNF SNP (BDNF M/M ) exhibit enhanced motor recovery accompanied by elevated excitatory synaptic markers VGLUT1 VGLUT2 striatum contralateral to unilateral ischemic stroke. The cortico-striatal pathway is a critical gateway for plasticity of motor/gait function. We hypothesized that excitability the pathway, especially hemisphere, underlies improved recovery. To test this hypothesis, we examined key molecules...
Postsynaptic neurotransmitter receptors and their associated scaffolding proteins assemble into discrete, nanometer-scale subsynaptic domains (SSDs) within the postsynaptic membrane at both excitatory inhibitory synapses. Intriguingly, receptor SSDs are mirrored by closely apposed presynaptic active zones. These trans-synaptic molecular assemblies thought to be important for efficient neurotransmission because they concentrate near sites of release. While previous studies have characterized...
Abstract Secreted amyloid-β (Aβ) peptide forms neurotoxic oligomeric assemblies thought to cause synaptic deficits associated with Alzheimer’s disease (AD). Soluble Aβ oligomers (Aβo) directly bind neurons high affinity and block plasticity mechanisms related learning memory, trigger loss of excitatory synapses eventually cell death. While Aβo toxicity has been intensely investigated, it remains unclear precisely where initially binds the surface whether sites binding relate deficits. Here,...
Severe voluntary food restriction is the defining symptom of anorexia nervosa (AN), but anxiety and excessive exercise are maladaptive symptoms that contribute significantly to severity AN which individuals with have difficulty suppressing. We hypothesized excitability hippocampal pyramidal neurons, known anxiety, leads behavior exercise. Conversely, since glutamate transporter GLT-1 dampens neurons through uptake ambient suppression GluN2B-subunit containing NMDA receptors (GluN2B-NMDARs),...
Tools for visualizing and manipulating protein dynamics in living cells are critical understanding cellular function. Here we leverage recently available monoclonal antibody sequences to generate a set of affinity tags labeling AMPA-type glutamate receptors (AMPARs), which mediate nearly all excitatory neurotransmission the central nervous system. These antibodies can be produced from heterologous exogenous applications or directly expressed neurons as intrabodies, where they bind their...