A5017921368- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- Cancer Immunotherapy and Biomarkers
- Sepsis Diagnosis and Treatment
- vaccines and immunoinformatics approaches
- Ocular Diseases and Behçet’s Syndrome
- Retinal Diseases and Treatments
- CAR-T cell therapy research
- Breast Implant and Reconstruction
- Craniofacial Disorders and Treatments
- Cytomegalovirus and herpesvirus research
- Retinal and Optic Conditions
- Neuroinflammation and Neurodegeneration Mechanisms
- Cleft Lip and Palate Research
- Social Media in Health Education
- Hair Growth and Disorders
- Gallbladder and Bile Duct Disorders
- IL-33, ST2, and ILC Pathways
- Herpesvirus Infections and Treatments
- Burn Injury Management and Outcomes
- Vascular anomalies and interventions
- Immune responses and vaccinations
- Trauma, Hemostasis, Coagulopathy, Resuscitation
University of Minnesota
2020-2023
University of Iowa
2011-2020
Scripps Memorial Hospital
2019
Helen DeVos Children's Hospital
2011-2017
Corewell Health Blodgett Hospital
2017
Spectrum Health
2017
Michigan State University
2011-2017
Grand Rapids Community College
2011-2016
Hospital Universitario Infanta Cristina
2013-2014
Universidad Complutense de Madrid
2013-2014
Background: The safety of augmentation mammaplasty has increased dramatically in the past 20 years. Capsular contracture (CC) is most commonly reported complication mammaplasty. Objectives: authors report incidence CC a low-risk patient population after primary augmentation. Methods: retrospectively reviewed charts 856 consecutive patients who underwent between 1999 and 2009. This series did not include breast augmentation–mastopexy, secondary augmentation, revision, and/or reconstruction....
Members of the NLR family can assemble inflammasome complexes with adaptor protein ASC and caspase-1 that result in activation release IL-1β IL-18. Although NLRC4 is known to have a protective role tumorigenesis, there an increased appreciation for inflammasome-independent actions NLRC4. Here, we utilized syngeneic subcutaneous murine model B16F10 melanoma explore tumor suppression. We found NLRC4-deficient mice exhibited enhanced growth was independent components caspase-1. Nlrc4 expression...
Abstract Mortality from sepsis frequently results secondary infections, and the extent to which affects pathogen-specific memory CD8 T cell responses remains unknown. Using cecal ligation puncture model of polymicrobial sepsis, we observed rapid apoptosis pre-existing cells after induction that led a loss in cell–mediated protection. Ag sensitivity (functional avidity) Ag-driven expansion were decreased further contributing immunity. Moreover, Ag-independent bystander activation response...
Memory CD8 T cells confer increased protection to immune hosts upon secondary viral, bacterial, and parasitic infections. The level of provided depends on the numbers, quality (functional ability), location memory present at time infection. While primary can be maintained for life host, full extent phenotypic functional changes that occur over after initial antigen encounter remains poorly characterized. Here we show critical properties circulating cells, including location, phenotype,...
Abstract The extent to which the progeny of one primary memory CD8 T cell differs from naive same specificity remains an unresolved question. To explore cell-autonomous functional differences between and cells that are not influenced by in priming environment, experimental model has been developed physiological numbers both populations were cotransferred into hosts before Ag stimulation. Interestingly, undergo greater expansion than do after various infections or immunizations. intrinsic...
Recent work has suggested that current mouse models may underrepresent the complexity of human immune responses. While most immunology studies utilize inbred strains, it is unclear if conclusions drawn from mice can be extended to all strains or generalized humans. We recently described a "surrogate activation marker" approach could used track polyclonal CD8 T cell responses in and outbred noted substantial discord magnitude kinetics individual following infection. However, how memory...
Induction of memory CD8 T cells is important for controlling infections such as malaria HIV/AIDS, and cancer immunotherapy. Accurate assessment antigen (Ag)-specific T-cells critical vaccine optimization defining correlates protection. However, conditions determining Ag-specific T-cell responses ex-vivo using ICS may be variable, especially in humans with complex antigens. Here, we used an attenuated whole parasite model various experimental mice to show that the duration antigenic...
Repeated infections and experimental prime-boost regimens frequently result in the generation of secondary (2°) CD8(+) T-cell responses. In contrast to primary (1°) T cells, parameters that influence abundance phenotype 2° effector memory populations are largely unknown. Here, we analyze impact different booster infections, Ag curtailment, systemic inflammation on quality quantity We show similar 1° responses, is critically dependent nature infectious pathogen inflammatory milieu early after...
Uropathogenic
Abstract In experimental models in which the Ag-stimulation history of memory CD8 T cell populations was clearly defined (adoptive transfer a known number TCR-transgenic cells), all facets ensuing responses, including proliferative expansion, duration and extent contraction, diversification transcriptomes, life-long survival, were dependent on prior Ag encounters. However, to sequential adoptive-transfer reflect physiological scenario cells are generated by repetitive challenges individual...
Memory CD8 T cells can be activated and induced to produce cytokines increase stores of cytolytic proteins not only in response cognate antigen (Ag), but also inflammatory (bystander responses). Importantly, bystander memory cell functions have been shown dependent upon fitness, since exhausted diminished capacity respond cues. While it is known that functional abilities, including ability Ag, change with time after initial Ag encounter multiple Ag-stimulations (e.g. primary vs. tertiary...
Memory CD8 T cells provide protection to immune hosts by eliminating pathogen-infected during re-infection. While parameters influencing the generation of primary (1°) are well established, factors controlling development secondary (2°) cell responses remain largely unknown. Here, we address mechanisms involved in and 2° memory (M) cells. We observed that time at which 1° M enter into response impacts their fate differentiation Late-entry an (relative onset infection) not only facilitated...
Abstract One goal of immunization is to generate memory CD8 T cells sufficient quality and quantity confer protection against infection. It has been shown that cell differentiation in vivo controlled, at least part, by the amount duration infection, Ag, inflammatory cytokines present early after initiation response. In this study, we used models anti-vectorial immunity investigate impact pre-existing on development vector-induced primary responses. We showed existing influences magnitude...
Abstract Memory CD8 T-cell activation, including expression of IFN-γ and granzymeB, can be induced by antigen (Ag)-dependent signals through the T-cell-receptor, or pathogen-derived inflammatory cytokines in an Ag-independent manner. Recent studies have come to conflicting results regarding contributions Ag and/or inflammation memory activation. Additionally, research has indicated that inflammation-driven responses during un-related infections (bystander activation) potential provide...
The tongue-lip adhesion has undergone several modifications in an attempt to reduce surgical complications and failure rates. Current techniques rely on the use of a button at tongue base for suspension, which raises concerns about possible aspiration interference with oral motor function bottle-feeding. A new technique is proposed that adds suspension standard adhesion.A total 22 patients Pierre Robin sequence who received via were reviewed. differs from approach by suture weave across...
Successful vaccination strategies offer the potential for lifelong immunity against infectious diseases and cancer. There has been increased attention regarding limited translation of some preclinical findings generated using specific pathogen-free (SPF) laboratory mice to humans. One reason difference between clinical lies in maturation status immune system at time challenge. In this study, we used a "dirty" mouse model, where SPF were cohoused (CoH) with pet store permit microbe transfer...