A5101600732- HIV Research and Treatment
- Immune Cell Function and Interaction
- interferon and immune responses
- Signaling Pathways in Disease
- Cytomegalovirus and herpesvirus research
- Advanced Drug Delivery Systems
- Reproductive System and Pregnancy
- IL-33, ST2, and ILC Pathways
- Mosquito-borne diseases and control
- Viral Infections and Vectors
- RNA Interference and Gene Delivery
- HIV/AIDS drug development and treatment
- Respiratory viral infections research
- Pleural and Pulmonary Diseases
- T-cell and B-cell Immunology
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Viral gastroenteritis research and epidemiology
- Advanced biosensing and bioanalysis techniques
- Aquaculture disease management and microbiota
- Influenza Virus Research Studies
- Virus-based gene therapy research
- Virology and Viral Diseases
- Electrospun Nanofibers in Biomedical Applications
- Lung Cancer Treatments and Mutations
- Receptor Mechanisms and Signaling
University of Massachusetts Chan Medical School
2016-2024
Seoul National University
2020-2021
Yonsei University
2016
Chonnam National University Hospital
2013
Tripartite motif-containing protein 5α (TRIM5α) is a cellular antiviral restriction factor that prevents early events in retrovirus replication. The activity of TRIM5α thought to be limited retroviruses as result highly specific interactions with capsid lattices. In contrast this current understanding, we show both human and rhesus macaque suppress replication flaviviruses. Multiple viruses the tick-borne encephalitis complex are sensitive TRIM5α-dependent restriction, but mosquito-borne...
HIV-1-infected people who take drugs that suppress viremia to undetectable levels are protected from developing AIDS. Nonetheless, HIV-1 establishes proviruses in long-lived CD4+ memory T cells, and perhaps other cell types, preclude elimination of the virus even after years continuous antiviral therapy. Here we show provirus activates innate immune signaling isolated dendritic macrophages, cells. Immune activation requires transcription expression CRM1-dependent, Rev-dependent,...
The HIV-1 capsid protein makes up the core of virion and plays a critical role in early steps HIV replication. Due to its exposure cytoplasm after entry, is target for host cell factors that act directly block infection such as TRIM5α MxB. Several proteins also play facilitating infection, including protection from recognition by restriction factors. Through an unbiased screening approach, called HIV-CRISPR, we show CPSF6-binding deficient, N74D mutant sensitive mediated human TRIM34, close...
ABSTRACT Cyclophilin A (CypA) binds to the HIV-1 capsid facilitate reverse transcription and nuclear entry counter antiviral activity of TRIM5α. Interestingly, recent studies suggest that enters nucleus an infected cell uncoats prior integration. We have previously reported protein regulates Therefore, we probed whether CypA-capsid interaction also this post-nuclear step. First, challenged CypA-expressing (CypA +/+ ) CypA-depleted -/- cells with quantified levels provirus. CypA-depletion...
pH-Responsive P(MAA-co-EGMA) hydrogel microparticles were prepared and their feasibility as intelligent delivery carriers was evaluated. synthesized via dispersion photopolymerization. There a drastic change in the swelling ratio of at pH ~ 5 and, amount MAA increased, increased above 5. The loading efficiency ascorbic acid into affected more by degree than electrostatic interaction between loaded acid. showed pH-sensitive release behavior. Thus, 4 almost none permeated through skin while 6...
In this work, highly osmotic oxidized sucrose-crosslinked polyethylenimine (SP2K) polymers were developed for gene delivery systems, and the transfection mechanism is examined. First, periodate-oxidized sucrose 2K (PEI2K) crosslinked with various feed ratios via reductive amination. The synthesis was confirmed by 1H NMR FTIR. synthesized SP2K could form positively charged (~40 mV zeta-potential) nano-sized (150–200 nm) spherical polyplexes plasmid DNA (pDNA). They showed lower cytotoxicity...
Streptococcus parauberis is utilized as an oral vaccine by first inactivating the cells with formalin to produce formalin- killed (FKC) and then encapsulating them polymer beads consisting of a cross-linked alginate-Ca2+ network. The encapsulation efficiency media-dependent release are controlled pre-treating FKC two types clay nanoparticles: kaolinite (KA) layered double hydroxide (LDH). addition LDH induced large agglomerates FKC, KA enhanced dispersion FKC. differences in dispersibility...
HIV-1-infected people who take medications that suppress viremia, preserve CD4+ T cells, and prevent AIDS, have chronic inflammation with increased cardiovascular mortality. To investigate the etiology of this inflammation, effect HIV-1 on innate lymphoid cells (ILCs) NK was examined. Homeostatic ILCs in blood intestine were depleted permanently. skewed towards a memory subset. Cytokines are elevated during infection reproduced both abnormalities ex vivo. Pseudotime analysis single cell...
Abstract Cyclophilin A (CypA) promotes HIV-1 infection by facilitating reverse transcription, nuclear entry and countering the antiviral activity of TRIM5α. These multifunctional roles CypA are driven its binding to viral capsid. Interestingly, recent studies suggest that capsid lattice enters nucleus an infected cell uncoats just before integration. Therefore, we tested whether CypA-capsid interaction regulates post-nuclear steps infection, particularly First, challenged CypA-expressing...
The capsid (CA) protein lattice of HIV-1 and other retroviruses encases viral genomic RNA regulates steps that are essential to retroviral invasion target cells, including reverse transcription, nuclear trafficking, integration cDNA into host chromosomal DNA 1 . Cyclophilin A (CypA), the first cellular reported bind CA 2 , has interacted with invading lentiviruses related for millions years 3–7 Disruption CA-CypA interaction decreases infectivity in human cells 8–12 but stimulates non-human...
Drugs that inhibit HIV-1 replication and prevent progression to AIDS do not eliminate proviruses from the chromosomes of long-lived CD4 + memory T cells. To escape eradication by these antiviral drugs, or host immune system, exploits poorly defined factors silence provirus transcription. These same factors, though, must be overcome all retroviruses, including other primate immunodeficiency viruses, in order activate transcription produce new virus. Here we show Vpx Vpr, proteins a wide range...
Abstract The HIV-1 capsid protein makes up the core of virion and plays a critical role in early steps HIV replication. Due to its exposure cytoplasm after entry, is target for host cell factors that act directly block infection such as TRIM5 MxB. Several proteins also play facilitating infection, including protection from recognition by restriction factors. Through an unbiased screening approach, called HIV-CRISPR, we show Cyclophilin A-binding deficient P90A mutant becomes...
ABSTRACT HIV-1-infected people who take drugs that suppress viremia to undetectable levels are protected from developing AIDS. Nonetheless, these individuals have chronic inflammation associated with heightened risk of cardiovascular pathology. HIV-1 establishes proviruses in long-lived CD4 + memory T cells, and perhaps other cell types, preclude elimination the virus even after years continuous antiviral therapy. Though majority persist during therapy defective for production infectious...
Abstract Tripartite motif-containing protein 5α (TRIM5α) functions as a cellular antiviral restriction factor with exquisite specificity towards the capsid lattices of retroviruses. The relative avidity TRIM5α binding to retrovirus capsids directly impacts primate species susceptibility infection, but role is thought limited In contrast this current understanding, here we show that both human and rhesus possess potent function against specific flaviviruses through interaction viral protease...