Oxidative stress as well inflammation processes are engaged in diabetic vascular complications. Rosmarinic acid, a natural phenol antioxidant carboxylic was found to have multiple biological activity, including anti-inflammatory and antitumour effects, which consequence of its inhibition the inflammatory reactive oxygen species scavenging. The aim this work study effects rosmarinic acid administration on impairment induced by experimental diabetes rats.Diabetes streptozocin (3 × 30 mg/kg...

Abstract In this study, a role of cell loss due to necroptosis and its linkage with pyroptosis in organ damage under the conditions pulmonary arterial hypertension (PAH) was examined. Monocrotaline (MCT) used induce PAH Wistar rats, depending on severity disease progression, they were further divided into two subgroups: MCT group—sacrificed 4 weeks after administration ptMCT group—prematurely sacrificed rapid deterioration vital functions (on Day 24,11 ± 0,7). The elevation respiratory rate...

In view of previously reported increased capacity for nitric oxide production, we suggested that l-arginine (ARG), the synthase (NOS) substrate, supplementation would improve cardiac function in isoproterenol (ISO)-induced heart failure. Male Wistar rats were treated with ISO 8 days (5 mg/kg/day, i.p.) or vehicle. ARG was given to control (ARG) and ISO-treated (ISO+ARG) water (0.4 g/kg/day). administration associated 40% mortality, ventricular hypertrophy, decreased rate, left dysfunction,...

Abstract Background In spite of disrupted repolarization diabetic heart, some studies report less tendency heart to develop ventricular arrhythmias suggesting effective compensatory mechanism. We hypothesized that myocardial alterations in HCN2 and HCN4 channels occur under hyperglycaemia. Methods Diabetes was induced rats using a single injection streptozotocin (STZ; 55 mg/kg body weight, i.p.). Basal ECG measured. Expression mRNA for HCN channels, potassium microRNA 1 133a were measured...

Abstract Right ventricular (RV) failure is the primary cause of death in pulmonary arterial hypertension (PAH). We hypothesized that heart‐relevant microRNAs, myomiRs (miR‐1, miR‐133a, miR‐208, miR‐499) and miR‐214, can have a role right ventricle development PAH. To mimic PAH, male Wistar rats were injected with monocrotaline (MCT, 60 mg/kg, s.c.); control group received vehicle. MCT divided into two groups, based on clinical presentation: terminated 4 weeks after administration prematurely...

Abstract We tested the hypothesis that daunorubicin ( DAU ) cardiotoxicity alters expression of cytokines involved in stem cell migration and homing. Male Wistar rats were treated with to induce acute cardiomyopathy (6 × 3 mg/kg, i.p., every 48 hr, ‐A) or subchronic (15 i.v., ‐C). The left ventricle was catheterized. animals killed hr 8 weeks ‐C) after last dose . Expression foetal genes (Nppa, Nppb), isomyosins (Myh6, Myh7), sources oxidative stress (Abcb8, gp91phox), (Sdf‐1, Cxcr4, Scf,...

Daunorubicin (DAU) downregulates cytokines promoting stem cell migration and homing into the heart, reducing cardiac regeneration after anticancer chemotherapy. Pegfilgrastim (PFIL) protects from DAU-induced neutropenia but its cardioprotective potential remains unclear. We tested whether pegfilgrastim a dipeptidyl peptidase-4 inhibitor linagliptin, enhancers of cells homing, would improve DAU-cardiomyopathy.DAU (7.5 mg/kg, i.v.) was administered to male Wistar rats induce cardiotoxicity....

This study is designed to address the connection between antidepressant and antipsychotic-induced hepatotoxicity with pharmacogenetic epigenetic indicators, using a novel combined approach of CYP450 polymorphism determination early liver injury detection via microRNA testing. The multi-centric retrospective case-control in Slovakia involves 151 cases signs 604 controls without. Participants will be tested for selected CYP450, UGT1A1 polymorphisms, microRNAs. Anticipated findings test if...

Despite the fact that vessels have sparse cholinergic innervation, acetylcholine (ACh), primary neurotransmitter of parasympathetic nervous system, has been commonly used in physiological experiments to assess vascular function. ACh is hydrolyzed by two cholinesterases (ChE), namely acetylcholin-esterase and butyrylcholinesterase (BChE). However, little known about these enzymes blood vessels. The aim project was characterize expression activity ChE rat aorta. As effect on tone depends...

Objective: Pulmonary arterial hypertension (PAH) is a fatal incurable disease with complicated and lengthy diagnosis. However, its early detection prerequisite for better treatment longer survival of patients. Therefore, the goal not only to find new procedures, but also ways diagnose PAH faster easier by biomarkers specifically involved in progression. Tissues cardiovascular system produce, among others, hsa-mir-126-3p (endothelium) hsa-mir-423-5p (cardiac muscle). These microRNAs are...

Objective: Epoxyeicosatrienoic acids (EETs) are a group of auto/paracrine mediators derived from arachidonic acid with vasculoprotective and vasodilatory properties. Hydroxyeicosatetraenoic (HETEs) act predominantly as physiological antagonists EETs. Our objective was to compare expression CYP enzymes involved in production degradation EETs HETEs two animal models different levels glucose metabolism impairment - intolerance model (GI) diabetic (DIA). Design method: 12–13 weeks old male...

Abstract Drug‐induced long QT syndrome (LQTS) and Torsades de Pointes (TdP) are serious concerns in drug development. Although rats a useful scientific tool, their hearts, unlike larger species, usually do not respond to torsadogenic drugs. Consequently, resistance drug‐induced arrhythmias is poorly understood. Here, we challenged with rapid delayed rectifier current (Ikr)‐inhibiting antibiotic clarithromycin (CLA), loop diuretic furosemide (FUR) or combination (CLA + FUR), examined...

Aim: Circulating microRNA-21 (miR-21) has been utilized as a diagnostic tool in the assessment of heart failure (HF). Blood constitution may be altered when HF occurs and miR-21 affect hematopoiesis. Sample hemolysis influence determination circulating miRNAs, challenging use miRNAs. Methods: We examined relationship between blood measurements levels ambulant chronic patients with reduced ejection fraction (HFrEF; n = 19). Healthy volunteers (n 11) served controls. Serum were measured...

Caveolin-1 (cav-1) plays a role in pulmonary arterial hypertension (PAH). Monocrotaline (MCT)-induced PAH is characterized by loss of cav-1 arteries; however, less known regarding its the hypertrophied right ventricle (RV). We aimed to characterize and Hsp90 RV MCT-induced their impact on endothelial nitric oxide synthase (eNOS). Additionally, we focused restoration expression with pioglitazone administration.Male 12-week-old Wistar rats were injected subcutaneously monocrotaline (60 mg/kg)....

Objective: NGF is a member of the neurotrophin family, whose role has been described in development and persistence pulmonary hypertension (PH). Let-7 family group microRNAs that targets downregulates its expression. On other hand, microRNA miR-21, shown to support signalling, expression increased after stimulation neuronal cells. Furthermore, miR-21 was reported contribute proliferation migration lung vascular smooth muscle cells decrease BMPR-II In this study, we examined these regulation...

Objective: Acetylcholine-induced (ACh) relaxation is routinely used to evaluate endothelial function. Via muscarinic M3 receptors (M3MR), ACh stimulates synthesis of NO and EDHF resulting in endothelium-dependent relaxation. Vascular cholinergic system remains practically unexplored. The objective this project was determine the localization mRNA acetylcholinesterase (AChE), butyrylcholinesterase (BChE), MR, cholinesterase anchoring proteins PRiMA1, ColQ1 within endothelium smooth muscle (SM)...

Objective: Cytokines and growth factors could potentially regulate migration homing of stem cells, subsequently regeneration injured heart in acute subchronic daunorubicin (DAU) cardiomyopathy. Design method: Male Wistar rats (10–12 weeks old) were used to induce (6xi.p. DAU, 3 mg/kg every 48 h) (1x i.v. 15 mg/kg) DAU Controls received vehicle. Hemodynamic parameters measured by left ventricular catheterization. Animals sacrificed 2 (acute) 8 (subchronic model) after the first dose DAU....

Objective: Pleural effusions (PLEF) in patients with pulmonary arterial hypertension (PAH) co-diagnosed isolated right-sided heart failure (RHF) have been reported to be associated increased mortality. We hypothesized that alterations of transporters responsible for alveolar fluid clearance contributes accumulation lungs and secondary formation PLEF. This was examined a model PAH including administration riociguat (RIO) as stimulator sGC may cGMP-dependent inhibition epithelial sodium...

Purpose: Our previous studies suggested an increased capacity for NO production in isoproterenol (ISO)-induced heart failure. Supplementation by L-arginine (ARG), the substrate of nitric oxide synthases, had a positive effect human We examined its effects on cardiac function ISO-induced Methods: 18-week-old male Wistar rats were treated with ISO 8 days (ISO; 5 mg/kg/d, i.p.) or vehicle. ARG was given to control (ARG) and ISO-treated (ISO+ARG) water (0.4 g/kg/d). ECG hemodynamics measured...

Krenek, P.; Pivackova, L.; Cernecka, H.; Turcekova, K.; Olvedy, M.; Klimas, J.; Ochodnicky, Gajdacova, B.; Adameova, A.; Kyselovic, J. Author Information

Objective: Neprilysin is a zinc metallopeptidase that cleaves and inactivates several signalling peptides peptide hormones including natriuretic (NPs). Plasma concentration of NT-proBNP (which not cleaved by neprilysin) one principal diagnostic tests in heart failure (HF) diagnostics. We hypothesized neprilysin levels might provide additional performance treated chronic HF patients when mineralocorticoid antagonist (MR) added. Design method: Patients for with addition MR (duration therapy 37...