A5065014247- Nitric Oxide and Endothelin Effects
- Sulfur Compounds in Biology
- Eicosanoids and Hypertension Pharmacology
- Renin-Angiotensin System Studies
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Neuroscience of respiration and sleep
- Cardiovascular Disease and Adiposity
- Cardiovascular Health and Disease Prevention
- Heart Rate Variability and Autonomic Control
- Adipose Tissue and Metabolism
- Blood Pressure and Hypertension Studies
- Electron Spin Resonance Studies
- Selenium in Biological Systems
- Biochemical effects in animals
- Receptor Mechanisms and Signaling
- Diet, Metabolism, and Disease
- Mast cells and histamine
- Antioxidant Activity and Oxidative Stress
- Sodium Intake and Health
- Hydrogen's biological and therapeutic effects
- Hormonal Regulation and Hypertension
- Alcohol Consumption and Health Effects
- Vitamin C and Antioxidants Research
- Trace Elements in Health
- Anesthesia and Neurotoxicity Research
Slovak Academy of Sciences
2016-2025
Institute of Normal and Pathological Physiology of the Slovak Academy of Sciences
2015-2024
Centre of Experimental Medicine of the Slovak Academy of Sciences
2011-2024
Slovak Astronomical Society at the Slovak Academy of Sciences
2012
Abstract Since the identification of alternative angiotensin converting enzyme ( ACE )2/Ang‐(1‐7)/Mas receptor axis, renin‐angiotensin system RAS ) is a new complex target for pharmacological intervention. We investigated expression components in heart and kidney during development hypertension its perinatal treatment with losartan young spontaneously hypertensive rats SHR ). Expressions genes were studied by RT ‐ PCR left ventricle rats: normotensive Wistar, untreated SHR, treated since...
<title>Abstract</title> Metabolic syndrome is a growing global health burden, resulting in an urgent need for new therapeutic strategies. We evaluated the effects of 3 weeks treatment with slow-releasing H<sub>2</sub>S donor GYY-4137 on adiposity, systolic blood pressure (sBP), plasma biochemical indices, vascular function and nitric oxide (NO) hydrogen sulfide (H<sub>2</sub>S) pathways isolated thoracic aortas (TAs) mesenteric arteries (MAs) from spontaneously hypertensive rats (SHRs) fed...
Several studies have already confirmed the specific vasomotor effect of hydrogen sulfide (H2S) and its interaction with nitric oxide (NO) system in normotensive rats, but results spontaneously hypertensive rats (SHRs) are limited. aim this study was to describe age- blood pressure-dependent effects endogenous NO exogenous Na2S their responses thoracic aorta (TA) Wistar SHRs. systolic pressure (sBP), vasoactivity, NO-synthase (NOS) expression activity, cystathionine gamma-lyase (CSE)...
Background: Quercetin (QCT) was shown to exert beneficial cardiovascular effects in young healthy animals. The aim of the present study determine benefits QCT older, 6-month and 1-year-old Zucker diabetic fatty (ZDF) rats (model type 2 diabetes). Methods: Lean (fa/+) obese (fa/fa) ZDF both ages were treated with for 6 weeks (20 mg/kg/day). Isolated hearts exposed ischemia-reperfusion (I/R) injury (30 min/2 h). Endothelium-dependent vascular relaxation measured isolated aortas. Expression...
The effect of a 10-day-long treatment with taxifolin (TAX, 20 mg/kg/day p.o.) was investigated on spontaneously hypertensive rats (SHRs) focus the vascular functions isolated femoral arteries and thoracic aortas. TAX reduced blood pressure in SHRs. In arteries, increased acetylcholine-induced relaxation, maximal NA-induced contraction, endothelium-dependent contraction (EDC); however, had no reactivity addition, elevated total nitric oxide synthase (NOS) activity iNOS protein expression but...
Perivascular adipose tissue (PVAT) and hydrogen sulfide (H2S) play important roles in the modulation of vasoactive responses can interfere with ethiopathogenesis essential hypertension. The aim this study was to evaluate mutual relationship between PVAT H2S (endogenously produced, exogenous) isolated mesenteric arteries (MA) adult normotensive (Wistar) spontaneously hypertensive rats (SHR). In SHR, hypertension associated cardiac hypertrophy increased contractility; however, there were no...
The objective of this study was to investigate the vasoregulatory role perivascular adipose tissue (PVAT) and its mutual interaction with endogenous exogenous H2S in thoracic aorta (TA) adult normotensive Wistar rats spontaneously hypertensive (SHRs). In SHRs, hypertension associated cardiac hypertrophy increased contractility. Regardless strain, PVAT revealed an anticontractile effect; however, worsened endothelial-dependent vasorelaxation. Since produced by both vascular wall had a...
As a part of the nitroso signalling pathway, nitroso-compounds serve as stores and carriers NO; sulphide bound sulfane-sulphur compounds H 2 S.Here we hypothesise coupled sulphide-nitroso in which S plays main role.H releases NO from endogenous S-nitroso-compounds nitrosocysteine, nitroso-acetylcysteine nitroso-albumin.Relaxation noradrenaline-precontracted aortic rings by is also enhanced presence nitroso-albumin, may implicate involvement pathway.Pretreatment albumin, cysteine,...
We studied the effects of H2S donor Na2S on mean arterial blood pressure (MAP) and heart breathing rates anesthetized Wistar rats in presence absence captopril. Bolus administration (1-4 µmol/kg) into right jugular vein transiently decreased increased rates; at 8-30 µmol/kg, had a biphasic effect, decreasing increasing MAP, while rate rate. These results may indicate independent mechanisms by which influences MAP rates. The effect was less pronounced captopril (2 µmol/l), that...
PPAR γ receptor plays an important role in oxidative stress response. Its agonists can influence vascular contractility experimental hypertension. Our study was focused on the effects of a agonist pioglitazone (PIO) blood pressure regulation, vasoactivity vessels, and redox-sensitive signaling at central (brainstem, BS) peripheral (left ventricle, LV) levels young prehypertensive rats. 5-week-old SHR were treated either with PIO (10 mg/kg/day, 2 weeks) or saline using gastric gavage....
The aim of this study was to evaluate the mutual relationship among perivascular adipose tissue (PVAT) and endogenous exogenous H2S in vasoactive responses isolated arteries from adult normotensive (Wistar) rats hypertriglyceridemic (HTG) rats, which are a nonobese model metabolic syndrome. In HTG mild hypertension associated with glucose intolerance, dyslipidemia, increased amount retroperitoneal fat, arterial contractility, endothelial dysfunction wall injury, accompanied by decreased...
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infects host cells through angiotensin-converting enzyme 2 (ACE2). Concurrently, the product of ACE2 action, angiotensin 1-7 (Ang 1-7), binds to Mas receptors within cardiovascular system and provides protective effects. Therefore, it is crucial reveal role inhibition, especially pre-existing pathologies. In our study, we imitated action SARS-CoV-2 in organisms using low dose inhibitor MLN-4760 with aim investigating what degree...
Phthalic selenoanhydride (R-Se) solved in physiological buffer releases various reactive selenium species including H2Se. It is a potential compound for Se supplementation which exerts several biological effects, but its effect on the cardiovascular system still unknown. Therefore, herein we aimed to study how R-Se affects rat hemodynamic parameters and vasoactive properties isolated arteries. The right jugular vein of anesthetized Wistar male rats was cannulated IV administration R-Se....
Garlic, onion and leek have beneficial effects in treatment of numerous health disorders. The aim the present study was to investigate underlying molecular mechanisms. To test potency aqueous garlic, extracts release NO from GSNO we measured oxidation product, NO(2)-, by Griess reagent method. Further, studied ability garlic extract relax noradrenaline-precontracted rat aortic rings presence on electrical properties heart intracellular chloride channels. We observed that: i) released order:...
The brain stem contains important nuclei that control cardiovascular function via the sympathetic nervous system (SNS), which is strongly influenced by nitric oxide. Its biological activity also largely determined oxygen free radicals. Despite many experimental studies, role of AT1R-NAD(P)H oxidase-superoxide pathway in NO-deficiency not yet sufficiently clarified. We changes radical signaling and antioxidant detoxification response young adult Wistar rats during chronic administration...
The status of nitric oxide (NO) in spontaneously hypertensive rats (SHR) is unclear and its bioavailability may be affected by imbalance with reactive oxygen species.We studied cardiovascular effects an NO donor, pentaerythrityl tetranitrate (PETN) SHR.We used Wistar rats, SHR treated PETN (100 mg/kg/day).After six weeks, myocardium aorta from each group were taken for biochemical iliac artery functional morphological study.Long-term administration to increased cGMP level platelets did not...