A5011657635- Nitric Oxide and Endothelin Effects
- Mast cells and histamine
- Cancer and Skin Lesions
- Receptor Mechanisms and Signaling
- Monoclonal and Polyclonal Antibodies Research
- Ion channel regulation and function
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Cardiac electrophysiology and arrhythmias
- Glycosylation and Glycoproteins Research
- Cell Adhesion Molecules Research
- Molecular Biology Techniques and Applications
- Cancer Cells and Metastasis
- ATP Synthase and ATPases Research
- Medical and Biological Ozone Research
- Breast Lesions and Carcinomas
- Cardiac Ischemia and Reperfusion
- Exercise and Physiological Responses
- Eicosanoids and Hypertension Pharmacology
- Cancer Immunotherapy and Biomarkers
- Pancreatitis Pathology and Treatment
- Protein Kinase Regulation and GTPase Signaling
- Spaceflight effects on biology
- Salivary Gland Tumors Diagnosis and Treatment
- Cellular transport and secretion
- Pancreatic function and diabetes
Institut für Hämatopathologie Hamburg
2013-2024
Russian New University
2023-2024
Peoples' Friendship University of Russia
2022-2024
University Hospital Münster
2004-2019
Martin Luther University Halle-Wittenberg
2019
University of Regensburg
2019
University of Gothenburg
2013
Albertinen Diakoniewerk
2013
Institut Kurz
2009-2010
University of Münster
2001-2010
The current protocols for blocking background staining in immunohistochemistry are based on conflicting reports. Background is thought to occur as a result of either non-specific antibody (Ab) binding endogenous Fc receptors (FcRs) or combination ionic and hydrophobic interactions. In this study, cell tissue samples were processed according routine with without step (goat serum BSA). Surprisingly, no Abs showed any propensity leading staining, implying that FcRs do not retain their ability...
Current classification systems in proliferative mammary gland pathology are based on a two-cell system, recognizing only glandular and myoepithelial lines of differentiation. A third cell type has recently been characterized normal breast tissue by double-immunofluorescence analysis to express cytokeratin 5 (Ck5) only. These cells were shown represent progenitor or adult stem that give rise the lineage. The double-labelling technique applied characterize spectrum intraductal epithelial...
The concept of endothelium-derived relaxing factor (EDRF) put forward in 1980 by Furchgott and Zawadzki implies that nitric oxide (NO) produced NO synthase (NOS) the endothelium diffuses to underlying vascular smooth muscle, where it modulates tone as well muscle cell (VSMC) proliferation increasing cGMP formation with subsequent activation cGMP-dependent protein kinase. According this concept, VSMC do not express NOS themselves. This attractive, simple scheme is now under considerable...
The aim of this study was to define whether or not the impaired expression CD44, E-cadherin (E-cad), and beta-catenin (beta-cat) correlates with clinical evolution prognosis oral cancer. Ninety-three primary squamous cell carcinomas (OSCCs) tumour-adjacent normal and/or dysplastic mucosa, 30 associated metastases, 12 recurrences were immunostained for CD44s, -v3, -v4, -v5, -v6, -v7, -v9, E-cad, beta-cat. In non-neoplastic epithelium, all molecules investigated constitutively expressed in...
Reversible protein phosphorylation is an essential regulatory mechanism in many cellular functions. In contrast to kinases, the role and regulation of phosphatases has remained ambiguous. To address this issue, we generated transgenic mice that overexpress catalytic subunit alpha phosphatase 2A (PP2A) (PP2Acalpha) heart driven by alpha-myosin heavy chain promoter. Overexpression PP2Acalpha gene led increased levels transgene both at RNA levels. This was accompanied a significant increase...
The CD4+ natural killer (NK)T cells in the liver are potent IL-4 producers and hence may promote Th2 cell development. Following Mycobacterium bovis bacillus Calmette Guérin (BCG) infection, IL-4-producing NKT become undetectable mononuclear of normal density (interface between 40 70 % Percoll) by flow cytometry. present study shows that M. BCG infection changes shifts cytokine production from to IFN-γ. number NK1+ TCRα / βintermediate increased low-density fraction (< Percoll gradient)...
In an integrative approach, we studied the role of histamine H<sub>2</sub> receptors in mouse heart. We noted that histamine, added cumulatively to organ bath, failed affect force contraction left atrial preparations and did not change spontaneous heart rate right from wild-type mice. By contrast, same mice overexpressed human receptor a cardiac-specific way, exerted concentration- time-dependent positive inotropic chronotropic effects. Messenger RNA was only detected transgenic Likewise,...
Syringomatous tumour of the nipple and low-grade adenosquamous carcinoma (LGAdSC) breast are regarded as distinct entities. To clarify nature these two lesions, we compared expression different lineage/differentiation markers in 12 syringomatous tumours nipple, nine LGAdSCs, normal epithelium.Using triple immunofluorescence labelling quantitative RT-PCR for keratins, p63, smooth muscle actin, demonstrated that LGAdSC contain p63+/K5/14+ cells, K10+ squamous K8/18+ glandular with intermediary...
Histamine is metabolized by several enzymes in vitro and vivo . The relevance of this metabolism the mammalian heart unclear. However, histamine can exert positive inotropic effects (PIE) chronotropic (PCE) humans via H 2 -histamine receptors. In transgenic mice (H -TG) that overexpress human receptor cardiomyocytes but not wild-type littermate (WT), induced PIE PCE isolated left or right atrial preparations. These -TG were used to investigate putative degrading heart. Histidine, precursor...
The mechanisms of regeneration for the fibrous component connective tissue dermis are still insufficiently studied. aim this study was to evaluate effectiveness use molecular hydrogen on local therapy a II degree burn wound with intensification collagen fibrillogenesis in skin. We analyzed involvement mast cells (MCs) fibers using water high content and therapeutic ointment cell wounds. Thermal burns led an increase skin MC population, accompanied by systemic rearrangement extracellular...
Serotonin (5-HT) exerts pleiotropic effects in the human cardiovascular system. Some of are thought to be mediated via 5-HT 4 receptors, which expressed atrium and ventricular tissue. However, a true animal model study these receptors more detail has been hitherto lacking. Therefore, we generated, for first time, transgenic (TG) mouse with cardiac myocyte-specific expression receptor. RT-PCR immunohistochemistry revealed receptor at mRNA protein levels. Stimulation isolated preparations by...
Dopamine can exert effects in the mammalian heart via five different dopamine receptors. There is controversy whether receptors increase contractility human heart. Therefore, we have generated mice that overexpress D