A5003370400- Carcinogens and Genotoxicity Assessment
- Air Quality and Health Impacts
- Toxic Organic Pollutants Impact
- Nanoparticles: synthesis and applications
- Graphene and Nanomaterials Applications
- Indoor Air Quality and Microbial Exposure
- Air Quality Monitoring and Forecasting
- Inhalation and Respiratory Drug Delivery
- Vehicle emissions and performance
- Animal testing and alternatives
- 3D Printing in Biomedical Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Anesthesia and Neurotoxicity Research
- Nutrition, Genetics, and Disease
- Mesenchymal stem cell research
- Glutathione Transferases and Polymorphisms
- Spaceflight effects on biology
- Inflammatory mediators and NSAID effects
- Medical and Biological Ozone Research
- Nanoparticle-Based Drug Delivery
- Electrohydrodynamics and Fluid Dynamics
- Drug Solubulity and Delivery Systems
- Retinal Development and Disorders
- Diatoms and Algae Research
- Wound Healing and Treatments
Czech Academy of Sciences, Institute of Experimental Medicine
2017-2025
Charles University
2018-2022
Swansea University
2020
Czech Academy of Sciences
2017
Following advancements in the field of genotoxicology, it has become widely accepted that 3D models are not only more physiologically relevant but also have capacity to elucidate complex biological processes standard 2D monocultures unable to. Whilst liver been developed evaluate short-term genotoxicity chemicals, aim this study was develop a model could be used with regulatory vitro micronucleus (MN) following low-dose, longer-term (5 days) exposure engineered nanomaterials (ENMs). A...
The exhaust emissions from transport sector as well their air quality impacts have been steadily decreasing in urban areas due to the more stringent emission limits. secondary aerosol formation process inorganic and organic gaseous precursors emitted by traffic remains poorly characterized is likely very different for vehicles. aim of this study explore influence fuel, engine technology aftertreatment systems on potential sectors.  The measurement data utilized abstract originates...
Pollutants emitted by internal combustion engines harm human health and contribute to climate change. Diesel engines, commonly used power ships, are a significant source of these emissions. Eichler et al. (2017) identified lubricating oil as major contributor ship exhaust particles. As the shipping industry transitions decarbonized fuels, may remain organic aerosol This study highlights role minimizing in reducing emissions from ships. In this we small diesel generator produce marine fuels....
INTRODUCTIONReal Driving Emissions (RDE) methods are essential for assessing the environmental performance of Plug-In Hybrid Electric Vehicles (PHEVs), which differ significantly from Internal Combustion Engine (ICE) vehicles due to their dual powertrain systems. These systems have different operating modes, either prioritizing electric power (in this case Automatic mode) or balancing ICE use maintain battery state charge (SOCH; here mode), influence emission profiles. Such distinctions...
Vehicular particulate matter (PM) emissions have been studied widely in view of their composition and health effects, while less is known the semi-volatile compounds (SVC) fraction. Furthermore, tightening exhaust emission standards road-transport sector do not cover semi-volatiles or harmful polyaromatic hydrocarbons (PAHs) derivatives dibenzothiophenes (DBTs), nitrated (nitro-PAHs) oxygenated (oxy-PAHs) PAHs present vehicular exhaust. We PAH from PM SVC fractions collected modern cars...
Responding to the outdoor air pollution being one of gravest environmental and health hazards, mobile source emissions have been subject scrutiny reduction efforts through increased efficiency, improved fuels, engine design, combustion control, exhaust aftertreatment, traffic management. Assessment effects various improvements on human has extended from basic laboratory measurements testing under real-world (real-driving) conditions more relevant metrics than, for example, total particulate...
Black carbon (BC) emissions deteriorate air quality in cities and affect human population health. They are important also from climate point of view since atmospheric BC can absorb the solar radiation, cloud formation, decrease ground albedo when deposited to snow or ice. is emitted atmosphere large variety different anthropogenic sources. In respect exposure emissions, especially on-road traffic have had role, which has led tightening emission regulations advanced mitigation actions.In this...
Internal combustion engine emissions belong among the major anthropogenic sources of air pollution in urban areas. According to International Agency for Research on Cancer, there is sufficient evidence carcinogenicity diesel exhaust human beings. Although alternative fuels, mainly biodiesel, have recently become popular, little still known about genotoxicity from these fuels. We analysed DNA damage expressed as frequency micronuclei (MN) bronchial epithelial cells (BEAS-2B), induced by...
Abstract Activation of immune response plays an important role in the development retinal diseases. One main populations cells contributing to homeostasis are microglia, which represent a population residential macrophages. However, under pathological conditions, microglia become activated and rather support harmful inflammatory reaction angiogenesis. Therefore, targeting these could provide protection against neuroinflammation neovascularization. In recent study, we analyzed effects silver...
Cells grown in monocultures are widely used to model lung tissue. As a result of these culture conditions, cells exhibit poor morphological similarity those present vivo MucilAir™, 3-D vitro comprising human basal, goblet and ciliated cells, represents fully differentiated respiratory epithelium that can be as an alternative more realistic system. The aim our study was compare the effects short-term long-term exposure two polycyclic aromatic hydrocarbons (PAHs) — benzo[a]pyrene (B[a]P)...
The biological effects induced by complete engine emissions in a 3D model of the human airway (MucilAirTM) and bronchial epithelial cells (BEAS-2B) grown at air–liquid interface were compared. exposed for one or five days to generated Euro 5 direct injection spark ignition engine. general condition was assessed measurement transepithelial electrical resistance mucin production. cytotoxic evaluated adenylate kinase (AK) lactate dehydrogenase (LDH) activity. Phosphorylation histone H2AX used...
Road traffic emissions consist of gaseous components, particles various sizes, and chemical compounds that are bound to them. Exposure vehicle is implicated in the etiology inflammatory respiratory disorders. We investigated inflammation-related markers human bronchial epithelial cells (BEAS-2B) a 3D model airways (MucilAir™), after exposure complete extractable organic matter (EOM) from generated by ordinary gasoline (E5), gasoline-ethanol blend (E20; ethanol content 20% v/v). The...
Abstract INTRODUCTION Studies have correlated living close to major roads with Alzheimer's disease (AD) risk. However, the mechanisms responsible for this link remain unclear. METHODS We exposed olfactory mucosa (OM) cells of healthy individuals and AD patients diesel emissions (DE). Cytotoxicity exposure was assessed, mRNA, miRNA expression, DNA methylation analyses were performed. The discovered altered pathways validated using data from human population‐based Rotterdam Study. RESULTS DE...
Gasoline engine emissions have been classified as possibly carcinogenic to humans and represent a significant health risk. In this study, we used MucilAir™, three-dimensional (3D) model of the human airway, BEAS-2B, cells originating from bronchial epithelium, grown at air-liquid interface assess toxicity ordinary gasoline exhaust produced by direct injection spark ignition engine. The transepithelial electrical resistance (TEER), production mucin, lactate dehydrogenase (LDH) adenylate...
The evaluation of the frequency micronuclei (MN) is a broadly utilised approach in vitro toxicity testing. Nevertheless, specific properties nanomaterials (NMs) give rise to concerns regarding optimal methodological variants MN assay. In bronchial epithelial cells (BEAS-2B), we tested genotoxicity five types NMs (TiO2: NM101, NM103; SiO2: NM200; Ag: NM300K, NM302) using four protocols, differing time exposure and application cytochalasin-B combined with simultaneous delayed co-treatment NMs....
Polycyclic aromatic hydrocarbons (PAHs) may cause lipid peroxidation via reactive oxygen species generation. 15-F2t-isoprostane (IsoP), an oxidative stress marker, is formed from arachidonic acid (AA) by a free-radical induced oxidation. AA also be converted to prostaglandins (PG) prostaglandin-endoperoxide synthase (PTGS) NF-κB. We treated human embryonic lung fibroblasts (HEL12469) with benzo[a]pyrene (B[a]P), 3-nitrobenzanthrone (3-NBA) and extractable organic matter (EOM) ambient air...
<div class="section abstract"><div class="htmlview paragraph">Air pollution remains to be one of the leading causes premature death worldwide, with significant share attributed particulate matter and reactive nitrogen compounds from mobile sources. Due discrepancies between legislative metrics health effects, laboratory tests real driving, health-relevant metric applicable driving conditions are sought evaluate effects emerging legislation, technologies fuels. Models human lung...
Some metal nanoparticles (NP) are characterized by antimicrobial properties with the potential to be used as alternative antibiotics. However, NP may negatively impact human organism, including mesenchymal stem cells (MSC), a cell population contributing tissue growth and regeneration. To address these issues, we investigated toxic effects of selected (Ag, ZnO, CuO) in mouse MSC. MSC were treated various doses for 4 h, 24 48 h multiple endpoints analyzed. Reactive oxygen species generated...