- Polysaccharides and Plant Cell Walls
- Polysaccharides Composition and Applications
- Microbial Metabolites in Food Biotechnology
- Food composition and properties
- Enzyme Production and Characterization
- Probiotics and Fermented Foods
- Biofuel production and bioconversion
- Advanced Cellulose Research Studies
- Proteins in Food Systems
- Postharvest Quality and Shelf Life Management
- Plant Diversity and Evolution
- Glycosylation and Glycoproteins Research
- Plant and animal studies
- Marine and coastal plant biology
- Gut microbiota and health
- Plant nutrient uptake and metabolism
- Botanical Research and Applications
- biodegradable polymer synthesis and properties
- Blood Coagulation and Thrombosis Mechanisms
- Legume Nitrogen Fixing Symbiosis
- Plant Pathogenic Bacteria Studies
- Aluminum toxicity and tolerance in plants and animals
- Digestive system and related health
- Carbohydrate Chemistry and Synthesis
- Botany, Ecology, and Taxonomy Studies
Eastern Regional Research Center
2014-2024
Agricultural Research Service
2015-2024
United States Department of Agriculture
2008-2024
Pennsylvania State University
2003
Cornell University
1889-1997
The University of Texas at Austin
1987
National Institute of Diabetes and Digestive and Kidney Diseases
1984
National Institutes of Health
1984
University of Louisville
1965-1971
Texas Tech University
1966-1971
In November 2008, a group of scientists met at the 6th Meeting International Scientific Association Probiotics and Prebiotics (ISAPP) in London, Ontario, Canada, to discuss functionality prebiotics. As result this, it was concluded that prebiotic field is currently dominated by gastrointestinal events. However, future, may be case other mixed microbial ecosystems modulated approach, such as oral cavity, skin urogenital tract. Therefore, decision taken build upon current status define niche...
Fermentation properties of oligosaccharides derived from orange peel pectin were assessed in mixed fecal bacterial culture. The oligosaccharide fraction contained glucose addition to rhamnogalacturonan and xylogalacturonan pectic oligosaccharides. Twenty-four-hour, temperature- pH-controlled, stirred anaerobic batch cultures used determine the effects that products had on composition microbiota. measured through fluorescent situ hybridization changes populations, fermentation end analyzed by...
The three-dimensional structure of a complex between the pectate lyase C (PelC) R218K mutant and plant cell wall fragment has been determined by x-ray diffraction techniques to resolution 2.2 Å refined crystallographic R factor 18.6%. oligosaccharide substrate, α-D-GalpA-([1→4]-α-D-GalpA)3-(1→4)-D-GalpA, is composed five galacturonopyranose units (D-GalpA) linked α-(1→4) glycosidic bonds. PelC secreted pathogen Erwinia chrysanthemi degrades component walls in soft rot diseases. substrate...
Dissociated chromaffin cells from bovine adrenal medulla were stimulated to secrete epinephrine and dopamine beta-hydroxylase with a variety of secretagogues in study designed test the hypothesis that chemiosmotic lysis reaction isolated granules might some way be related mechanism release during exocytosis. Increasing osmotic strength incubation medium either NaCl or sucrose led suppression secretion regardless whether was induced veratridine acetylcholine. Suppression approximately...
Summary Modification of the carbohydrate structures recombinant tissue-type plasminogen activator (rt-PA) can increase or decrease its rate clearance in rabbits. When rt-PA was treated with sodium periodate to oxidize residues, decreased from 9.6 ± 1.9 ml min−1 kg−1 3.5 0.6 (mean SD, n = 5). A similar change introduced by use endo-β-N-acetyl- glucosaminidase H (Endo-H), which selectively removes high mannose asparagine-linked oligosaccharides; Endo-H-treated 5.0 0.5 kg−1. mutant produced an...
Edited by R. Reid Townsend and Arland T. Hotchkiss, Jr. Marcel Dekker, Inc., New York, 1997. xiii+648 pp. $ 65.00 (hc). ISBN: 0-8247-9822-8 The recognition that complex carbohydrates are ubiquitous throughout the plant animal kingdoms some of their recognized roles include conferring unique properties on proteins lipids has resulted in increasing interest structural functional such compounds. Therefore several monographs have appeared during last couple years to address description...
The global structure of microwave-assisted flash-extracted pectins isolated from fresh sugar beet pulp has been studied. objective was to minimize the disassembly and possibly degradation pectin molecules during extraction. These have characterized by high-performance size exclusion chromatography with light scattering, viscometric detection, atomic force microscopy (AFM). Analysis molecular parameters performed on 15 8 microm column packings. Samples analyzed packing gave weight-average...
Several studies have suggested that the emulsification properties associated with pectin obtained from sugar beet (Beta vulgaris) are due to presence of a protein-pectin complex. Nevertheless, identity protein has remained elusive. Pectin, extracted pulp by microwave-assisted extraction, and commercial sample were both subjected protease digestion trypsin. The resulting peptides separated solution ultrafiltration using 3 kDa molecular weight cutoff (MWCO) membrane analyzed matrix-assisted...
Cranberry juice has been recognized as a treatment for urinary tract infections on the basis of scientific reports proanthocyanidin anti-adhesion activity against Escherichia coli well from folklore. Xyloglucan oligosaccharides were detected in cranberry and residue remaining following commercial extraction that included pectinase maceration pulp. A novel xyloglucan was through tandem mass spectrometry analysis an ion at m/z 1055 determined to be branched, three hexose, four pentose...
A commercial high-methoxy citrus pectin was treated with a purified salt-independent methylesterase (PME) isozyme isolated from Valencia orange peel to prepare series of deesterified pectins. alkali-deesterified pectins also prepared at pH 10 under conditions permitting β-elimination. Analysis these using high-performance size exclusion chromatography (HPSEC) on-line multiangle laser light-scattering, differential viscometer, and refractive index (RI) detectors revealed no reduction in...
Abstract This study was undertaken to evaluate the effect of modified citrus pectin (MCP) on urinary excretion toxic elements in healthy individuals. MCP is a reduced molecular weight (weight‐average molar mass = 15400) that mostly linear homogalacturonan with 3.8% degree esterification and approximately 10% rhamnogalacturonan II based presence 2‐keto‐3‐deoxy‐octonic acid. Subjects ingested 15 g (PectaSol ® , EcoNugenics Inc., Santa Rosa, California 95407) each day for 5 days 20 6....