The impacts of different doses the plant growth regulator gibberellic acid (GA3) in diet on number total and differential hemocytes, frequency apoptotic, necrotic mitotic indices, encapsulation, melanization responses were investigated using greater wax moth Galleria mellonella L. (Lepidoptera: Pyralidae) larvae. Total hemocyte counts increased G. larvae at all treatment whereas GA3 application had no effect types. occurrence apoptosis, necrosis indices treated untreated last instars...

The epigenetic and immunological effects of nanoparticles (NPs), which have started to be described as nano-pollutants today, are great interest in living organisms. Particularly alumina (Al) polystyrene (PS) among the most produced NPs. Galleria mellonella larvae, an ideal model for multi-generational these NPs on global DNA methylation immune system, were used experiments. Al-NPs bought, PS-NPs by single emulsion solvent evaporation method. Al administered larvae at different...

Abstract Venom from the pupal endoparasitoid Pimpla turionellae L. (Hymenoptera: Ichneumonidae) contains a mixture of biologically active components, which display potent paralytic, cytotoxic, and cytolytic effects toward hosts. Here, we further investigate whether parasitism or envenomation by P. alters hemocyte numbers its host Galleria mellonella (Lepidoptera: Pyralidae). Total counts declined sharply in pupae larvae G. exposed to turionellae. These same cellular responses occurred when...

The biological activity of venom from Pimpla turionellae L. (Hymenoptera: Ichneumonidae) was examined in vivo toward larvae and pupae Galleriae mellonella (Lepidoptera: Pyralidae), vitro bacterial fungal cultures, as well cultured insect cells. Pupae G. were far more susceptible to the than larvae. At low doses [0.1 reservoir equivalents (VRE)], pupal abdominal mobility inhibited within 30 min, by 24 h, all injected with concentrations >0.5 VRE completely paralyzed. These same resulted an...

Parasitoids are insects that use other as hosts. They sabotage host cellular and humoral defences to promote the survival of their offspring by injecting viruses venoms along with eggs. Many pathogens parasites disrupt epigenetic mechanisms overcome immune system defences, we hypothesized parasitoids may same strategy. We used ichneumon wasp Pimpla turionellae a model idiobiont parasitoid test this hypothesis, pupae greater wax moth Galleria mellonella host. found infestation involves...

Venom from the endoparasitoid wasp Pimpla turionellae L. (Hymenoptera; Ichneumonidae) was isolated in pure form. Total protein determination indicated an average value of 0.04 μg per venom sac. The molecular weights components were estimated with reference to weight markers and proteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Analysis that primarily contains between 20 106 kDa. presence melittin apamin shown SDS-PAGE reversed-phase high-performance liquid...

In parasitoid species devoid of polydnaviruses and virus-like particles, venom appears to play a major role in suppression host immunity. Venom from the pupal endoparasitoid Pimpla turionellae L. (Hymenoptera: Ichneumonidae) has previously been shown contain mixture biologically active components, which display potent paralytic, cytotoxic, cytolytic effects toward lepidopteran dipteran hosts. The current study was undertaken investigate if parasitism and/or envenomation by P. affects...

Journal Article Effects of Gibberellic Acid on Biological Parameters the Larval Endoparasitoid Apanteles galleriae (Hymenoptera: Braconidae) Get access Fevzı Uçkan, Uçkan Search for other works by this author on: Oxford Academic Google Scholar Aylın Tüven, Tüven Er, Er Ekrem Ergın Annals Entomological Society America, Volume 101, Issue 3, 1 May 2008, Pages 593–597, https://doi.org/10.1603/0013-8746(2008)101[593:EOGAOB]2.0.CO;2 Published: 01 2008 history Received: 21 January 2007 Accepted: 17 December

Abstract: Age‐related progeny production and sex ratio of Apanteles galleriae Wilkinson, a koinobiont, solitary, larval endoparasitoid two lepidopteran species, Galleria mellonella (L.) Achoria grisella Fabr., were studied at 25± 1°C, 60 ± 5% relative humidity, photoperiod 12 : h (L D). The effect host type on fecundity the hyperparasitoid Dibrachys boarmiae (Walker), an idiobiont, gregarious pre‐pupae or pupae ectoparasitoid A. was also investigated. Total produced by showed very little...

Abstract The endoparasitic wasp Pimpla turionellae L. (Hymenoptera: Ichneumonidae) injects its pupal host with venom during oviposition. Venom from P. has previously been shown to contain a mixture of biologically active components, which display potent paralytic, cytotoxic and cytolytic effects towards lepidopteran dipteran hosts. This study was undertaken investigate if parasitism and/or envenomation by affects the encapsulation melanization responses Galleria mellonella (Lepidoptera:...

Parasitic wasps are important natural enemies of several insect pests. They use a variety methods to modulate their host for progeny develop. For example, the female wasp needs avoid or suppress immune responses by introducing venom with without virus like particles and/or polydnaviruses. The aim this paper is provide synthesis current knowledge regarding immunosuppression immunity in parasitoids that devoid symbiotic viruses. Special emphasis given through disabling hemocytes endoparasitoid...

The effect of host diet on the immature developmental time, fecundity, sex ratio, adult longevity, and size Apanteles galleriae Wilkinson, a koinobiont, solitary, early instar larval endoparasitoid lesser wax moth, Achroia grisella (F.), was investigated. All experiments related to were conducted at 25 ± 1°C, 60 5% RH, photoperiod 12:12 (L:D) h. by supplying hosts with three types natural food (blackened, dark yellowish, pure comb). change in type from blackened, comb prolonged shortened...

Journal Article Temperature and Food Source Effects on Adult Longevity of Apanteles galleriae Wilkinson (Hymenoptera: Braconidae) Get access Fevzi Uçkan, Uçkan 1 Department Biology, Faculty Science-Art, Balikesir University, Balikesir, 10100, Turkey 1E-mail: uckanf@balikesir.edu.tr Search for other works by this author on: Oxford Academic PubMed Google Scholar Ekrem Erginin Environmental Entomology, Volume 32, Issue 3, June 2003, Pages 441–446, https://doi.org/10.1603/0046-225X-32.3.441...

Abstract Venom from the endoparasitic wasp Pimpla turionellae L. (Hymenoptera: Ichneumonidae) was found to contain a complex mixture of biogenic amines, noradrenalin, phospholipase B, and several proteins peptides. The amount noradrenalin serotonin be highest in venom newly emerged wasps decreased with age. Histamine detected minute amounts comparison other components, declined increasing age parasitoids. Total peptides by reversed‐phase HPLC increased host Old‐aged (30–33 days after...

During parasitism, the ectoparasitic wasp Nasonia vitripennis (Walker) (Hymenoptera: Pteromalidae) induces a developmental arrest in host pupae that is sustained until fly either consumed by developing larvae or onset of death. Bioassays using fluids collected from female reproductive system (calyx, alkaline gland, acid and venom reservoir) indicated gland reservoir are sources arrestant inducer(s) Infrared spectroscopic analyses revealed crude acidic composed amines, peptides, proteins,...

The effects of cypermethrin on the hemocytes pupal endoparasitoid Pimpla turionellae (Hymenoptera: Ichneumonidae) feeding its host Galleria mellonella (Lepidoptera: Pyralidae) was evaluated after treatment larvae with different sub-lethal doses insecticide. Four hemocyte types; prohemocytes, plasmatocytes, granulocytes, and adipohemocytes were identified in parasitoid last instars hemolymph, based morphological criteria. Dose-dependent changes frequencies mitosis, apoptosis, micronuclei...

The effects of indol-3-acetic acid (IAA) were investigated on biological parameters Galleria mellonella (Linnaeus, 1758) (Lepidoptera: Pyralidae) and its endoparasitoid Pimpla turionellae (Linnaeus (Hymenoptera: Ichneumonidae) reared hosts treated with 50 to 10,000 ppm IAA in their diet. Percent fecundity first generation G. females increased by 12% at whereas the most effective decrease, >33%, was observed 5,000 ppm. fertility decreased >20% 1,000 All treatments displayed ±5%...

Abstract We examined the stage and sex-related changes in total lipid, fatty acids, acid composition of Apanteles galleriae Wilkinson (Hymenoptera: Braconidae) reared on Achoria grisella Fabr. (Lepidoptera: Pyralidae). The effect parasitism lipid acids host larvae was also investigated compared with parasitoids. after parasitization were degree similarity between parasitoid determined. Total A. considerably increased adults immature stages. There a significant increase levels larval pupal...

Nanoparticles (NPs) are released directly or indirectly into nature with increased production and consumption, their effects on insects, which occupy a large place in the ecosystem, of interest. There is also interest potentially toxic NPs applied to hive pests parasitoids, honey bees, host-parasitoid relationships. The influence aluminum oxide (Al2O3) biological features pest Galleria mellonella, total counts hemocyte, hemocyte types; as well endoparasitoid Pimpla turionellae were...

Abstract The nontarget effects of the plant growth regulator indol-3-acetic acid (IAA) on parasitoid development and reproduction were examined using endoparasitoid Apanteles galleriae Wilkinson (Hymenoptera: Braconidae). IAA assessed by rearing A. hosts fed different doses then measuring a range life history traits such as immature developmental time, adult longevity, body size, fecundity, sex ratios in F1 F2 generations. Wasp from egg to took 27–47 d. However, emergence parasitoids reared...