A5019145336- Virus-based gene therapy research
- Cancer Research and Treatments
- Viral Infectious Diseases and Gene Expression in Insects
- Cutaneous Melanoma Detection and Management
- CAR-T cell therapy research
- Melanoma and MAPK Pathways
- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- Wound Healing and Treatments
- Electrospun Nanofibers in Biomedical Applications
- Burn Injury Management and Outcomes
- Immune Cell Function and Interaction
- Nonmelanoma Skin Cancer Studies
- Hippo pathway signaling and YAP/TAZ
- Cutaneous lymphoproliferative disorders research
- Cancer and Skin Lesions
- Developmental Biology and Gene Regulation
- Organ and Tissue Transplantation Research
- Autoimmune and Inflammatory Disorders
- Lymphatic System and Diseases
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Protein Tyrosine Phosphatases
- Immunotoxicology and immune responses
- Dietetics, Nutrition, and Education
- Optical Coherence Tomography Applications
Helsinki University Hospital
2018-2024
University of Helsinki
2017-2024
Turku University Hospital
2022
Creative Commons
2022
Finland University
2019
Abstract Purpose: TILT-123 (igrelimogene litadenorepvec) is an oncolytic adenovirus armed with TNFa and IL2, designed to induce T-cell infiltration cytotoxicity in solid tumors. Patients Methods: TUNIMO (NCT04695327) was a single-arm, multicenter phase I dose-escalation trial assess the safety of advanced cancers refractory standard therapy. received intravenous intratumoral TILT-123. The primary endpoint by adverse events (AE), laboratory values, vital signs, electrocardiograms. Secondary...
Background: Although wound contraction is an essential part of healing, excessive can compromise healing through induction scarring and fibrosis. This in turn leads to development contractures that limit elasticity function. Major research efforts have focused on novel therapeutic approaches gain inhibitory control over contraction. Despite these efforts, the need for cost-effective, clinically feasible, effective agents inhibit remains. Methods: In this study, authors investigated effect...
Changes in the incidence of melanoma children and adolescents have been reported Europe USA recent decades.The aim this study was to examine paediatric adolescent melanomas Finland 1990-2014, associated clinical histopathological characteristics reveal temporal trends, such as changes diagnostic sensitivity Spitzoid melanomas.Information on 122 patients diagnosed with cutaneous at 0-19 years age 1990-2014 were retrieved from Finnish Cancer Registry. 73 primary archival samples re-evaluated...
Melanoma is an unpredictable, highly metastatic malignancy, and treatment of advanced melanoma remains challenging. Novel molecular markers based on the alterations in gene expression pathways activated or deactivated during progression are needed for predicting course disease already primary tumors providing new targets therapy. Here, we sought to identify genes whose melanomas correlate with patient disease‐specific survival using global profiling. Many identified potential poor prognosis...
To describe a cohort of sinonasal mucosal melanoma (SNMM) patients, and to assess if choice surgical approach (open versus endoscopic) has impact on survival.Adequate data clinical presentation, treatment, recurrence pattern were available for 58 consecutive patients treated SNMM at the Helsinki University Hospital (HUH) between 1983 2016.The 5-year disease-specific survival (DSS) was 27% overall 25% whole cohort. The 3-year DSS with curative intent endoscopic surgery comparable open (56%...
Cutaneous squamous cell carcinoma (cSCC) shows malignant behaviour in 3-4% of patients with locoregional metastases and a poor prognosis, that are difficult to predict clinically. Therefore, sentinel lymph node biopsy (SLNB) has been assessed, contradictory findings thus far. We aimed clarify the prognostic value SLNB high-risk cSCC patients.We completed retrospective clinical study amongst 63 patients, preoperatively classified as N0 primary head neck who underwent between 2001 2014 at...
Anti-PD1 treatment has improved the survival of metastatic melanoma patients, yet it is unknown which patients benefit from treatment. In this exploratory study, we aimed to understand effects anti-PD1 therapy on patients' immune system and discover characteristics that would result in successful We collected peripheral blood (PB) samples 17 immuno-oncology-naïve before after 1 3 months therapy. addition, matching tumor biopsies at time diagnosis were for tissue microarray. The complete...
Background Sentinel node biopsy (SNB) is an important step in melanoma staging and prognostication. It commonly performed for patients with intermediate thickness melanomas, based on clinicopathological features. However, only 20–25% of eventually demonstrate nodal involvement. The aim this study was to evaluate whether tissue biomarkers links biology, together parameters, could aid the prediction sentinel involvement improve selection SNB. In addition, we examined role these clinical or...
Purpose: To date, no clinical study has reported on the optimal treatment duration of PD-1 blockade in patients with metastatic melanoma. Worldwide, concern been expressed that due to high cost anti-PD-1 therapy, it is not available for all patients. After approval therapy as a first-line treatment, Helsinki University Hospital institutional board new drugs decided treat first patient cohort within limited program order offer this many possible.Patients and methods: The 40 melanoma...
We describe a case of Lichtenberg Figures (LFs) following an electrical injury from high-voltage switchgear in 47 year-old electrician. LFs, also known as ferning pattern or keraunographic markings, are pathognomonic skin sign for lightning strike injuries. Their true pathophysiology has remained mystery and only once before described electical injury. The aim was to characterise the tissue response LFs by performing untargeted non-labelled proteomics immunohistochemistry on...
Despite strong indications that interactions between melanoma and lymphatic vessels actively promote progression, the molecular mechanisms are not yet completely understood. To characterize factors of this crosstalk, we established human primary endothelial cell (LEC) cocultures with lines. Here, show coculture cells induced transcriptomic changes in LECs led to multiple their function. WNT5B, a paracrine signaling molecule upregulated upon LEC interaction, was found contribute functional...
Autologous split-thickness skin grafts (STSGs) are the standard of care for closure deep and large burns. However, perforation extensive fishnet-like expansion to achieve greater area wound coverage can lead treatment failures or esthetically poor healing outcomes scarring. The purpose this study was validate an autologous advanced therapy medicinal product (ATMP)-compliant cell suspension evaluate its efficacy promote epithelialization. Cells isolated from a piece STSG according ATMP...
Therapy options for patients with metastatic melanoma (MM) have considerably improved over the past decade. However, many still need effective therapy after unsuccessful immunotherapy, especially BRAF-negative tumors who lack option of targeted treatment second line. Therefore, elucidation efficient and personalized these is required. In this study, three patient-derived cancer cells (PDCs) were established from NRAS Q61-positive MM patients. The response PDCs five cell lines (two...
Malignant melanoma poses a clinical diagnostic problem, since large number of benign lesions are excised to find single melanoma. This study assessed the accuracy novel non-invasive technology, hyperspectral imaging, for detection. Lesions were imaged prior excision and histopathological analysis. A deep neural network algorithm was trained twice distinguish between histopathologically verified malignant melanocytic classify separate subgroups. Furthermore, 2 different approaches used:...
Abstract Because molecular memories of past inflammatory events can persist in epidermal cells, we evaluated the long-term protein expression landscapes after dermal regeneration and psoriatic inflammation. We first characterized effects two strategies on transplants indicator split-thickness skin grafts (STSGs) ten adult patients with deep burns covering more than 20% their body surface area. After fascial excision, three adjacent areas within wound were randomized to receive a permanent...
Background: Anti-PD1 therapy has proven to be effective in various cancer types, but the overall impact of treatment on immune system is not yet fully understood. Therefore, we aimed this study discover effects anti-PD1 system, especially NK and NKT cells, which are less studied, known involved antitumor events. Methods: Peripheral blood from immuno-oncology naive metastatic melanoma patients (n = 19) were obtained before first infusion anti-PD1, then 1 3 months later. From each time-point,...
Pediatric melanoma may have a different biological background and more favorable prognosis compared with in adults. The aim of this study was to investigate children adolescents the Finnish population terms incidence, clinical course, treatment, BRAFV600E-, ALK- PD-L1-positivity primary tumors.Primary tumor samples records all patients aged 0-19 years diagnosed cutaneous Finland 1990-2014 were collected using Cancer Registry database, hospitals private pathology laboratories. BRAFV600E, ALK...
2658 Background: Immune checkpoint inhibitors provide limited benefit in patients with immunologically cold tumors, characterized by a lack of T cells. This creates niche for cell-inducing agents such as TILT-123 (igrelimogene litadenorepvec, Ad5/3-E2F-D24-hTNFa-IRES-hIL2). While the 5/3 chimeric oncolytic adenovirus platform prompts potent immunogenic capacity per se, this virus has been armed TNFa and IL-2 selected based on their ability to activate attract cells into tumors. capsid...
<p><b>A</b>, Response evaluation in all injected lesions, evaluated by CT. <b>B</b>, PET. <b>C</b>, allimaged non-injected <b>D</b>, imaged Best response shown for <b>A–D</b> if patient continued to extension. <b>E</b>, Intravenous dose given versus sum SUVmax change of measured lesions on day 78. <b>F</b>, Intratumoral <b>G</b>, Overall survival the trial. <b>H</b>,...
<p>Supplementary Table S1-S5, Supplementary Figure S1-S6</p>
<p>Patient demographics.</p>
<p>Supplementary Table S1-S5, Supplementary Figure S1-S6</p>