Objective To assess the ability of urinary biomarkers IGFBP7 (insulin-like growth factor-binding protein 7) and TIMP-2 (tissue inhibitor metalloproteinase 2) to early predict acute kidney injury (AKI) in high-risk surgical patients. Introduction Postoperative AKI is associated with an increase short long-term mortality. Using for detection cellular injury, thus allowing initiation renal protection measures, may represent a new concept evaluating function. Methods In this prospective study,...

Background: We aimed to evaluate the impact of knee periprosthetic joint infection (PJI) by assessing patients’ long-term quality life and explicitly their psychological wellbeing after successful treatment. Methods: Thirty-six patients with achieved eradication PJI were included. Quality was evaluated EQ-5D SF-36 outcome instruments as well an ICD-10 based symptom rating (ISR) compared normative data. Results: At a follow-up 4.9 ± 3.5 years mean score 24.82 10.0 regarding physical health...

Treatment of meniscus tears within the avascular region represents a significant challenge, particularly in situation early osteoarthritis. Cell-based tissue engineering approaches have shown promising results. However, studies not found consensus on appropriate autologous cell source clinical situation, specifically challenging degenerative environment. The present study sought to evaluate for meniscal repair demanding setting osteoarthritis.A rabbit model was used test repair. Bone marrow...

The endogenous healing potential of avascular meniscal lesions is poor. Up to now, partial meniscectomy still the treatment choice for within area. However, large loss meniscus substance predisposes knee osteoarthritic changes. Tissue engineering techniques replacement such could be a promising alternative option. Thus, polyurethane scaffold, which already in clinical use, loaded with mesenchymal stromal cells, was analyzed repair critical defects zone. Large, approximately 7 mm broad...

Mesenchymal stem cells (MSCs) seeded on specific carrier materials are a promising source for the repair of traumatic cartilage injuries. The best supportive material has not yet been determined. As natural components cartilage's extracellular matrix, hyaluronic acid and collagen focus biomaterial research. In order to optimize chondrogenic support, we investigated three different scaffold compositions (HA)-gelatin based biomaterial.Human MSCs (hMSCs) were under vacuum composite scaffolds...

Avascular meniscus tears show poor intrinsic regenerative potential. Thus, lesions within this area predispose the patient to developing knee osteoarthritis. Current research focuses on approaches using growth factors or mesenchymal stem cells (MSCs) enhance healing capacity avascular zone. The use of MSCs especially as progenitor and a source has shown promising results. However, present studies bone-marrow-derived BMSCs in two-step procedure, which is limiting transfer clinical praxis. So,...

This study investigated the effect of thrombospondin-1 (TSP-1) on formation cartilage repair tissue in combination with stimulation by osteogenic protein-1 (OP-1). In miniature pigs, articular lesions femoral trochlea were treated microfracture technique and either received no further treatment (MFX), or additional application recombinant (MFX+OP-1), TSP-1 (MFX+TSP-1), a both proteins (MFX+TSP-1+OP-1). Six 26 weeks after surgery, degree endochondral ossification assessed histochemical...

Large osteochondral defects of the knee are a challenge for regenerative treatment. While matrix-guided autologous chondrocyte transplantation (MACT) represents successful treatment chondral defects, potential in combination with bone grafting by cancellous or block augmentation large and deep has not been evaluated.To evaluate 1- to 3-year clinical outcomes radiological results on magnetic resonance imaging (MRI) after MACT. Special emphasis is placed different methods (cancellous...

Little is known about the consequences of injuries on professional male football players' career and retirement period. The aim this study to investigate impact that players endure during their career, reasons for end post-career phase retirement.In a retrospective cross-sectional cohort study, retired German Bundesliga were investigated by standardised questionnaire analyse history sustained ending current health status suggestions future prevention strategies.Most 116 analysed (n = 73...

Anterior cruciate ligament (ACL) injuries are a common severe type of football injury at all levels play. A football-specific ACL registry providing both prospective data according to the skill level and risk factors for is lacking in literature.

Meniscus integrity is the key for joint health of knee. Therefore, main goal every meniscus treatment should be maintenance as much tissue possible. Repair tears can achieved by suture. However, in a recently published meta-analysis, long-term outcome repair showed mean failure rate 24%. In preclinical trial, locally applied mesenchymal stem cells produced differentiated meniscus-like indicating that mesenchymal-based cells, harvested from bone marrow, enhance healing critical-size tears....

Meniscus therapy is a challenging process. Besides the respective surgical procedure such as partial meniscectomy, meniscus repair, or replacement, early postoperative rehabilitation important for regeneration and return to sport work well long-term outcome. Various recommendations are available. However, current literature lacks information concerning actual in daily routine recommended by orthopedic surgeons. Thus, purpose of this study was investigate currently used standard protocols...

Focal early osteoarthritis (OA) or degenerative lesions account for 60% of treated cartilage defects each year. The current cell-based regenerative treatments have an increased failure rate treating compared to traumatic defects. Mesenchymal stem cells (MSCs) are alternative cell source OA defects, due their greater chondrogenic potential, chondrocytes. Low oxygen tension physioxia has been shown enhance MSC matrix content and could improve functional outcomes therapies. present...

Mesenchymal stem cells (MSCs) become hypertrophic in long term despite chondrogenic differentiation following the pathway of growth plate chondrocytes. This terminal leads to phenotypically unstable cartilage and was mirrored vitro by addition hypertrophy inducing medium. We investigated how intrinsic TGF-β signaling is altered pro-hypertrophic conditions.Human bone marrow derived MSC were chondrogenically differentiated 3D culture. At day 14 medium conditions changed 1. T3 withdrawal...

Therapy options for ruptured Achilles tendons need to take into account the right balance of timing, amount and intensity loading ensure a sufficient biomechanical resilience healing tendon on one hand, enable an adequate tensile stimulus other hand. However, data human after rupture during separate stages are unknown. Shear wave elastography is ultrasound technique that measures material elastic properties non-invasively, was proven have very good correlation studies. Taking advantage this...

Abstract Unicompartimental and total knee arthroplasty is one of the most successful performed operations worldwide. In last years number primary increased constantly. The aim this study to analyze rising numbers see how it used in Germany. retrospective data, provided by Federal Statistical Office Germany from 2008 2018 was analyzed, using operation codes German procedure classification system characteristics like age, sex type prosthesis. We found a slight increase unicompartimental over...

Background: Although anterior cruciate ligament reconstruction (ACLR) is a well-established procedure and standardly performed by orthopedic surgeons all over the world, there does not seem to be standard protocol for early rehabilitation. The purpose of this study was give answers following questions: (i) Does (a) use specific tendon graft, (b) potentially additional therapy concomitant pathologies influence surgeons’ choice distinct postoperative rehabilitation after ACLR? (ii) To what...

A combination of mesenchymal stem cells (MSCs) and scaffolds seems to be a promising approach for meniscus repair. To facilitate the search an appropriate scaffold material reliable objective in vitro testing system is essential. This paper introduces new scoring this purpose analyzes hyaluronic acid (HA) gelatin composite polyurethane with MSCs tissue engineering meniscus. The pore quality interconnectivity pores HA were analyzed by surface photography Berliner-Blau-BSA-solution vacuum...

The purpose of this study was to characterize the patterns a large series tibial plateau fractures with use fracture mapping, regard different types using OTA/AO and Schatzker classification. Patients intra-articular were evaluated, classification on CT scans. For axial slice that completely displayed joint plane first identified, then matched template congruently, lines identified reproduced. In addition epidemiological data (age gender), trauma mechanism (high-energy, low-energy,...

The historical treatment options for partial anterior cruciate ligament (ACL) ruptures were conservative therapy or ACL reconstruction by injured bundle entire replacement. In awareness of the regenerative potential biologic agents such as mesenchymal stem cells platelet rich plasma (PRP), healing response technique was developed to preserve with better outcomes than therapy. Further improvement this seems be obtained additional application PRP products. Thus, aim study evaluate midterm...