A5016515477- Photosynthetic Processes and Mechanisms
- Bacterial Genetics and Biotechnology
- Porphyrin Metabolism and Disorders
- Advanced Electron Microscopy Techniques and Applications
- Amino Acid Enzymes and Metabolism
- Mitochondrial Function and Pathology
- Essential Oils and Antimicrobial Activity
- Light effects on plants
- Photoreceptor and optogenetics research
- Clostridium difficile and Clostridium perfringens research
- Lipid Membrane Structure and Behavior
- Enzyme Structure and Function
- Drug Transport and Resistance Mechanisms
- bioluminescence and chemiluminescence research
- Neonatal Health and Biochemistry
- Metabolism and Genetic Disorders
- ATP Synthase and ATPases Research
- Hemoglobin structure and function
Max Planck Institute of Biophysics
2020-2024
University of Bayreuth
2018
Heteromeric amino acid transporters (HATs) comprise a group of membrane proteins that belong to the solute carrier (SLC) superfamily. They are formed by two different protein components: light chain subunit from an SLC7 family member and heavy SLC3 family. The constitutes transport whereas mediates trafficking plasma maturation functional complex. Mutation, malfunction, dysregulation HATs associated with wide range pathologies or represent direct cause inherited acquired disorders. Here we...
Abstract Iron-bound cyclic tetrapyrroles (hemes) are redox-active cofactors in bioenergetic enzymes. However, the mechanisms of heme transport and insertion into respiratory chain complexes remain unclear. Here, we used cellular, biochemical, structural computational methods to characterize structure function heterodimeric bacterial ABC transporter CydDC. We provide multi-level evidence that CydDC is a required for functional maturation cytochrome bd , pharmaceutically relevant drug target....
Sensory photoreceptors evoke numerous adaptive responses in nature and serve as light-gated actuators optogenetics to enable the spatiotemporally precise, reversible, noninvasive control of cellular events. The output optogenetic circuits can often be dialed by varying illumination quality, quantity, duration. A programmable matrix light-emitting diodes has been devised efficiently probe response systems intermittently applied light intensity pulse frequency. Circuits for light-regulated...
The treatment of infectious diseases caused by multidrug-resistant pathogens is a major clinical challenge the 21st century. membrane-embedded respiratory cytochrome bd-type oxygen reductase critical survival factor utilized pathogenic bacteria during infection, proliferation and transition from acute to chronic states. Escherichia coli encodes for two bd isoforms that are both involved in respiration under limited conditions. Mechanistic structural differences between cydABX (Ecbd-I) appCBX...
Cytochromes bd are essential for microaerobic respiration of many prokaryotes including a number human pathogens. These enzymes catalyze the reduction molecular oxygen to water using quinols as electron donors. Their importance prokaryotic survival and absence eukaryotic homologs make these enzyme ideal targets antimicrobial drugs. Here, we determined cryoEM structure menaquinol-oxidizing cytochrome bd-type reductase facultative anaerobic Actinobacterium Corynebacterium glutamicum at...
Abstract Cytochrome bd -type oxidases play a crucial role for survival of pathogenic bacteria during infection and proliferation. This the fact that there are no homologues in mitochondrial respiratory chain qualify cytochrome as potential antimicrobial target. However, few oxidase selective inhibitors have been described so far. In this report, inhibitory effects Aurachin C (AurC-type) new D (AurD-type) derivatives on oxygen reductase activity isolated terminal -I, -II bo 3 from Escherichia...
Abstract Iron-bound cyclic tetrapyrroles (hemes) are key redox-active cofactors in membrane-integrated oxygen reductases and other bioenergetic enzymes. However, the mechanisms of heme transport insertion into respiratory chain complexes remain unclear. Here, we used a combination cellular, biochemical, structural computational methods to resolve ongoing controversies around function heterodimeric bacterial ABC transporter CydDC. We provide multi-level evidence that CydDC is required for...