A5062196375- Photosynthetic Processes and Mechanisms
- Soybean genetics and cultivation
- Photoreceptor and optogenetics research
- Bacterial Genetics and Biotechnology
- ATP Synthase and ATPases Research
- Bacteriophages and microbial interactions
- Vector-borne infectious diseases
- Mitochondrial Function and Pathology
- GABA and Rice Research
- Drug Transport and Resistance Mechanisms
- Antibiotic Resistance in Bacteria
- Mosquito-borne diseases and control
- Spectroscopy and Quantum Chemical Studies
- Gut microbiota and health
- Biofuel production and bioconversion
- Electrochemical sensors and biosensors
- Microbial Metabolic Engineering and Bioproduction
- Wheat and Barley Genetics and Pathology
- Food Quality and Safety Studies
- Microplastics and Plastic Pollution
- Plant Stress Responses and Tolerance
- Plant Pathogens and Resistance
- RNA and protein synthesis mechanisms
- Water Quality Monitoring and Analysis
- biodegradable polymer synthesis and properties
China Pharmaceutical University
2025
Beijing Forestry University
2025
Max Planck Institute of Biophysics
2014-2024
Xinjiang Production and Construction Corps
2024
Shihezi University
2024
Chinese Academy of Agricultural Sciences
2024
Guangxi University
2023
Max Planck Society
2010-2014
China Tobacco
2011
Xiaomi (China)
2007
The heme-copper oxidases (HCOs) accomplish the key event of aerobic respiration; they couple O2 reduction and transmembrane proton pumping. To gain new insights into still enigmatic process, we structurally characterized a C-family HCO--essential for pathogenicity many bacteria--that differs from two other HCO families, A B, that have been analyzed. x-ray structure cbb3 oxidase Pseudomonas stutzeri at 3.2 angstrom resolution shows an electron supply system different families B. Like family-B...
Mitochondrial complex I has a key role in cellular energy metabolism, generating major portion of the proton motive force that drives aerobic ATP synthesis. The hydrophilic arm L-shaped ~1 MDa membrane protein transfers electrons from NADH to ubiquinone, providing drive pumping at distant sites arm. critical steps conversion are associated with redox chemistry ubiquinone. We report cryo-EM structure complete mitochondrial yeast Yarrowia lipolytica both deactive form and after capturing...
Mitochondrial NADH:ubiquinone oxidoreductase (complex I) is a 1-MDa membrane protein complex with central role in energy metabolism. Redox-driven proton translocation by I contributes substantially to the motive force that drives ATP synthase. Several structures of from bacteria and mitochondria have been determined, but its catalytic mechanism has remained controversial. We here present cryo-EM structure Yarrowia lipolytica at 2.1-Å resolution, which reveals positions more than 1600...
The 2 nanomotors of rotary ATP synthase, ionmotive F O and chemically active 1 , are mechanically coupled by a central rotor an eccentric bearing. Both motors rotate, with 3 steps in 10–15 . Simulation statistical mechanics has revealed that elastic power transmission is required for high rate turnover. Here, we investigate the distribution structure compliant stiff domains. compliance certain domains was restricted engineered disulfide bridges between stator, torsional stiffness (κ)...
Light energy absorption and transfer are very important processes in photosynthesis. In green sulfur bacteria light is absorbed primarily by the chlorosomes its transferred via Fenna–Matthews–Olson (FMO) proteins to a homodimeric reaction center (RC). Here, we report cryogenic electron microscopic structure of intact FMO-RC apparatus from Chlorobaculum tepidum at 2.5 Å resolution. The presents an asymmetric architecture contains two FMO trimers that show different interaction patterns with...
Cytochrome c oxidases (CcOs), members of the heme-copper containing oxidase (HCO) superfamily, are terminal enzymes aerobic respiratory chains. The cbb3-type cytochrome (cbb3-CcO) form C-family and have only central catalytic subunit in common with A- B-family HCOs. In Pseudomonas stutzeri, two cbb3 operons organized a tandem repeat. atomic structure first isoform (Cbb3-1) was determined at 3.2 Å resolution 2010 (S. Buschmann, E. Warkentin, H. Xie, J. D. Langer, U. Ermler, Michel, Science...
Pseudomonas strains have a variety of potential uses in bioremediation and biosynthesis biodegradable plastics. sp. strain phDV1, Gram-negative phenol degrading bacterium, has been found to utilize monocyclic aromatic compounds as sole carbon source via the meta-cleavage pathway. The degradation comprises an important step removal pollutants. present study aimed investigate ability phDV1 produce polyhydroxyalkanoates (PHAs) examining effect concentration on PHA production. bacterium was...
Pseudomonas sp. phDV1 is a polyhydroxyalkanoate (PHA) producer. The presence of the endogenous PHA depolymerase (phaZ) responsible for degradation intracellular one main shortages in bacterial production PHA. Further, can be affected by regulatory protein phaR, which important accumulating different PHA-associated proteins. phaZ and phaR knockout mutants were successfully constructed. We investigate from 4.25 mM phenol grape pomace wild type. was screened fluorescence microscopy, quantified...
Cytochrome aa3 from Paracoccus denitrificans and cytochrome ba3 Thermus thermophilus, two distinct members of the heme-copper oxidase superfamily, were immobilized on electrodes modified with gold nanoparticles. This procedure allowed us to achieve direct electron transfer between enzyme nanoparticles obtain evidence for different electrocatalytic properties enzymes. The pH dependence thermostability reveal that enzymes are highly adapted their native environments. These results suggest...
The broiler industry frequently encounters 2 common problems: excessive deposition of abdominal fat and poor quality meat. However, there are limited nutritional manipulation strategies to address these issues. While Anoectochilus roxburghii (Wall.) Lindl., a traditional Chinese herb, has been shown have multiple beneficial effects in humans, its potential roles chickens remain unexplored. In this study, the dietary supplementation with extract (ARE) on growth performance, deposition, meat...
Abstract Bacteria utilize small extracellular molecules to communicate in order collectively coordinate their behaviors response the population density. Autoinducer‐2 (AI‐2), a universal molecule for both intra‐ and inter‐species communication, is involved regulation of biofilm formation, virulence, motility, chemotaxis, antibiotic resistance. While many studies have been devoted understanding biosynthesis sensing AI‐2, very little information available on its export. The protein TqsA from...
The cbb3-type cytochrome c oxidases (cbb3-CcOs) are members of the heme-copper oxidase superfamily that couple reduction oxygen to translocation protons across membrane. cbb3-CcOs present only in bacteria and play a primary role microaerobic respiration, being essential for nitrogen-fixing endosymbionts some human pathogens. As frequently observed Pseudomonads, Pseudomonas stutzeri contains two independent ccoNO(Q)P operons encoding cbb3 isoforms, Cbb3-1 Cbb3-2. While crystal structure from...
Significance Multidrug and toxic compound extrusion (MATE) transporters play a significant role in the active export of drugs compounds pathogens. The transport mechanism is still not fully understood. A comprehensive phylogenetic analysis indicates that Aq_128 Aquifex aeolicus member subfamily MATE together with from ε-Proteobacteria Archaea. high-resolution crystal structure (2.0 Å) combination drug efflux assays, mutagenesis studies, docking experiments, molecular dynamics simulations...
Abstract Cytochrome bd -type oxidases play a crucial role for survival of pathogenic bacteria during infection and proliferation. This the fact that there are no homologues in mitochondrial respiratory chain qualify cytochrome as potential antimicrobial target. However, few oxidase selective inhibitors have been described so far. In this report, inhibitory effects Aurachin C (AurC-type) new D (AurD-type) derivatives on oxygen reductase activity isolated terminal -I, -II bo 3 from Escherichia...
Taraxacum kok-saghyz (TKS) is a natural rubber (NR)-producing plant and model for studying the biosynthesis of NR. Analyzing biosynthetic mechanism NR an important way to cultivate high-yield TKS varieties. JAZ proteins, which belong Jasmonate ZIM domain family, function as negative regulators in jasmonic acid (JA) signal transduction pathway. MYC2 typically regarded regulatory factor target genes proteins; proteins indirectly influence gene expression regulated by modulating its activity....