A5039409699- Neuroscience and Neuropharmacology Research
- Mitochondrial Function and Pathology
- Anesthesia and Neurotoxicity Research
- Ion channel regulation and function
- Traumatic Brain Injury and Neurovascular Disturbances
- Metabolism and Genetic Disorders
- Neuroscience and Neural Engineering
- S100 Proteins and Annexins
- bioluminescence and chemiluminescence research
- Biochemical effects in animals
- Neurological Disorders and Treatments
- Fusion and Plasma Physics Studies
- GABA and Rice Research
- Cellular Mechanics and Interactions
- Molecular Sensors and Ion Detection
- Hydrogels: synthesis, properties, applications
- Human Health and Disease
- Medicinal Plants and Neuroprotection
- Adipose Tissue and Metabolism
- Alcoholism and Thiamine Deficiency
- Neuroscience of respiration and sleep
- Nerve injury and regeneration
- Traumatic Brain Injury Research
- Medicinal Plants and Bioactive Compounds
- Graphene and Nanomaterials Applications
Scientific Center of Children's Health
2017-2024
Ministry of Health of the Russian Federation
2017-2023
Research Institute of General Pathology and Pathophysiology, the Russian Academy of Medical Sciences
2018
Russian Academy of Sciences
2013
Academy of Medical Sciences
2013
Brain injury is accompanied by neuroinflammation, accumulation of extracellular glutamate and mitochondrial dysfunction, all which cause neuronal death. The aim this study was to investigate the impact these mechanisms on Patients from neurosurgical intensive care unit suffering aneurysmal subarachnoid hemorrhage (SAH) were recruited retrospectively a respective database. In vitro experiments performed in rat cortex homogenate, primary dissociated cultures, B35 NG108-15 cell lines. We...
Glutamate excitotoxicity is implicated in the pathogenesis of numerous diseases, such as stroke, traumatic brain injury, and Alzheimer's disease, for which insulin resistance a concomitant condition, intranasal treatment believed to be promising therapy. Excitotoxicity initiated primarily by sustained stimulation ionotropic glutamate receptors leads rise intracellular Ca2+ ([Ca2+] i ), followed cascade events, delayed calcium deregulation (DCD), mitochondrial depolarization, adenosine...
Abstract Aim An impaired biological response to insulin in the brain, known as central resistance, was identified during stroke and traumatic brain injury, for which glutamate excitotoxicity is a common pathogenic factor. The exact molecular link between resistance remains unclear. To explore this issue, present study aimed investigate effects of glutamate-evoked increases intracellular free Ca 2+ concentrations [Ca ] i mitochondrial depolarisations, two key factors associated with...
Over the last decade, a number of hydrogels attracted great attention in area brain tissue engineering. The are composed hydrophilic polymers forming 3D network water. Their function is promoting structural and functional restoration damaged tissues by providing mechanical support navigating cell fate. This paper reports on neurocompatibility chitosan-g-oligo(L,L-lactide) copolymer hydrogel with primary rat cortical neuron culture. was produced molding technique base photocurable composition...
Glutamate excitotoxicity is involved in the pathogenesis of many disorders, including stroke, traumatic brain injury, and Alzheimer's disease, for which central insulin resistance a comorbid condition. Neurotoxicity glutamate (Glu) primarily associated with hyperactivation ionotropic N-methyl-D-aspartate receptors (NMDARs), causing sustained increase intracellular free calcium concentration ([Ca2+]i) synchronous mitochondrial depolarization an superoxide anion radical (O2-•) production....
In vitro models of traumatic brain injury (TBI) help to elucidate the pathological mechanisms responsible for cell dysfunction and death. To simulate in mechanical trauma, primary neuroglial cultures were scratched during different periods network formation. Fluorescence microscopy was used measure changes intracellular free Ca2+ concentration ([Ca2+]i) mitochondrial potential (ΔΨm) a few minutes later on days 3 7 after scratching. An increase [Ca2+]i decrease ΔΨm observed ~10 s cells...
Currently, stem cells technology is an effective tool in regenerative medicine. Cell therapy based on the use of stem/progenitor to repair or replace damaged tissues organs. This approach can be used treat various diseases, such as cardiovascular, neurological and injuries origins. The mechanisms cell therapeutic action are integration graft into tissue (replacement effect) ability secrete biologically active molecules cytokines, growth factors other signaling that promote regeneration...
The scratch test is used as an experimental in vitro model of mechanical damage to primary neuronal cultures study the mechanisms cell death damaged areas. involvement NMDA receptors processes leading delayed death, due calcium dysregulation and synchronous mitochondrial depolarization, has been previously demonstrated. In this study, we explored neuroregenerative potential Pro-Gly-Pro (PGP)—an endogenous regulatory peptide with neuroprotective anti-inflammatory properties a mild...
Glutamate excitotoxicity is implicated in the pathogenesis of many disorders, including stroke, traumatic brain injury, and Alzheimer’s disease, for which central insulin resistance a comorbid condition. Massive glutamate release primarily through ionotropic N-methyl-D-aspartate receptors (NMDARs) causes sustained rise [Ca2+]i, followed by mitochondrial depolarization an increase intracellular O2• (superoxide) production. Recently, we found that protected neurons against...
Актуальность. При исследованиях внутриклеточного сигналинга и межнейрональной передачи сигнала в мозге норме при патологии все большее применение находят трансгенные животные, экспрессирующие нейронах флуоресцентный Са2+-сенсор. Вместе с тем, пока недостаточно исследовано, насколько изменения кальциевого гомеостаза интактном соотносятся гораздо более подробно изученными изменениями этого важнейшего параметра нейроглиальных культурах, служащих модельными системами живого мозга. Целью работы...