A5080011655- Liver physiology and pathology
- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Trauma Management and Diagnosis
- Organ Transplantation Techniques and Outcomes
- Pleural and Pulmonary Diseases
- Immune cells in cancer
- Liver Disease Diagnosis and Treatment
- Congenital Diaphragmatic Hernia Studies
- Electrospun Nanofibers in Biomedical Applications
- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- Magnetic properties of thin films
- Neonatal Respiratory Health Research
- Neurogenesis and neuroplasticity mechanisms
- Phagocytosis and Immune Regulation
- Anesthesia and Neurotoxicity Research
- Macrophage Migration Inhibitory Factor
- Airway Management and Intubation Techniques
- Food Industry and Aquatic Biology
- Angiogenesis and VEGF in Cancer
- Pancreatic function and diabetes
- Theoretical and Computational Physics
- Restraint-Related Deaths
- Abdominal Trauma and Injuries
Peoples' Friendship University of Russia
2018-2024
Krasnoyarsk State Agrarian University
2024
Pirogov Russian National Research Medical University
2009-2024
Ministry of Health of the Russian Federation
2015-2024
Moscow Research Institute" Nemchinovka "
2024
Institute of Molecular and Cell Biology
2024
Research Institute of Human Morphology
2014-2022
National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I.Kulakov of the Ministry of Healthcare of the Russian Federation
2015-2021
Lomonosov Moscow State University
2003-2021
Perm National Research Polytechnic University
2015-2020
Abstract Background Mesenchymal stromal/stem cells derived from human umbilical cord (UC-MSCs) uniquely combine properties of embryonic and postnatal MSCs may be the most acceptable, safe, effective source for allogeneic cell therapy e.g. therapeutic angiogenesis. In this report we describe pro-angiogenic UC-MSCs as manifested in vitro . Methods were isolated Wharton’s jelly by enzymatic digestion. Presence soluble forms VEGF-A UC-MSC-conditioned media was measured ELISA. Effects conditioned...
Transplantation of various types stem cells as a possible therapy for stroke has been tested years, and the results are promising. Recent investigations have shown that administration conditioned media obtained after cell cultivation can also be effective in central nervous system pathology (hypothesis their paracrine action). The aim this study was to evaluate therapeutic effects medium hiPSC-derived glial neuronal progenitor rat middle cerebral artery occlusion model ischemic stroke....
There is still much to learn about the epigenetic mechanisms controlling gene expression during carcinogenesis. When researching aberrant DNA methylation, active proliferative tumor cells from head and neck squamous cell cancer (HNSCC) can be used as a model. The aim of study was investigate methylation status
Macrophages are a central component of innate immunity that play an important role in the defense organism. highly plastic and activated by interaction with other cells environmental factors. In this work, we study effect lipopolysaccharide on macrophages derived from two most polar (CD14+ CD16+ monocytes) as well intermediate subset blood monocytes healthy donors assess what happens to prone polarization transcriptomic proteomic level. It has been shown that, according primary...
In many clinical cases of extensive liver resection (e.g. due to malignancy), the residual portion is too small maintain body homeostasis. The resulting acute failure associated with compensatory growth inhibition, which a typical manifestation 'small for size' syndrome. study investigates possible causes delayed onset hepatocyte proliferation after subtotal hepatectomy (80% resection) in rats. data indicate that inhibition correlates upregulation Tnf gene expression and low content...
Macrophages are important regulators of liver repair. Participation migratory monocytes/macrophages in regeneration hepatic tissues after resection remains disputable. In mouse the promotes migration Ly6C+CD11b+ to remnant accompanied by a reduction its CD206 + macrophage content. Macrophage proliferation within reaches maximum on day 3 surgery. Corresponding macro- and microtranscriptomic profiles macrophages cannot be unambiguously defined as pro- or anti-inflammatory. Their typical...
Background: Macrophages play a key role in liver regeneration. The fates of resident macrophages after 70% resection are poorly investigated. In this work, using the MARCO macrophage marker (abbreviated from receptor with collagenous structure), we studied dynamics mouse resection. Methods: BALB/c male mice, model regeneration was reproduced. markers CD68, TIM4, and were immunohistochemically by Western blot. Results: number MARCO- CD68-positive regenerating increased 1 day 3 days resection,...
In the central nervous system and in liver, macrophage populations are represented exclusively by descendants of hematopoietic progenitor cells yolk sac. The reasons for such differential distribution macrophages not fully understood. We found that, as can be judged corresponding changes expression CD86 CD163 markers, transient monocytic lineage more sensitive to activating stimuli. two have distinct patterns gene expression, which is particularly noticeable M1- M2-associated genes. For...
Macrophage populations in most mammalian organs consist of cells different origin. Resident macrophages originate from erythromyeloid precursors the yolk sac wall; maintenance numbers such postnatal ontogenesis is practically independent bone marrow haematopoiesis. The largest resident embryonic origin are found central nervous system (microglia) and liver (Kupffer cells). In contrast, skin dermis mucous membranes become predominantly colonized by marrow-derived monocytes that show...
Abstract The objective of this study was to evaluate physical, mechanical, and biological properties the polydioxanone (PDO) monofilament meshes polyglycolide (PGA) polyfilament in comparison with Permacol ® implants. In rat experimental model, a 1.5 × 2.0 cm defect abdominal wall reconstructed by using surgical implant or knitted produced from either PDO monofilament, PGA multifilament. sites were assessed for tensile strength extents material resorption, host inflammatory response tissue...
AIMTo investigate the influence of umbilical cordderived multipotent stromal cells (MSCs) on recovery liver after subtotal resection, that is, removal 80% organ mass, a renowned model small-for-size remnant syndrome.
Proliferation of hepatocytes is known to be the main process in hepatectomy-induced liver regrowth; however, cases extensive loss it may insufficient for complete recovery unless supported by some additional sources e.g. mobilization undifferentiated progenitors. The study was conducted on rat model 80% subtotal hepatectomy; objective evaluate contributions and resident progenitor cells hepatic tissue via monitoring specific mRNA and/or protein expression levels a panel genes implicated...
Cell therapy is increasingly recognized as a beneficial practice in various cardiac conditions, but its fundamentals remain largely unclear. The fates of transplanted multipotent stromal cells postinfarction microenvironments are particularly understudied. To address this issue, labeled were infused into rat myocardium at day 30 after myocardial infarction, against the background cardiosclerosis. Therapeutic effects transplantation assessed by an exercise tolerance test. Histological...
Functional and anatomical connection between the liver spleen is most clearly manifested in various pathological conditions of (cirrhosis, hepatitis). The mechanisms interaction two organs are still poorly understood, as there have been practically no studies on influence exerted by normal liver. Mature male Sprague-Dawley rats 250–260 g body weight, 3 months old, were splenectomized. highest numbers Ki67+ hepatocytes splenectomized observed at 24 h after surgery, simultaneously with index...
Glioblastoma is the most common brain cancer. Ephrins and their Eph receptors play important roles in development of central nervous system regulation cancer cell migration invasion. In a search for receptor complexes, we used tandem affinity purification based interaction screening with tagged ephrins A1, A3 A4 combined protein identification by mass-spectrometry U251MG glioblastoma cells. bound to receptors, mainly EphA2 these Integrin α3 was identified complexes ephrin-As. Soluble...
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